Transcriptional insights of citrus defense response against Diaporthe citri.

Citrus melanose, caused by Diaporthe citri, is one of the most important and widespread fungal diseases of citrus. Previous studies demonstrated that the citrus host was able to trigger the defense response to restrict the spread of D. citri.

Causing Leaf Spot Disease on × Ramat and Screening of Its Potential Antagonistic Bacteria.

× Ramat. is a famous perennial herb with medicinal, edible, and ornamental purposes, but the occurrence of plant diseases can reduce its value. A serious disease that caused leaf spots in appeared in 2022 in Tongxiang City, Zhejiang Province, China.

The Genome Sequence of the Citrus Melanose Pathogen and Two Citrus-Related Species.

Melanose disease is one the most widely distributed and economically important fungal diseases of citrus worldwide. The causative agent is the filamentous fungus (syn. ).

[Therapeutic mechanism of bleomycin A5 on infancy hemangioma: an experimental study].

Objective: To investigate the therapeutic mechanism of Bleomycin A5 on infancy hemangioma.

Methods: After intralesional injection of Bleomycin A5 into the tumor of animal model of infancy hemangioma, the variation of tumor form was and the variation of tumor structure were observed using light microscope and electron microscope, the variation of tumor gene expression spectra was also tested by DNA microarray technique.

Results: After treatment, the tumor gradually shrunk, hardened, disappeared one month later.

[Effect of bleomycin A5 on the hemangioma-derived endothelial cell line XTPS-1].

Objective: To investigate the effect of bleomycin A5 on the hemangioma-derived endothelial cell line XTPS-1.

Methods: Hemangioma-derived endothelial cell line XTPS-1 was cultured with different concentration of bleomycin A5 (1000, 100, 10, 1, 0 mg/L), and then the survival rate was measured by methyl thiazolyl terazolium (MTT), the variation of cell morphology was observed using inverted phase contrast microscope and electron microscope, the variation of cell cycle and apoptosis rate were measured using flow cytometry.

Results: After 24 hours culture the cell survival rate was (92.

[Establishment of human infancy hemangioma-derived endothelial cell line XPTS-1 and animal model of human infancy hemangioma].

Objective: To establish an immortalized human infancy hemangioma-derived endothelial cell line (HemEC) and animal model of human infancy hemangioma.

Methods: Hemangioma-derived endothelial cells from specimen of human infancy hemangioma were cultured in vitro and monocloed, and then its growth curve was made, karyomorphism of chromosome analyzed, morphologic characteristics observe, factor VIII related antigen identified by immunohistochemical method.Vascular endothelial growth factor receptor 2 (VEGFR-2) was detected by flow cytometry.