Association of IL-16 gene polymorphisms with sporadic Parkinson's disease in a Han Chinese population.

This study was performed to investigate the genetic association of single-nucleotide polymorphisms (SNPs) in the interleukin-16 (IL-16) gene with the risk of Parkinson's disease (PD) in a Chinese Han population. Genotyping for the rs11556218 T/G, rs1131445 T/C and rs4072111 C/T polymorphisms of IL-16 was performed using the PCR-RFLP method in 405 patients with PD and 405 healthy matched individuals. Statistically significant difference for rs4072111 could be observed in both additive model (TC vs.

Serious bacterial infections and antibiotic prescribing in primary care: cohort study using electronic health records in the UK.

Objective: This study evaluated whether serious bacterial infections are more frequent at family practices with lower antibiotic prescribing rates.

Design: Cohort study.

Setting: 706 UK family practices in the Clinical Practice Research Datalink from 2002 to 2017.

Genome-wide analysis reveals the association between alternative splicing and DNA methylation across human solid tumors.

Background: Dysregulation of alternative splicing (AS) is a critical signature of cancer. However, the regulatory mechanisms of cancer-specific AS events, especially the impact of DNA methylation, are poorly understood.

Methods: By using The Cancer Genome Atlas (TCGA) SpliceSeq and TCGA data for ten solid tumor types, association analysis was performed to characterize the potential link between cancer-specific AS and DNA methylation.

Genome-wide methylation and expression profiling identify methylation-associated genes in colorectal cancer.

To identify methylation-associated genes in the carcinogenesis of colorectal cancer (CRC). Genome-wide patterns of DNA methylation and gene expression in CRC tissues and adjacent normal tissues were determined and further validated in The Cancer Genome Atlas data and Chinese CRC patients, respectively. Gene overexpression and knockdown cells were constructed to investigate their biological roles in CRC.

Mechanism research of miR-34a regulates Axl in non-small-cell lung cancer with gefitinib-acquired resistance.

Background: To investigate the regulatory mechanism behind miR-34a-altered Axl levels in non-small-cell lung cancer (NSCLC) with gefitinib-acquired resistance.

Methods: The expression of miR-34a, Axl, Gas6 and related downstream signaling proteins in the EGFR mutant NSCLC cell lines were determined by qRT-PCR and Western blot; PC9-Gef-miR-34a and HCC827-Gef-miR-34a cells were established by transfecting the parent cells with a miR-34a overexpressing virus, then the expression of Axl, Gas6 and the downstream channel-related proteins were also compared in PC9-Gef-miR-34a and HCC827-Gef-miR-34a and drug-resistant strains. The survival rate of the cells were measured by CCK8 assay.

Inhibition of Long Noncoding RNA CRNDE Increases Chemosensitivity of Medulloblastoma Cells by Targeting miR-29c-3p.

Long noncoding RNA CRNDE (CRNDE) recently emerged as a carcinogenic promoter in various cancers including medulloblastoma. However, the functions and molecular mechanisms of CRNDE to the acquired drug resistance of medulloblastoma are still unclear. The transcript levels of CRNDE were examined in four medulloblastoma cell lines exposed to cisplatin treatment, and IC values were calculated.

Sinomenine's protective role and mechanism in stress load-induced heart failure.

Objectives: This study is designed to investigate the effects and mechanisms of sinomenine (Sin) in stress load-induced heart failure in mice.

Methods: We used aortic constriction (AB) to cause pressure overload as our heart failure model. Sin was received in mice as the treatment group.

Effects of phytic acid and lysozyme on microbial composition and quality of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.

This study investigated the effect of phytic acid and lysozyme on the microbial composition and quality of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C. The control, 0.5 mg/mL lysozyme-treated fillets (T1), 0.

p38-regulated FOXC1 stability is required for colorectal cancer metastasis.

Aberrant expression of forkhead box C1 (FOXC1) promotes tumor metastasis in multiple human malignant tumors. However, the upstream modulating mode and downstream molecular mechanism of FOXC1 in metastasis of colorectal cancer (CRC) remain unclear. Herein we describe a systematic analysis of FOXC1 expression and prognosis in CRC performed on our clinical data and public databases, which indicated that FOXC1 upregulation in CRC samples was significantly associated with poor prognosis.

