Intelligent identification of foodborne pathogenic bacteria by self-transfer deep learning and ensemble prediction based on single-cell Raman spectrum.

Foodborne pathogenic infections pose a significant threat to human health. Accurate detection of foodborne diseases is essential in preventing disease transmission. This study proposed an AI model for precisely identifying foodborne pathogenic bacteria based on single-cell Raman spectrum.

Buoyant Metal-Organic Framework Corona-Driven Fast Isolation and Ultrasensitive Profiling of Circulating Extracellular Vesicles.

Accurately assaying tumor-derived circulating extracellular vesicles (EVs) is fundamental in noninvasive cancer diagnosis and therapeutic monitoring but limited by challenges in efficient EV isolation and profiling. Here, we report a bioinspired buoyancy-driven metal-organic framework (MOF) corona that leverages on-bubble coordination and dual-encoded surface-enhanced Raman scattering (SERS) nanotags to streamline rapid isolation and ultrasensitive profiling of plasma EVs in a single assay for cancer diagnostics. This integrated bubble-MOF-SERS EV assay (IBMsv) allows barnacle-like high-density adhesion of MOFs on a self-floating bubble surface to enable fast isolation (2 min, near 90% capture efficiency) of tumor EVs via enhanced EV-MOF binding.

Exosome-tuned MOF signal amplifier boosting tumor exosome phenotyping with high-affinity nanostars.

The natural phospholipid structure imparts exosomes with not only cargo protection, but rich sites for coordination with metal-organic frameworks (MOFs) to assemble functional nanocomplexes, such as signal amplifiers. Here, we exploit exosomes to tune MOF signal amplifiers (Exo-MOF) for ultrasensitive phenotyping of tumor-derived exosomes (tExo) based on self-driven coordination assembly and high-affinity nanostars. Exo-MOF leverages the specific coordination interaction between exosome and MOF that cages abundant redox molecules to assemble a super-redox signal amplifier.

CRISPR/dCas9-Mediated Specific Molecular Assembly Facilitates Genotyping of Mutant Circulating Tumor DNA.

Breakthroughs in circulating tumor DNA (ctDNA) analysis are critical in tumor liquid biopsies but remain a technical challenge due to the double-stranded structure, extremely low abundance, and short half-life of ctDNA. Here, we report an electrochemical CRISPR/dCas9 sensor (E-dCas9) for sensitive and specific detection of ctDNA at a single-nucleotide resolution. The E-dCas9 design harnesses the specific capture and unzipping of target ctDNA by dCas9 to introduce a complementary reporter probe for specific molecular assembly and signal amplification.

Exposure of Salmonella enterica serovar 1,4,[5],12:i:- to benzalkonium chloride leads to acquired resistance to this disinfectant and antibiotics.

Aims: Disinfectants such as benzalkonium chloride (BC), extensively used in animal farms and food-processing industries, contribute to the development of adaptive and cross-resistance in foodborne pathogens, posing a serious threat to food safety and human health. The purpose of this study is to explore whether continuous exposure of Salmonella enterica serovar 1,4,[5],12:i:- (S. 1,4,[5],12:i:-) to sublethal concentrations of BC could result in acquired resistance to this agent and other environmental stresses (e.

Effects of antibiotic-induced resistance on the growth, survival ability and virulence of Salmonella enterica.

Salmonella enterica is an important foodborne pathogen that constitutes a major health hazard. The emergence and aggravation of antibiotic-resistant Salmonella has drawn attention widely around the world. Conducting a risk assessment of antibiotic-resistant foodborne pathogens throughout the food chain is a pressing requirement for ensuring food safety.

Emergence of a Hybrid IncI1-Iα Plasmid-Encoded Conferring Resistance to Cephalosporins in Serovar Enteritidis.

The increasing resistance to cephalosporins in poses a serious threat to public health. In our previous study, the gene, a new variant, was first reported in serovar Enteritidis ( Enteritidis). Here, we further analyzed the genome characterization, transferability, and resistance mechanism of one Enteritidis isolate (SJTUF14523) carrying from an outpatient in 2016 in Xinjiang, China.

Bioorthogonal Microbubbles with Antifouling Nanofilm for Instant and Suspended Enrichment of Circulating Tumor Cells.

Integrating clinical rare cell enrichment, culture, and single-cell phenotypic profiling is currently hampered by the lack of competent technologies, which typically suffer from weak cell-interface collision affinity, strong nonspecific adsorption, and the potential uptake. Here, we report cells-on-a-bubble, a bioinspired, self-powered bioorthogonal microbubble (click bubble) that leverages a clickable antifouling nanointerface and a DNA-assembled sucker-like polyvalent cell surface, to enable instant and suspended isolation of circulating tumor cells (CTCs) within minutes. Using this biomimetic engineering strategy, click bubbles achieve a capture efficiency of up to 98%, improved by 20% at 15 times faster over their monovalent counterparts.

Distribution-based maximum likelihood estimation methods are preferred for estimating Salmonella concentration in chicken when contamination data are highly left-censored.

