Influence of endoscopic sphincterotomy on hepatolithiasis with Oddi sphincter-preserved cholangioplasty with hepatico-subcutaneous stoma: A three-decade, real word cohort study.

Background: Hepatolithiasis frequently presents with recurring cholangitis and complications. Oddi sphincter-preserved cholangioplasty with hepatico-subcutaneous stoma (OSPCHS), introduced in 1993, has shown favorable long-term results. Endoscopic sphincterotomy (EST) is commonly used, but its impact on OSPCHS outcomes remains unclear.

Using Sediment Bacterial Communities to Predict Trace Metal Pollution Risk in Coastal Environment Management: Feasibility, Reliability, and Practicability.

The distribution of trace metals (TMs) in a continuous water body often exhibits watershed attributes, but the tidal gates of the coastal rivers may alter their transformation and accumulation patterns. Therefore, a tidal gate-controlled coastal river was selected to test the distribution and accumulation risks of Al, As, Cr, Cu, Fe, Mn, Ni, Sr, and Zn in the catchment area (CA), estuarine area (EA), and offshore area (OA). Associations between TMs and bacterial communities were analyzed to assess the feasibility of using bacterial parameters as ecological indicators.

Diversity and Isolation of Endophytic Fungi in and Biotransformation Activity on Saponins.

Objective: This study reports the diversity and community structure differences of the endophytic fungi of of different ages to obtain novel endophytic fungi with glycoside hydrolytic activity for rare saponins production.

Methods: This study used the high-throughput sequencing method to analyze the diversity and community structure of endophytic fungi of . The endophytic fungi were processed by traditional isolation, culture, conservation, and ITS rDNA sequence analyses.

Long-term outcomes of surgical approaches for bilateral hepatolithiasis: A real-world six-decade experience at a single center.

Background: Bilateral hepatolithiasis is an intractable disease and repeated attacks of acute cholangitis seriously threaten patient health. The surgical approaches evolve along with gradually greater understanding of its pathophysiology.

Methods: This is a retrospective cohort study for bilateral hepatolithiasis from January 1958 to December 2018.

Role of osteopontin in diet-induced brown gallstone formation in rats.

Background: Although osteopontin (OPN) is expressed in the liver and pigment gallstones of patients with hepatolithiasis, its role in pigment gallstone formation remains unclear. This study aimed to explore the function of OPN in pigment gallstone formation.

Methods: Rats were fed a chow diet (CD) or lithogenic diet (LD) for 10 consecutive weeks; blocking tests were then performed using an OPN antibody (OPN-Ab).

Risk factors and management of primary choledocholithiasis: a systematic review.

Background: Primary choledocholithiasis (PC) is a common disease in biliary surgery. The treatment is always challenging due to its high recurrence. A systemic review is undertaken to determine the risk factors for recurrence and provide with the individualized management strategy.

Tumour radiological appearance evaluated by enhanced CT correlates with tumour progression and survival in curable gallbladder cancer.

Background: Surgical resection has been proposed for curable gallbladder cancer (GBCA); however, optimal preoperative evaluation and resection planning methods remain unestablished. The aim of this study was to establish the types of CT tumour radiological appearances in GBCA with a focus on its association with clinicopathologic features and its prognostic impact in curable GBCA.

Methods: In all, 118 patients surgically treated for GBCA were identified and CT tumour radiological appearances were reviewed.

Distribution and diversity of endophytic fungi in and cytotoxic activities.

Objective: In the present study, was screened for fungi communities to clarify their diversity and community assemblage in hosts. Meanwhile, the identification and activity assays of the strains were also conducted.

Methods: By culture-dependent (endophytic fungi isolations from plant sections) and culture-independent (metagenomic library and cloning from plant sections) techniques, fungi communities were studied.

Sample-Sample Correlation Asynchronous Spectroscopic Method Coupled with Multivariate Curve Resolution-Alternating Least Squares To Analyze Challenging Bilinear Data.

An approach to construct a secondary asynchronous spectrum via sample-sample correlation (SASS) is proposed to analyze bilinear data from hyphenated spectroscopic experiments. In SASS, bilinear data is used to construct a series of two-dimensional (2D) sample-sample correlation spectra. Then a vertical slice is extracted from each 2D sample-sample correlation spectrum so that a secondary 2D asynchronous spectrum is constructed via these slices.