Pneumonia incidence trends in UK primary care from 2002 to 2017: population-based cohort study.

Increasing hospital admissions for pneumonia have been reported recently but it is not known whether pneumonia incidence rates have increased in the community. To determine whether incidence rates of pneumonia increased in primary care in the United Kingdom from 2002 to 2017, an open cohort study was conducted using electronic health records from the UK Clinical Practice Research Datalink. Clinically diagnosed pneumonia, influenza pneumonia, pleural infection and clinically suspected pneumonia, defined as chest infection treated with antibiotics, were evaluated.

Biochemical changes induced by dominant bacteria in chill-stored silver carp (Hypophthalmichthys molitrix) and GC-IMS identification of volatile organic compounds.

To evaluate the spoilage potential of dominant bacteria (Aeromonas allosaccharophila, Pseudomonas psychrophila, and Shewanella putrefaciens) isolated from spoiled silver carp (Hypophthalmichthys molitrix) fillets, biochemical changes including protein degradation, trichloroacetic acid (TCA)-soluble peptides, total volatile basic nitrogen (TVB-N), biogenic amines, nucleotide catabolism, and volatile organic compounds were examined in single-species inoculated silver carp flesh for 14 days at 4 °C. P. psychrophila exhibited the strongest proteolytic activity, which resulted in the highest concentrations of TCA-soluble peptides and TVB-N.

Insights into body size variation in cetaceans from the evolution of body-size-related genes.

Background: Cetaceans exhibit an exceptionally wide range of body size, yet in this regard, their genetic basis remains poorly explored. In this study, 20 body-size-related genes for which duplication, mutation, or deficiency can cause body size change in mammals were chosen to preliminarily investigate the evolutionary mechanisms underlying the dramatic body size variation in cetaceans.

Results: We successfully sequenced 20 body-size-related genes in six representative species of cetaceans.

Large-conductance calcium-activated potassium channels mediate lipopolysaccharide-induced activation of murine microglia.

Large-conductance calcium-activated potassium (BK) channels are ubiquitously expressed in most cell types where they regulate many cellular, organ, and organismal functions. Although BK currents have been recorded specifically in activated murine and human microglia, it is not yet clear whether and how the function of this channel is related to microglia activation. Here, using patch-clamping, Griess reaction, ELISA, immunocytochemistry, and immunoblotting approaches, we show that specific inhibition of the BK channel with paxilline (10 μm) or siRNA-mediated knockdown of its expression significantly suppresses lipopolysaccharide (LPS)-induced (100 ng/ml) BV-2 and primary mouse microglial cell activation.

Reducing antibiotic prescribing in primary care in England from 2014 to 2017: population-based cohort study.

Objective: To analyse individual-patient electronic health records to evaluate changes in antibiotic (AB) prescribing in England for different age groups, for male and female subjects, and by prescribing indications from 2014 to 2017.

Methods: Data were analysed for 102 general practices in England that contributed data to the UK Clinical Practice Research Datalink (CPRD) from 2014 to 2017. Prescriptions for all ABs and for broad-spectrum β-lactam ABs were evaluated.

Preoperative Blindfold Training Prevents Pediatric Psychological Behavior Disorders During the Anesthesia Recovery Period: A Randomized Controlled Trial.

Purpose: To identify the effectiveness and feasibility of blindfold training on preventing pediatric psychological behavior disorders during the anesthesia recovery period.

Design: This study investigated the effect of blindfold training through the assessment of anxiety, delirium, and pain in children during the anesthesia recovery period.

Methods: This study was a prospective, randomized, controlled trial.

Behavior of Sulfur Oxides in Nonferrous Metal Smelters and Implications on Future Control and Emission Estimation.

This study jointly conducted a field investigation and thermodynamic model simulation in three nonferrous metal smelters (NFMS) to identify sulfur oxides (SOX) formation, transformation, and emissions in the flue gas. Most of SOX was released as sulfur dioxides (SO) at the outlet of the furnace with the molar proportion of sulfur trioxides (SO) of 1.0-4.