Salmonella is a common chicken-borne pathogen that causes human infections. Data below the detection limit, referred to as left-censored data, are frequently encountered in the detection of pathogens. The approach of handling the censored data was regarded to affect the estimation accuracy of microbial concentration.

Primer exchange reaction-amplified protein-nucleic acid interactions for ultrasensitive and specific microRNA detection.

Protein-nucleic acid interactions are not only fundamental to genetic regulation and cellular metabolism, but molecular basis to artificial biosensors. However, such interactions are generally weak and dynamic, making their specific and sensitive quantitative detection challenging. By using primer exchange reaction (PER)-amplified protein-nucleic acid interactions, we here design a universal and ultrasensitive electrochemical sensor to quantify microRNAs (miRNAs) in blood.

Bottom-Up Signal Boosting with Fractal Nanostructuring and Primer Exchange Reaction for Ultrasensitive Detection of Cancerous Exosomes.

Exosomes are emerging as promising biomarkers for cancer diagnosis, yet sensitive and accurate quantification of tumor-derived exosomes remains a challenge. Here, we report an ultrasensitive and specific exosome sensor (NPExo) that initially leverages hierarchical nanostructuring array and primer exchange reaction (PER) for quantitation of cancerous exosomes. This NPExo uses a high-curvature nanostructuring array (bottom) fabricated by single-step electrodeposition to enhance capturing of the target exosomes.

A review of potential antibacterial activities of nisin against Listeria monocytogenes: the combined use of nisin shows more advantages than single use.

Listeria monocytogenes is a foodborne pathogen causing serious public health problems. Nisin is a natural antimicrobial agent produced by Lactococcus lactis and widely used in the food industry. However, the anti-L.

A Meta-Analysis and Systematic Review of Response to Sanitizer Treatments.

Listeria monocytogenes is a ubiquitous organism that can be found in food-related environments, and sanitizers commonly prevent and control it. The aim of this study is to perform a meta-analysis of L. monocytogenes response to sanitizer treatments.

Detecting the Mechanism of Action of Antimicrobial Peptides by Using Microscopic Detection Techniques.

Increasing antibiotic resistance has shifted researchers' focus to antimicrobial peptides (AMPs) as alternatives to antibiotics. AMPs are small, positively charged, amphipathic peptides with secondary helical structures. They have the ability to disrupt the bacterial membrane and create wedges due to electrostatic differences.

Phylogenomic Analysis of Salmonella enterica Serovar Indiana ST17, an Emerging Multidrug-Resistant Clone in China.

Salmonella enterica serovar Indiana ( Indiana) is an extremely expanded foodborne pathogen in China in recent years. This study aimed to elucidate the national prevalence and phylogenomic characterization of this pathogen in China. Among 5, 287 serotyped Salmonella isolates collected during 2002 to 2018, 466 Indiana isolates were found in 15 provinces, and 407 were identified to be ST17, and the rest were ST2040.

[Placenta-specific virulence factors involved in infection].

(LM) is a food-borne pathogen that can cause listeriosis. Pregnant women are main target population of listeriosis due to pregnancy-associated immune deficiency and unique intracellular infection ability of LM to non-phagocytic cells. LM can cross the placental barrier and cause significant harm to the fetus, including premature birth, miscarriage and even stillbirth.

Machine learning approach for predicting single cell lag time of Salmonella Enteritidis after heat and chlorine treatment.

The importance of single-cell variability is increasingly prominent with the developments in foodborne pathogens modeling. Traditional predictive microbiology model cannot accurately describe the growth behavior of small numbers of cells due to individual cell heterogeneity. The objective of the present study was to develop predictive models for single cell lag times of Salmonella Enteritidis after heat and chlorine treatment.

Antibiotic Resistance of Typhimurium Monophasic Variant 1,4,[5],12:i:-in China: A Systematic Review and Meta-Analysis.

Antibiotic resistance in is a global public health problem. serovar 1,4,[5],12:i:- (. 1,4,[5],12:i:-), a monophasic variant of Typhmurium, is one of the leading serovars in several countries.

A sustainable and efficient recycling strategy of feather waste into keratin peptides with antimicrobial activity.

The study aimed to propose an efficient and eco-friendly strategy to improve the utilization of feather waste and converting it into high-valued antimicrobial products. Under the synergistic effect of instant catapult steam explosion (ICSE) (1.5 MPa-120 s), over 90% of chicken feather powder (CFP) was degraded into soluble peptides via keratinolysis within 3 h, about 90% of which were smaller than 3 kDa, indicating an overwhelming advantage than general proteolysis.

Leaf Intracellular Water Transport Rate Based on Physiological Impedance: A Possible Role of Leaf Internal Retained Water in Photosynthesis and Growth of Tomatoes.

Water consumed by photosynthesis and growth rather than transpiration accounts for only 1-3% of the water absorbed by roots. Leaf intracellular water transport rate (LIWTR) based on physiological impedance (Z) provides information on the transport traits of the leaf internal retained water, which helps determine the intracellular water status. plants were subjected to five different levels of relative soil water content (SWC ) (e.