Arid desert regions are among the harshest ecological environments on Earth. Halophytes, with their unique physiological characteristics and adaptability, have become the dominant vegetation in these areas. Currently, research on halophytes in this region is relatively limited, particularly concerning studies related to their root endophytic fungi, which have been rarely reported on.
View Article and Find Full Text PDFThe present study aimed to extract and purify the glycoprotein from Cirsii Herba (CHPs), and investigate its immunomodulatory activity and molecular mechanism in RAW264.7 macrophages. The results showed that CHPs contained 14.
View Article and Find Full Text PDFRapid detection of corn moisture content(MC) during maturity is of great significance for field cultivation, mechanical harvesting, storage, and transportation management. However, cumbersome operation, time-consuming and labor-intensive operation were the bottleneck in the traditional drying process and dielectric parameter method. Thus, to overcome the above problems, a rapid detection method for corn MC based on improved complete ensemble empirical mode decomposition with adaptive noise (ICEEMDAN) combined with temporal convolutional network-bidirectional gated recurrent unit (TCN-BiGRU) model.
View Article and Find Full Text PDFOrexins are a family of neuropeptides secreted by neurons in the lateral hypothalamus (LH). These peptides act widespreadly across the body by interacting with specific orexin receptors on target cells, which comprise the orexinergic system. Emerging evidence has revealed that the orexinergic system is tightly associated with neuropsychiatric disorders; however, the underlying mechanisms require further exploration.
View Article and Find Full Text PDFEvaluating the role of antimicrobials biotransformation in the regulation of metabolic functions and antimicrobial resistance evolution in wastewater biotreatment systems is crucial to ensuring water security. However, the associated mechanisms remain poorly understood. Here, we investigate triclocarban (TCC, one of the typical antimicrobials) biotransformation mechanisms and the dynamic evolution of systemic function disturbance and antimicrobial resistance risk in a complex anaerobic hydrolytic acidification (HA)-anoxic (ANO)/oxic (O) process.
View Article and Find Full Text PDFBackground: The loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc) is a major pathological hallmark of Parkinson's disease (PD). Orexin B (OXB) has been reported to promote the growth of DA neurons. However, the roles of OXB in the degeneration of DA neurons still remained not fully clear.
View Article and Find Full Text PDFThe horizontal transfer of plasmids has been recognized as one of the key drivers for the worldwide spread of antimicrobial resistance (AMR) across bacterial pathogens. However, knowledge remain limited about the contribution made by environmental stress on the evolution of bacterial AMR by modulating horizontal acquisition of AMR plasmids and other mobile genetic elements. Here we combined experimental evolution, whole genome sequencing, reverse genetic engineering, and transcriptomics to examine if the evolution of chromosomal AMR to triclosan (TCS) disinfectant has correlated effects on modulating bacterial pathogen (Klebsiella pneumoniae) permissiveness to AMR plasmids and phage susceptibility.
View Article and Find Full Text PDFRNA-binding proteins (RBPs)-RNA networks have contributed to cancer development. Circular RNAs (circRNAs) are considered as protein recruiters; nevertheless, the patterns of circRNA-protein interactions in colorectal cancer (CRC) are still lacking. Processing bodies (PBs) formed through liquid-liquid phase separation (LLPS) are membrane-less organelles (MLOs) consisting of RBPs and RNA.
View Article and Find Full Text PDFThe Tibetan Plateau (TP) is essential in modulating climate change in downstream Eastern China (EC). As a meteorology-sensitive pollutant, changes in ozone (O) in connection with the TP have received limited attention. In this study, using climate analysis of the China High Air Pollutants O product and ERA5 reanalysis data of meteorology for 1980-2020, the effect of springtime TP thermal forcing on the warm season (April-September) O pollution over EC was investigated.
View Article and Find Full Text PDFAims: This investigation aims to elucidate the mechanism underlying sorafenib-induced ferroptosis in hepatocellular carcinoma (HCC).
Methods: The role of dual specificity phosphatase 4 (DUSP4) in sorafenib-treated HCC was investigated using comprehensive assessments both in vitro and in vivo, including Western blotting, qRT-PCR, cell viability assay, lipid reactive oxygen species (ROS) assay, immunohistochemistry, and xenograft tumor mouse model. Additionally, label-free quantitative proteomics was employed to identify potential proteins associated with DUSP4.
Rhizosphere soil fungal and enzyme activities affect the nutrient cycling of terrestrial ecosystems, and rhizosphere fungi are also important participants in the ecological process of vegetation succession, responding to changes in plant communities. Stipa is an excellent forage grass with important ecological and economic value, and has the spatial distribution pattern of floristic geographical substitution. In order to systematically investigate the synergistic response strategies of fungal communities and enzyme activities in the rhizosphere under the vegetation succession.
View Article and Find Full Text PDFBackground: The global spread of plasmid-borne carbapenem resistance is an ongoing public health challenge; however, the nature of such horizontal gene transfer events among complex bacterial communities remains poorly understood. We examined the in-situ transfer of the globally dominant New Delhi metallo-β-lactamase (NDM)-5-positive IncX3 plasmid (denoted pX3_NDM-5) in hospital wastewater to simulate a real-world, One Health antimicrobial resistance context.
