Optimization of the production conditions of tri-GOS and lactosucrose from lactose and sucrose with recombinant -galactosidase.

Few studies expressed the β-galactosidase encoding gene from in so far. In the present study, the recombinant β-galactosidase from FMNP01 was used as a catalyst in transgalactosylation to form tri-GOS and lactosucrose. In the presence of lactose and sucrose, six transfer products were formed in the transgalactosylation reaction with recombinant β-galactosidase L.