Dysfunctional lactate metabolism in the brain has been implicated in neuroinflammation, Aβ deposition, and cell disturbance, all of which play a significant role in the pathogenesis of Alzheimer's disease (AD). In this study, we aimed to investigate the lactate metabolism-related genes (LMRGs) in AD via an integrated bulk RNA and single-nuclei RNA sequencing (snRNA-seq) analysis, with a specific focus on microglia. We obtained 26 HC and 24 AD snRNA-seq samples originated from human prefrontal cortex in Gene Expression Omnibus (GEO) database and collected 873 LMRGs from three databases, namely MSigDB, The Human Protein Atlas and GeneCards.
View Article and Find Full Text PDFWith the development of the shipbuilding industry, it is necessary to improve tribological properties of polyether ether ketone (PEEK) as a water-lubricated bearing material. In this study, the sulfonated PEEK (SPEEK) with three distinct chemical structures was synthesized through direct sulfonated polymerization, and high fault tolerance and a controllable sulfonation degree ensured the batch stability. The tribological and mechanical properties of SPEEK with varying side groups (methyl and tert-butyl) and rigid segments (biphenyl) were compared after sintering in a vacuum furnace.
View Article and Find Full Text PDFBackground: This study aimed to explore the association of the second birth delivery mode and interval with maternal pelvic floor changes.
Methods: This prospective cohort study included women who had a first delivery and were in weeks 36-41 of a subsequent pregnancy at Panzhihua Central Hospital between July 2017 and June 2018. The primary outcomes of the study were the hiatus area at 6 months postpartum and bladder neck (mm) at rest and during a maximum Valsalva maneuver.
Context: Liuwei Dihuang pill (LWDH) has been used to treat postmenopausal osteoporosis (PMOP).
Objective: To explore the effects and mechanisms of action of LWDH in PMOP.
Materials And Methods: Forty-eight female Sprague-Dawley rats were divided into four groups: sham-operated (SHAM), ovariectomized (OVX), LWDH high dose (LWDH-H, 1.
Objective: Epigenetic abnormalities have a critical role in breast cancer by regulating gene expression; however, the intricate interrelationships and key roles of approximately 400 epigenetic regulators in breast cancer remain elusive. It is important to decipher the comprehensive epigenetic regulatory network in breast cancer cells to identify master epigenetic regulators and potential therapeutic targets.
Methods: We employed high-throughput sequencing-based high-throughput screening (HTS) to effectively detect changes in the expression of 2,986 genes following the knockdown of 400 epigenetic regulators.
Objective: Pelvic floor dysfunction (PFD) is highly prevalent among women. Pelvic floor ultrasound (PFU) is a critical method for assessing PFD. This study examined the knowledge, attitudes, and practice (KAP) of women of childbearing age regarding PFD and PFU.
View Article and Find Full Text PDFBiodegradable polylactide (PLA) has been widely utilized in people's daily lives. In order to improve the fire safety of PLA, ammonium polyphosphate (APP) was self-assembled onto the surface of serpentine NiSiO(OH) through the electrostatic method, followed by mixing with PLA by melt compounding. The APP-modified serpentine (serpentine@APP) dispersed uniformly in the PLA matrix.
View Article and Find Full Text PDFThermoplastic polyurethane (TPU) is widely used in daily life due to its characteristics of light weight, high impact strength, and compression resistance. However, TPU products are extremely flammable and will generate toxic fumes under fire attack, threatening human life and safety. In this article, a nanohybrid flame retardant was designed for the fire safety of TPU.
View Article and Find Full Text PDFObjective: Acute lung injury (ALI) is a common complication of heat stroke (HS) and a direct cause of death. However, the mechanism underlying ALI following HS remains unclear.
Method: To investigate whether ferroptosis is involved in HS-ALI.
Design of high-efficiency visible light photocatalysts is critical in the degradation of antibiotic pollutants in water, a key step towards environmental remediation. In the present study, Mo-doped BiOBr nanocomposites are prepared hydrothermally at different feed ratios, and display remarkable visible light photocatalytic activity towards the degradation of sulfanilamide, a common antibacterial drug. Among the series, the sample with 2% Mo dopants exhibits the best photocatalytic activity, with a performance 2.
View Article and Find Full Text PDFTranscriptional reprogramming contributes to the progression and recurrence of cancer. However, the poorly elucidated mechanisms of transcriptional reprogramming in tumors make the development of effective drugs difficult, and gene expression signature is helpful for connecting genetic information and pharmacologic treatment. So far, there are two gene-expression signature-based high-throughput drug discovery approaches: L1000, which measures the mRNA transcript abundance of 978 "landmark" genes, and high-throughput sequencing-based high-throughput screening (HTS); they are suitable for anticancer drug discovery by targeting transcriptional reprogramming.