Effect of ε-polylysine and ice storage on microbiota composition and quality of Pacific white shrimp (Litopenaeus vannamei) stored at 0 °C.

This study evaluated the effects of ε-Polylysine and ice storage on microbiota composition and quality attributes of Pacific white shrimp stored at 0 °C. The sensorial shelf-life of control, 0.1% ε-Polylysine treated group, and ice stored group were 5, 8, and 7 days, respectively.

Maternal exposure to fipronil results in sulfone metabolite enrichment and transgenerational toxicity in zebrafish offspring: Indication for an overlooked risk in maternal transfer?

Ecotoxicological studies show the association between pesticide pollution and transgenerational toxicity in aquatic organisms. However, a less considered risk is that many pesticides can be metabolized and transferred to offspring as new toxicants. In this study, we used zebrafish to evaluate the maternal transfer risk of fipronil (FIP), which is a great threat to aquatic organisms with toxic metabolite formation.

The treatment role of L. to triple-negative breast cancer cells.

L. is widely used in Traditional Chinese Medicine and studies have reported its anticancer effect, but its chemical composition and therapy mechanism remains unknown. This research aims to analyze the chemical components of the ethanol extract of L.

Pressure-dependent rate rules for cycloaddition, intramolecular H-shift, and concerted elimination reactions of alkenyl peroxy radicals at low temperature.

The reactions of cycloaddition, intramolecular H-shift and concerted elimination of alkenyl peroxy radicals are three kinds of important reactions in the low temperature combustion of alkenes. In this study, the cycloaddition reactions are divided into classes considering endo-cycloaddition, exo-cycloaddition and the size of the transition states; the intramolecular H-shift reactions are divided into classes depending upon the ring size of the transition states and the type of C-H bonds from which the hydrogen atom is transferred; the concerted elimination reactions are divided into classes according to the type of H-CCOO bond that is broken. All geometry optimizations are performed at the B3LYP/6-31G(2df,p) level.

Long-term exposure to the non-steroidal anti-inflammatory drug (NSAID) naproxen causes thyroid disruption in zebrafish at environmentally relevant concentrations.

The presence of trace levels of pharmaceuticals is an emerging issue impacting the aquatic ecosystem. Naproxen (NPX) is a nonsteroidal anti-inflammatory drug (NSAID) that has been frequently detected in aquatic environments worldwide. Recently, concerns regarding endocrine disruption by NSAIDs have increased; however, their effects on the thyroid system have yet to be understood.

Prognosis Prediction of Colorectal Cancer Using Gene Expression Profiles.

Investigation on prognostic markers for colorectal cancer (CRC) deserves efforts, but data from China are scarce. This study aimed to build a prognostic algorithm using differentially expressed gene (DEG) profiles and to compare it with the TNM staging system in their predictive accuracy for CRC prognosis in Chinese patients. DEGs in six paired tumor and corresponding normal tissues were determined using RNA-Sequencing.

Antitumor Effects of Ruyiping on Cell Growth and Metastasis in Breast Cancer.

Ruyiping is an effective traditional Chinese herbal medicine formula for preventing postoperative recurrence and metastasis of breast cancer. However, the exact function and underlying mechanism of Ruyiping in breast cancer remain unclear. After breast cancer cell lines MDA-MB-231 and MDA-MB-468 were treated with Ruyiping, the CCK8, colony formation, wound-healing, and transwell invasion assays were used to examine cell proliferation, migration, and invasion, respectively.

Biosynthetic bifunctional enzyme complex with high-efficiency luciferin-recycling to enhance the bioluminescence imaging.

Firefly luciferase is a prominent reporter on molecular imaging with the advantage of longer wavelength on light emission and the ATP linear correlation, which makes it useful in most of current bioluminescence imaging model. However, the utility of this biomaterial was limited by the signal intensity and stability which are respectively affected by enzyme activity and substrate consumption. This study demonstrated a series of novel synthetic bifunctional enzyme complex of Firefly luciferase (Fluc) and Luciferin-regenerating enzyme (LRE).