Publications by authors named "Xiaofei Li"

The clinical application of cantharidin (CTD) is seriously limited due to its nephrotoxicity. Therefore, this study aims to investigate sensitive biomarkers for the evaluation and prediction of nephrotoxicity induced by CTD in rat. A total of 80 rats were randomly divided into four groups: control group and three doses of CTD groups.

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Cardiac troponin I (cTnI) is known to be among the prominent diagnostic bio-marker for acute myocardial infarction (AMI). In this paper, we proposed an electrochemical aptasensor with nanomaterial MoS/CuS-Au as the substrate material and perillaldehyde (PA) as the surface-initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization monomer by mediating the in-situ deposition of silver metal on the electrode surface for ultrasensitive detection of cTnI. The substrate material MoS/CuS-Au not only accelerated the rate transfer between electron, but also provided more active sites for aptamers introduction.

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Objective: Bone resorption mediated by osteoclast differentiation induces the occurrence of bone-related diseases. Macrophages, an origin of osteoclasts, whose M2 type can reduce inflammation-induced bone damage. We aimed to investigate the effect of M2 macrophage-derived exosomes on osteoclast formation and elucidate its underlying mechanism.

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Objective: This study aimed to explore the repair effect of siRNA-mediated double silencing of the mechanically sensitive ion channels Piezo1 and TRPV4 proteins on a rat model of osteoarthritis.

Methods: Piezo1 and TRPV4 interference plasmids were constructed using siRNA technology. Sprague Dawley (SD) rats were divided into four groups: the model group, siRNA-Piezo1, siRNA-TRPV4, and double gene silencing groups.

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Drought has been found to affect the size and color of precious heartwood of Dalbergia odorifera, but the mechanism remains unclear. For this purpose, we performed the measurement of heartwood size, color, and flavonoid content and composition in a 15-year-old mixed plantation of D. odorifera and Santalum album that had been subjected to two levels of rainfall exclusion and control treatments for seven years, and carbon isotope labeling and anatomical observation in 2-year-old potted D.

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  • Adeno-associated virus serotype 8 (AAV8) is a promising vector for gene therapy, but its purification is difficult due to low abundance and strict purity needs.
  • This study developed a nanobody (Nb)-based affinity chromatography resin, identifying Nb9 as the best candidate for effectively purifying AAV8 with high binding affinity and yield.
  • The purification method showed excellent specificity and consistency across multiple cycles, suggesting it could be a scalable and cost-effective solution for AAV8 purification and highlighting the potential of Nbs in biotechnology.
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Biochar is extensively utilized for the remediation of environments contaminated with heavy metals (HMs). However, its derived-dissolved organic matter (BDOM) can interact with iron oxides, which may adversely influence the retention of HMs. This study investigates the effect of BDOM derived from tobacco stalk (TS) and tobacco petiole (TP) biochar on the redistribution behavior of As(V) in acid mine drainage (AMD)-impacted environments, particularly concentrating on the interactions with Schwertmannite (Sch).

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Radiotherapy sensitivity is associated with the prognosis of patients with tongue squamous cell carcinoma (TSCC). In the present study, we proposed to explore the specific mechanism of interventional radiology (IR) therapy for TSCC in vitro and in vivo. TSCC cells were treated with 6 Gy IR and tumor bearing mice were treated with 20 Gy × 1 IR.

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  • * The researchers developed a new method called TRISCO, which helps retain RNA signals in cleared organs for better imaging, specifically for whole-brain 3D RNA visualization.
  • * TRISCO effectively preserves RNA integrity, ensures uniform labeling, and enhances tissue transparency, making it a powerful tool for detailed transcriptional spatial analysis at the single-cell level across various organs.
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Ménière's disease (MD) is a complex inner ear disorder characterized by a range of symptoms, with its pathogenesis linked to immune-related mechanisms. Our previous research demonstrated that IL-1β maturation and release can trigger cell pyroptosis, exacerbating the severity of the endolymphatic hydrops in a mouse model; however, the specific mechanism through which IL-1β influences MD symptoms remains unclear. This study conducted on patients with MD examined changes in protein signatures in the vestibular end organs (VO) and endolymphatic sac (ES) using mass spectrometry.

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In the heart, cell-matrix and cell-cell adhesions reorganize in response to increased cardiac demand and growth to promote cardiomyocyte maturation. Vinculin, a mechanosensitive adaptor protein, links filamentous actin to cell-matrix and cell-cell adhesions and is thus positioned to regulate adhesion reorganization. However, how the two adhesion systems are coordinated in the heart, and the role of vinculin in this process is poorly understood.

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The hepatotoxicity mechanism of cantharidin (CTD), a major active component of Mylabris was explored based on liver lipidome alterations and spatial distributions in female and male rats using lipidomics and matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). After oral CTD exposure, the livers of female rats were screened for 104 differential lipids including lysophosphatidylethanolamine(LysoPE)(20:2/0:0) and diacylglycerol(DG)(18:2/22:4), whereas the livers of male rats were screened for 76 differential lipids including fatty acid(FA)(24:6) and DG(18:0/22:4). According to the MALDI-MSI results, female rats exhibited 12 differential lipids with alteration in the abundance and spatial distribution of phosphatylcholine(PC), phosphatidylethanolamine(PE), lysophosphatidylcholine(LysoPC), and LysoPE in the liver lesion area.