The Advancement of Long Non-Coding RNAs in Cholangiocarcinoma Development.

Cholangiocarcinoma (CCA) is a malignancy with increasing incidence in recent years. CCA patients are usually diagnosed at advanced stage due to lack of apparent symptoms and specifically diagnostic markers. Nowadays, surgical removal is the only effective method for CCA whereas overall 5-year-survival rate keeps around 10%.

Laparoscopic treatment for suspected gallbladder cancer confined to the wall: a 10-year study from a single institution.

Objective: Although laparoscopic treatment of gallbladder cancer (GBC) has been explored in the last decade, long-term results are still rare. This study evaluates long-term results of intended laparoscopic treatment for suspected GBC confined to the gallbladder wall, based on our experience over 10 years.

Methods: Between August 2006 and December 2015, 164 patients with suspected GBC confined to the wall were enrolled in the protocol for laparoscopic surgery.

Analysis of Characteristic Parameters of the Surface Fourier Transform Infrared Spectra from Normal Human Breast.

A total of two hundred cases of surface Fourier transform infrared (FTIR) spectra from normal female breast were obtained by scanning two hundred enrolled healthy volunteers’ upper limit of the right breast outside the skin with two mid-infrared optical fibers and an attenuated total reflectance (ATR) probe. Twelve characteristic bands of the above FTIR spectra, related to deoxyribonucleic acid, ribonucleic acid, proteins, lipids and carbohydrate were analyzed. Thirty-six spectral parameters of these characteristic bands, including peak positions (P), intensities (I) and full width at half maximum (F) of FTIR spectrums, were statistically analyzed.

Characteristics Analysis of the Thyroid Gland Surface of Normal Human with Fourier Transform Infrared (FTIR) Spectra.

In order to obtain 240 cases of the surface Fourier transform infrared (FTIR) spectra of normal human thyroid gland, 120 healthy volunteers were enrolled in this study and scanned by using two mid-infrared optical fibers equipped with an attenuated total reflectance (ATR) to probe through the skin of two lobes of thyroid gland. Then whether there were differences between the surface FTIR spectra of the right lobe and that of the left part of the normal human thyroid gland was explored by comparing the 35 variables of 12 bands, including peak positions, relative intensity ratios and full width at half maximum of FTIR spectrums. Also the spectra characteristics of unique absorbent bands that represent changes of structure and quantity of variance biochemical substances such as nucleic acids, proteins and carbohydrates were illustrated.

Oddi sphincter preserved cholangioplasty with hepatico-subcutaneous stoma for hepatolithiasis.

Aim: To evaluate the long-term outcomes of Oddi sphincter preserved cholangioplasty with hepatico-subcutaneous stoma (OSPCHS) and risk factors for recurrence in hepatolithiasis.

Methods: From March 1993 to December 2012, 202 consecutive patients with hepatolithiasis underwent OSPCHS at our department. The Oddi sphincter preserved procedure consisted of common hepatic duct exploration, stone extraction, hilar bile duct plasty, establishment of subcutaneous stoma to the bile duct.

[Influence of Concentration Sequence on the Signal-to-Noise Ratio of Cross Peaks of 2D Asynchronous Spectra Generated by Using the DAOSD Approach].

In the present work, computer simulation was performed on a model chemical system where two solutes (denoted as P and Q, respectively) are dissolved in the same solution. Under intermolecular interaction between P and Q, part of P undergoes subtle structural variation and converts into U while part of Q converts into V. The strength of intermolecular interaction can be characterized by the corresponding equilibrium constant K.

[Research on Application of Fourier Transform Infrared Spectrometry in the Diagnosis of Lymph Node Metastasis in Gastric Cancer].

To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of lymph node metastasis in gastric cancer by Fourier transform infrared (FTIR) spectrometry. FTIR spectra of surgically removed fresh lymph nodes were measured by FTIR via probe of attenuated total reflection (ATR). For each spectrum, 13 bands were indentified and assigned between 3 000 and 1 000 cm(-1).

Laparoscopic hepaticoplasty using gallbladder as a subcutaneous tunnel for hepatolithiasis.

