Laccase-mimicking activity of octahedral MnO nanoparticles and fluorescence of carbon dots as dual-mode signals for the specific detection of arsenic(V) in environmental water samples.

The detrimental growth of water pollutants such as heavy metals has become a life-threatening problem in the modern era. Challenges remain in the development of rapid and accurate methods for detecting pentavalent arsenic [As(V)] in environmental water. The octahedral MnO nanoparticles (NPs) did not display excellent laccase-mimicking catalytic activity, whereas the adsorbed As(V) on the surface significantly enhanced the catalytic activity.

A stable colorimetric biosensor for highly selective detection of malathion residue in food based on aptamer-regulated laccase-mimic activity.

Malathion causes a serious threat to human health due to its widespread use in the environment. Herein, a novel and stable smartphone-integrated colorimetric biosensor for malathion detection is firstly established based on aptamer-enhanced laccase-mimicking activity. The results indicate that the M17-F aptamer can increase the affinity of AgO nanoparticles to the substrate 2,4-dichlorophenol and enhance their laccase-mimicking activity.

Facile colorimetric sensor using oxidase-like activity of octahedral AgO particles for highly selective detection of Pb(II) in water.

Pb(II) is a prevalent heavy metal ion classified as a 2B carcinogen. Excessive intake of Pb(II) in the human body can damage the central nervous system, kidneys, liver, and immune system, leading to permanent brain damage, anemia, and cancer. Colorimetry can be applied to rapidly determine Pb(II) residues, but there are still many challenges in the accuracy and sensitivity of detection.

Rapid colorimetric sensor for ultrasensitive and highly selective detection of Fumonisin B1 in cereal based on laccase-mimicking activity of silver phosphate nanoparticles.

AgPO nanoparticles (NPs) was prepared through a facile coprecipitation method, and was first found to have excellent laccase-mimicking catalytic activity. The study confirms that Fumonisin B1 (FB1) can effectively hinder the production of superoxide anion (O) between AgPO NPs and dissolved oxygen, and further inhibit laccase-mimicking activity of AgPO NPs. Thus, a novel rapid colorimetric sensor for FB1 analysis in cereal was first established using laccase-mimicking activity as sensing signal.

Porous-nanozyme-based colorimetric sensor for rapid detection of kanamycin in foods under neutral condition.

Most colorimetric methods based on nanozymes need to have excellent performance under acidic condition, so they are currently facing some challenges in the field of food hazard detection. Herein, a facile and rapid colorimetric sensor for kanamycin (KAN) detection in foods under neutral condition has been designed using the peroxidase-mimic activity of porous nanozyme like Co O nanodisk. Further investigations showed that the interaction mechanism between porous Co O nanodisk and substrates belongs to a ping-pong model, and the inhibition type of KAN on the peroxidase-mimic activity is noncompetitive inhibition.

Gut bacteria reflect the adaptation of (Orthoptera: Rhaphidophoridae) to the cave.

The gut microbiota is essential for the nutrition, growth, and adaptation of the host. , a scavenger that provides energy to the cave ecosystem, is a keystone species in the karst cave in China. It inhabits every region of the cave, regardless of the amount of light.

Aptamer-enhanced the Ag(I) ion-3,3',5,5'-tetramethylbenzdine catalytic system as a novel colorimetric biosensor for ultrasensitive and selective detection of paraquat.

A facile and simple colorimetric biosensor was first established for paraquat (PQ) detection based on the aptamer-enhanced oxidation process of 3,3',5,5'-tetramethylbenzidine (TMB) by Ag. The study confirmed that the interaction of PQ-15 aptamer with Ag accelerates the electron transfer from the aptamer-Ag complex to dissolved oxygen, which enhances the release of superoxide anion radicals (Ȯ) and facilitates the catalytic oxidation of the chromogenic substrate. PQ-15 aptamer will preferentially bind to PQ molecules, resulting in no further enhancement of the catalytic activity of Ag.

The complete mitochondrial genome of (Hemiptera: Pentatomidae).

The mitochondrial genome of has been decoded using Illumina sequencing. The mitogenomic size is 15,368 bp with 42.97% A, 33.

The complete mitochondrial genome of (Diptera: Syrphidae).

The mitochondrial genome of has been decoded by Illumina sequencing. The mitogenomic size is 16,229 bp with 40.96% A, 40.

MicroRNA-361-3p promotes human breast cancer cell viability by inhibiting the E2F1/P73 signalling pathway.