Methods: For this transmission study, we tagged pX3_NDM-5 with the green fluorescent protein gene, gfp, using a CRISPR-based method and transferred the plasmid to a donor Escherichia coli strain.
Sweroside is a natural monoterpene derived from . Recently, studies have shown that sweroside exhibits a variety of biological activities, such as anti-inflammatory, antioxidant, and hypoglycemic effects. However, its role and mechanisms in high glucose (HG)-induced renal injury remain unclear.
View Article and Find Full Text PDFScrophulariae Radix (SR) has been widely used in Chinese herbal compound prescriptions, health care products and functional foods. The present study aimed to investigate the immunomodulatory activity of polysaccharides from SR (SRPs) in macrophages and explore the potential mechanisms. The results showed that four SRPs fractions (SRPs40, SRPs60, SRPs80 and SRPs100) had similar absorption peaks and monosaccharide compositions, but the intensities of absorption peaks and monosaccharide contents were distinguished.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
January 2024
Corn is an important food crop in the world. With economic development and population growth, the nutritional quality of corn is of great significance to high-quality breeding, scientific cultivation and fine management. Aiming at the problems of cumbersome steps, time-consuming and laborious, and low accuracy in the current research on corn quality detection.
View Article and Find Full Text PDFPML nuclear body (NB) malfunction often leads to acute leukemia outbreaks and other severe diseases. PML NB rescue is the molecular basis of arsenic success in acute promyelocytic leukemia (APL) treatment. However, it is unclear how PML NBs are assembled.
View Article and Find Full Text PDFSustainable nitrogen cycle is an essential biogeochemical process that ensures ecosystem safety and byproduct greenhouse gas nitrous oxide reduction. Antimicrobials are always co-occurring with anthropogenic reactive nitrogen sources. However, their impacts on the ecological safety of microbial nitrogen cycle remain poorly understood.
View Article and Find Full Text PDFThe establishment of symbiotic relationship between arbuscular mycorrhizal fungi (AMF) and roots is a mutually beneficial process and plays an important role in plant succession in ecosystems. However, there is less understanding of information about the AMF community in roots under vegetation succession on a large regional scale, especially the spatial variation in the AMF community and its potential ecological functions. Here, we elucidated the spatial variations in root AMF community structure and root colonization along a distribution pattern of four zonal Stipa species in arid and semiarid grassland systems and explored key factors regulating AMF structure and mycorrhizal symbiotic interactions.
View Article and Find Full Text PDFPeritoneal recurrence is the most frequent and lethal recurrence pattern in gastric cancer (GC) with serosal invasion after radical surgery. However, current evaluation methods are not adequate for predicting peritoneal recurrence in GC with serosal invasion. Emerging evidence shows that pathomics analyses could be advantageous for risk stratification and outcome prediction.
View Article and Find Full Text PDFAs a leaf homologous organ, soybean pods are an essential factor in determining yield and quality of the grain. In this study, a recognition method of soybean pods and estimation of pods weight per plant were proposed based on improved YOLOv5 model. First, the YOLOv5 model was improved by using the coordinate attention (CA) module and the regression loss function of boundary box to detect and accurately count the pod targets on the living plants.
View Article and Find Full Text PDFAppl Environ Microbiol
January 2023
Antibiotic resistance mediated by bacterial enzyme inactivation plays a crucial role in the degradation of antibiotics in the environment. Chloramphenicol (CAP) resistance by enzymatic inactivation comprises nitro reduction, amide bond hydrolysis, and acetylation modification. However, the molecular mechanism of enzymatic oxidation of CAP remains unknown.
View Article and Find Full Text PDFRegional PM transport is a crucial factor affecting air quality, and the meteorological mechanism in the atmospheric boundary layer (ABL) has not been fully understood over the receptor region in the regional transport of air pollutants. Based on the intensive vertical measurements of air pollutants and meteorology in the ABL during a transport-induced heavy air pollution event in Xiangyang, an urban site over a receptor region in central China, we investigated the meteorological mechanism in vertical PM changes in the ABL for heavy air pollution over the receptor region. Driven by northerly winds, regional PM transport was built from upstream northern China to downstream central China, where the observed ABL structures were unstable throughout the air pollution event.
View Article and Find Full Text PDFArbuscular mycorrhizal fungi (AMF) have been reported to have a wide distribution in terrestrial ecosystems and to play a vital role in ecosystem functioning and symbiosis with Stipa grasses. However, exactly how AMF communities in the rhizosphere change and are distributed along different Stipa population with substituted distribution and their relationships remain unclear. Here, the changes and distribution of the rhizosphere AMF communities and their associations between hosts and the dynamic differences in the glomalin-related soil protein (GRSP) in the rhizosphere soil of seven Stipa species with spatial substitution distribution characteristics in arid and semiarid grasslands were investigated.
View Article and Find Full Text PDFBackground: The modular inner branched stent-graft (MIBSG) (WeFlow-Arch™) is an emerging device for challenging aortic arch pathologies. Hemodynamic numerical simulation is conducive to predicting long-term outcomes as well as optimizing the stent-graft design.
Objective: This study aims to analyze the hemodynamic characteristics of the MIBSG devices based on numerical simulation analyses.