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Cisplatin (CP) is a widely utilized anticancer drug, which also produces significant side effects, notably acute kidney injury (AKI). Fermented Eucommia ulmoides leaf (FEUL), a medicinal and edible Chinese herbal remedy, is known for its renoprotective properties. However, the effect and underlying mechanism of FEUL extract in AKI therapy have remained largely unexplored.

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Background: Epidemiological studies show inconsistent links between hearing/vision impairment and dementia risk. Using multisource data, we investigated how single or combined sensory impairments relate to risks of all-cause and specific types of dementia.

Methods: We employed a triangulation approach combining three methodologies.

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Article Synopsis
  • The study focuses on the lung's development during the first trimester of human pregnancy, emphasizing its importance for life on land.
  • It utilizes advanced imaging and analysis techniques to map the protein-level organization of lung cells over various stages of gestation, examining more than 2 million cells.
  • The findings offer a detailed resource on the types of cells in the developing lung as well as their growth and spatial patterns, serving as a foundation for future research on respiratory health.
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  • Lepidocrocite, an iron oxide, is important for how heavy metals interact and change in soil and sediment, but how it behaves over time, especially with chromium (Cr(VI)), isn't fully understood.
  • * The study shows that the transformation of lepidocrocite into other forms like goethite and hematite depends on its shape and exposed facets, with rod-like lepidocrocite being more stable compared to plate-like lepidocrocite.
  • * Cr(VI) binding boosts lepidocrocite's stability, particularly on certain facets, and affects its release rates; for instance, Cr(VI) was released more from plate-like lepidocrocite than from rod-like lepidoc
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Magnetic adsorbents can effectively remove heavy metals from soil. However, the magnetization process may reduce availability of adsorption sites, making it challenging to balance magnetic and adsorption properties. In this study, oyster shell (OS) and clam shell (CS) material was magnetized by an improved chemical co-precipitation method.

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Objectives: This study explored the expression and diagnostic value of differentially expressed miR-3591-5p in congenital heart disease-associated pulmonary arterial hypertension (CHD-PAH).

Methods: A total of 110 CHD patients were divided into four groups based on their mean pulmonary artery pressure (PAPm). The plasma miR-3591-5p expression was determined by reverse transcription polymerase chain reaction.

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Background: Few studies have focused on the region-specific relationship between cardiovascular disease (CVD) and low temperature worldwide.

Objective: We aimed to provide an overview of trends in mortality and disability-adjusted life years (DALYs) for CVD and its subtypes attributable to low temperature over the past 30 years in 204 countries and regions, along with the associations of these trends with age, period, and birth cohorts.

Methods: Data on the estimated burden of CVDs (including ischemic heart disease, hypertensive heart disease, and stroke) attributable to low temperature were obtained from the Global Burden of Disease Study 2019.

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  • This study conducted a network meta-analysis to evaluate the effectiveness of different exercise interventions on the quality of life (QoL) for women post-breast cancer surgery.
  • Research data was collected from 8 databases and analyzed using specific statistical tools to ensure quality and reliability.
  • Results indicated that combined aerobic and resistance exercises were the most effective, with varying impacts of other interventions like yoga, tai chi, and walking on different QoL outcome measures.
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  • Systemic immune-mediated diseases (SIDs) may contribute to dilated cardiomyopathy (DCM), and this study aimed to explore the genetic predispositions present in DCM patients with SIDs.
  • The research involved 183 DCM-SID patients, identifying a significantly higher prevalence of pathogenic genetic variants in these individuals compared to healthy controls and DCM patients without SIDs.
  • Findings suggest that about 17-20% of DCM patients with SIDs have pathogenic variants, particularly truncating variants like TTN, indicating the importance of genetic testing for understanding the causes of immune-related DCM.
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  • Large mammalian herbivores (LMH), like domestic cattle, play a crucial role in grassland ecosystems by influencing biodiversity and food-web dynamics.
  • A study in northern China found that the presence of cattle caused grasshoppers to jump significantly more, leading to an increased foraging response from their bird predator, the barn swallow, while also reducing grasshopper populations by approximately 50%.
  • The research revealed that while jumping helps grasshoppers avoid being trampled by cattle, it also dramatically increases their vulnerability to being eaten by swallows, highlighting complex interactions between species that affect ecosystem dynamics.
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Objective: To detect the differentially expressed regulatory miRNAs in the late stage of red blood cell (RBC) preservation and predict their roles.

Methods: Suspended RBCs with different storage periods of 35 day, 42 day, and 50 day were collected for routine blood tests, RNA extraction, and preparation of small RNA sequencing libraries. The constructed libraries were sequenced and the biological functions of differential miRNAs in RBCs in the late storage were analyzed by bioinformatics.

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