Aim: To investigate the feasibility, efficacy and safety of laparoscopic hepaticoplasty using gallbladder as subcutaneous tunnel and sphincter-of-Oddi preservation for hepatolithiasis.

Methods: From January 2010 to July 2013, six patients with hepatolithiasis were treated at our institution. All the patients underwent laparoscopic surgery.

Detection of gastric cancer with Fourier transform infrared spectroscopy and support vector machine classification.

Early diagnosis and early medical treatments are the keys to save the patients' lives and improve the living quality. Fourier transform infrared (FT-IR) spectroscopy can distinguish malignant from normal tissues at the molecular level. In this paper, programs were made with pattern recognition method to classify unknown samples.

Synthesis of nanoscale zero-valent iron/ordered mesoporous carbon for adsorption and synergistic reduction of nitrobenzene.

Nanoscale zero-valent iron (NZVI) supported on ordered mesoporous carbon (OMC) was synthesized through liquid phase reduction route. The NZVI/OMC composite was characterized by X-ray diffraction, N(2) adsorption/desorption and transmission electron microscopy. Results reveal that the composite possesses ordered mesostructure with NZVI distributing homogeneously on the surface of OMC support.

Strategies for diagnosis of xanthogranulomatous cholecystitis masquerading as gallbladder cancer.

Background: Patients with xanthogranulomatous cholecystitis sometimes exhibit imaging and intraoperative findings that are similar to those of advanced gallbladder cancer, thus these patients are easily misdiagnosed. The present study aimed to investigate the characteristics of xanthogranulomatous cholecystitis masquerading as gallbladder cancer that could potentially aid in the correct diagnosis of this condition.

Methods: The clinical, serological, radiological and operative features of twelve patients with obviously wall-thickening or mass-forming xanthogranulomatous cholecystitis were retrospectively analyzed.

HIV-1 Tat increases oxidant burden in the lungs of transgenic mice.

Chronic human immunodeficiency virus infection is associated with higher incidence of pulmonary complications including hypertension, vasculopathy, lymphocytic alveolitis, and interstitial pneumonitis not attributed to either opportunistic infections or presence of the virus. The Tat (transactivator of transcription) protein, a required transactivator for expression of full-length viral genes, is pleiotropic and influences expression of cellular inflammatory genes. Tat-dependent transactivation of cellular genes requires specific mediators, including NF-κB, widely recognized as sensitive to changes in cellular oxidant burden.

[Treatment of hepatolithiasis by multiple operative methods with hepatico-subcutaneous stoma].

Objective: To evaluate the mid-term and long-term outcomes of patients with hepatolithiasis after receiving multiple operative methods with hepatico-subcutaneous stoma.

Methods: A retrospective analysis was performed based on 156 patients with hepatolithiasis who underwent multiple operative methods with hepatico-subcutaneous stoma from Apr. 1993 to Mar.

An end-to-end anastomosis model of guinea pig bile duct: A 6-mo observation.

Aim: To establish the end-to-end anastomosis (EEA) model of guinea pig bile duct and evaluate the healing process of bile duct.

Methods: Thirty-two male guinea pigs were randomly divided into control group, 2-, 3-, and 6-mo groups after establishment of EEA model. Histological, immunohistochemical and serologic tests as well as measurement of bile contents were performed.

[Fourier transform infrared spectroscopic study on gallbladder carcinoma].

Fourier transform infrared (FTIR) spectroscopy with an attenuated total reflection(ATR) probe was used to study the benign and malignant tissues of gallbladder. FTIR spectra of 18 tissues of gallbladder carcinoma and 139 benign tissues were collected and analyzed. The results demonstrated that bands related to lipid, carbohydrate, and nucleic acid from malignant samples were significantly different from those of benign ones: (1) The peak positions of 1 167 and 1 123 cm(-1) shifted toward lower wave number (P < 0.

Intraoperative detection of thyroid carcinoma by fourier transform infrared spectrometry.

Background: Fourier transform infrared (FTIR) spectroscopy is a powerful tool for distinguishing cancerous tissue from normal one. Our aim in this study was to establish tissue discriminant analysis for thyroid malignancy and benign samples intraoperatively using FTIR spectroscopy.

Methods: Seventeen papillary thyroid cancer and 43 nodular goiter tissues were obtained and underwent FTIR spectroscopy scanning intraoperatively.