View Article and Find Full Text PDFMagnetic FeS was facilely derived from MIL-100(Fe) as the precursor and thioacetamide (TAA) as the sulfur source under hydrothermal condition. The as-prepared FeS was adopted as catalyst to promote the photo-Fenton process, in which sulfamethoxazole (SMX) was used as representative pollutant sample to test the oxidative degradation performance of FeS. The results showed that FeS exhibited excellent photo-Fenton-like oxidation decomposition performances toward sulfamethoxazole (SMX) under both UV and visible light.
View Article and Find Full Text PDFHydroxyl modified UiO-66 ((OH)-UiO-66-X%, X represents the mass content ratio of introduced 2,5-dihydroxyterephthalic acid) was prepared via a solvothermal reaction between zirconium tetrachloride, benzene-1,4-dicarboxylic acid (HBDC), as well as 2,5-dihydroxyterephthalic acid (HBDC-(OH)). It was found that hydroxyl groups can act as the intramolecular hole scavenger to boost the photo-induced charge carrier separation to enhance Cr(VI) reduction. The photocatalytic Cr(VI) reduction activities of (OH)-UiO-66-X% were investigated upon the irradiation of low-power ultraviolet LED light.
View Article and Find Full Text PDFElectromyographic biofeedback (EMG-BF) can be regarded as an adjuvant to pelvic floor muscle (PFM) training (PFMT) for the management of stress urinary incontinence (SUI). This meta-analysis aimed to compare the efficacy of PFMT with and without EMG-BF on the cure and improvement rate, PFM strength, urinary incontinence score, and quality of sexual life for the treatment of SUI or pelvic floor dysfunction (PFD). PubMed, EMBASE, the Cochrane Library, Web of Science, Wanfang, and CNKI were systematically searched for studies published up to January 2021.
View Article and Find Full Text PDFSeries of MIL-100(Fe)/CoS composites (MxCy) were facilely fabricated using ball-milling method. The optimum M50C50 exhibited extremely higher Fenton-like catalytic degradation activity toward bisphenol A (BPA) than the pristine MIL-100(Fe) and CoS. The significant improvement of BPA degradation was attributed to the synergetic effect between MIL-100(Fe) and CoS with the synergistic factor being 95.
View Article and Find Full Text PDFThe Z-scheme MIL-100(Fe)/PANI composite photocatalysts were facilely prepared from MIL-100(Fe) and polyaniline (PANI) by ball-milling, and were characterized by powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), transmission electron microscopy (TEM), UV-visible diffuse-reflectance spectrometry (UV-vis DRS), X-ray photoelectron spectroscopy (XPS) and photoluminescence emission spectrometry (PL). The photocatalytic activities of MIL-100(Fe)/PANI composites were investigated via tetracycline degradation and hexavalent chromium reduction in aqueous solution under the irradiation of white light. The results revealed that the MIL-100(Fe)/PANI composite photocatalysts exhibited outstanding photocatalytic activities toward Cr(VI) reduction and tetracycline decomposition.
View Article and Find Full Text PDFThe purpose of the present study was to investigate the effect of bufadienolides on the A‑375 melanoma cell line, and to delineate the underlying mechanism. A Cell Counting Kit‑8 assay was used to determine the viability of the cells, and flow cytometry was used to evaluate apoptosis. Western blot analysis was used to evaluate the expression levels of proteins involved in the AKT pathway that are associated with apoptosis and autophagy.
View Article and Find Full Text PDFIn this study, a series of BUC-21/titanate nanotube (BT-X) composites were facilely fabricated via ball-milling of 2-dimensional (2D) metal-organic framework (MOF) BUC-21 and titanate nanotubes (TNTs). The BT-X composites were characterized by powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), UV-visible diffuse-reflectance spectroscopy (UV-vis DRS), X-ray photoelectron spectrometer (XPS) and high resolution transmission electron microscopy (HRTEM). Both the photocatalytic reduction from Cr(VI) to Cr(III) and adsorptive removal of formed Cr(III) of BT-X composites were systematically investigated under different conditions including pH values and co-existing inorganic ions.
View Article and Find Full Text PDFBiosci Biotechnol Biochem
December 2016
Alzheimer's disease (AD) is the most common cause of dementia. Its pathology often accompanies inflammatory action, and astrocytes play important roles in such procedure. Rela(p65) is one of significant message factors in NF-κB pathway which has been reported high expression in astrocyte treated by Aβ.
View Article and Find Full Text PDFOptical bistable behaviors induced by tunneling coupling are demonstrated in an asymmetric double quantum-well structure including a unidirectional ring cavity. We analytically find that optical bistability can be obtained with the existence of resonant tunneling. Furthermore, the threshold of the optical bistability can be modulated by the intensity or the detuning of the driving fields.
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