Fully automated in vivo screening system for multi-organ imaging and pharmaceutical evaluation.

Advancements in screening technologies employing small organisms have enabled deep profiling of compounds in vivo. However, current strategies for phenotyping of behaving animals, such as zebrafish, typically involve tedious manipulations. Here, we develop and validate a fully automated in vivo screening system (AISS) that integrates microfluidic technology and computer-vision-based control methods to enable rapid evaluation of biological responses of non-anesthetized zebrafish to molecular gradients.

Interspecies ecological competition rejuvenates decayed Geobacter electroactive biofilm.

Bioelectrochemical systems (BESs) exploit electroactive biofilms (EABs) for promising applications in biosensing, wastewater treatment, energy production, and chemical biosynthesis. However, during the operation of BESs, EABs inevitably decay. Seeking approaches to rejuvenate decayed EABs is critical for the sustainability and practical application of BESs.