Analysis of the microRNA (miRNA) expression signature of breast cancer based on RNA sequencing demonstrated that miR-361-3p was significantly upregulated in breast cancer tissues. miR-361-3p is a novel miRNA, and its role in breast cancer is currently unclear. The aim of the present study was to investigate the functions of miR-361-3p in breast carcinoma.

Epidural Analgesia Improves Postoperative Pain Control but Impedes Early Discharge in Patients Undergoing Pancreatic Surgery.

Objectives: The aim of this study was to evaluate the impact of epidural analgesia (EA) on postoperative length of stay (LOS), expeditious discharge, and pain relief after pancreaticoduodenectomy (PD) and distal pancreatectomy (DP).

Methods: Retrospective reviews of 2014-2015 American College of Surgeons National Surgical Quality Improvement Program databases and our institutional pancreatic surgery database were conducted.

Results: On univariate analysis, EA was associated with statistically significant longer lengths of stay for both PD and DP.

Micro-Incision of the Cystic Duct Confluence in Laparoscopic Common Bile Duct Exploration for Elderly Patients with Choledocholithiasis.

Common bile duct (CBD) stones are common in elderly patients. The laparoscopic transcystic approach with micro-incision of the cystic duct confluence in common bile duct exploration (LTM-CBDE) is a modified laparoscopic transcystic approach. Its safety and efficacy have not been studied in elderly patients with secondary choledocholithiasis.

Protection Against Infection in BALB/c Mouse Model by Oral Administration of Multivalent Epitope-Based Vaccine of Cholera Toxin B Subunit-HUUC.

Vaccination is an increasingly important alternative approach to control infection, since resistance to previously efficacious antibiotic regimens is increased, and eradication treatment for upper gastrointestinal diseases is becoming less successful. Fortunately, an efficient oral monovalent vaccine has been developed. However, compared with monovalent vaccines, multivalent vaccines have the potential to induce more effective and comprehensive protection against infection.

Hemostatic Effect of Hemocoagulase Agkistrodon on Surgical Wound in Breast Cancer Surgery.

Objective To evaluate the hemostatic effect of hemocoagulase agkistrodon on surgical wound in breast cancer surgery. Methods Totally 60 patients undergoing breast cancer surgery were enrolled in this prospective,randomized,double-blinded,and controlled study. All the patients met the inclusion and exclusion criteria and signed the informed consent.

AFP mRNA level in enriched circulating tumor cells from hepatocellular carcinoma patient blood samples is a pivotal predictive marker for metastasis.

Circulating tumor cells (CTCs) quantification may be helpful for evaluating cancer dissemination, predicting prognosis and assessing therapeutic effectiveness and safety. In the present study, CTCs from blood samples of 72 patients with hepatocellular carcinoma (HCC) were enriched with anti-EpCAM nanoparticles. AFP mRNA level was detected by nested polymerase chain reaction (PCR) after enrichment of CTCs from HCC blood samples at 0, 3, 6, 9 and 12 months after hepatectomy, respectively.

[Association of bacterial diversity in city area of Nanming river with environmental factors].

Objective: The aim of this study was to analyze the bacterial diversity of urban section of Nanming river as well as to study relationship between the bacterial diversity and environmental factors.

Methods: The high throughput sequencing on PCR-amplified 16S rDNA V4 fragments was used to determine the bacterial diversity of samples from five sites of Nanming river. The ordination technique of redundancy analysis was used to analyze the effects of the environmental factors on bacterial community composition.

[The New Bacteria Expressing Recombinant Multi-epitope Vaccine against Helicobacter pylori and Its Microbiological Characteristics].

Ojective: To construct the engineering bacteria with recombinant plasmid expressing the multi-epitope vaccine which composed of Helicobacter pylori urea membrane channel protein (UreI), Helicobacter pylori urease B subunit (UreB) and cholera toxin B subunit (CTB), and then to study it's microbiological characteristics.

Methods: The sequence contains some dominant epitopes of Helicobacter pylori UreI and UreB was designed, and ctB was added at the N-terminal, all the sequence were linked by flexible linkers. Codon optimization was done according to Escherichia coli (E.

Upconversion fluorescence-SERS dual-mode tags for cellular and in vivo imaging.

Fluorescent-surface enhanced Raman scattering (F-SERS) dual mode tags showed great potential for bioimaging due to the combined advantages of intuitive, fast imaging of fluorescence and multiplex capability of SERS technique. In previously reported F-SERS tags, organic fluorescent dyes or quantum dots were generally selected to generate fluorescence signal. Herein, we reported the first proof-of-concept upconversion fluorescence (UCF)-SERS dual mode tags based on near infrared (NIR) laser (980 nm) excited upconversion nanoparticles (UCNPs) for live-cell and in vivo imaging.