Comparative lipidomics analysis reveals changes in lipid profile of Arabica coffee at different maturity.

This study aimed to investigate the effects of maturity on the changes in major lipid metabolites of coffee and their associated pathways. UPLC-ESI-MS/MS was used to compare the lipidomic profiles of coffee beans at five different maturity stages. A total of 516 lipid metabolites across 26 subclasses were identified, with 111 showing significant differences.

An Identification Method for Road Hypnosis Based on the Fusion of Human Life Parameters.

A driver in road hypnosis has two different types of characteristics. One is the external characteristics, which are distinct and can be directly observed. The other is internal characteristics, which are indistinctive and cannot be directly observed.

Metabolic Engineering of for Self-Induced Production of l-Isoleucine.

As one of the three important branched-chain amino acids, l-isoleucine has a wide range of applications in the fields of medicine, food, and feed. Currently, the production of l-isoleucine is well-studied by , while the autonomous and efficient production of l-isoleucine in has not been reported. Here, we developed a production strategy that combined metabolic engineering with bacterial quorum sensing to achieve the efficient production of l-isoleucine.

Safety and efficacy of curcumin in the treatment of ulcerative colitis: An updated systematic review and meta-analysis of randomized controlled trials.

Objective: This study aims to systematically evaluate the safety and efficacy of curcumin as an adjunctive treatment for patients with ulcerative colitis (UC) and to assess the methodological quality of the published studies.

Methods: A comprehensive search was conducted in PubMed, Embase, and CENTRAL databases for randomized controlled trials published up to August 18, 2023. Two independent reviewers screened studies based on predefined criteria.

Nondigestible stachyose binds membranous HSP90β on small intestinal epithelium to regulate the exosomal miRNAs: A new function and mechanism.

Oligosaccharides are conventionally recognized as "passersby" in the small intestine. However, our research has reframed this understanding by uncovering a new function of oligosaccharide stachyose, which binds hydrophobic residues of membranous HSP90β on small intestinal epithelial cells, thus reprograming the exosomal miRNA profile. CRISPR-Cas9-mediated HSP90β knockout abolished the accumulation of stachyose on cell membrane and its regulatory effects on these miRNAs.

CMOS-Compatible High-Performance Silicon Nanowire Array Natural Light Electronic Detection System.

In this article, we propose a novel natural light detector based on high-performance silicon nanowire (SiNW) arrays. We achieved a highly controllable and low-cost fabrication of SiNW natural light detectors by using only a conventional micromachined CMOS process. The high activity of SiNWs leads to the poor long-term stability of the SiNW device, and for this reason, we have designed a fully wrapped structure for SiNWs.

A novel dialdehyde cellulose-based colorimetric and turn-on fluorescent probe for HS detection and its application in red wine.

Hydrogen sulfide (HS) is considered one of the most important gaseous transmitters in the metabolic system, and the abnormal concentration of HS is associated with a variety of diseases. Up to now, it is still a challenge to develop a portable assay for HS even though the research about the detection of HS is booming. Herein, a novel bifunctional dialdehyde-cellulose fluorescent probe DAC-DPD was prepared with high selectivity and sensitivity to HS with colorimetric and fluorescent "turn-on" characteristics, and the limit of detection (LOD) of DAC-DPD for HS was 0.

Design of a Membrane-Anchored DNAzyme-Based Molecular Machine for Enhanced Cancer Therapy by Customized Cascade Regulation.

Synthetic DNAzyme-based structures enable dynamic cell regulation. However, engineering an effective and targeted DNAzyme-based structure to perform customizable multistep regulation remains largely unexplored. Herein, we designed a membrane-anchored DNAzyme-based molecular machine to implement dynamic inter- and intracellular cascade regulation, which realizes efficient T-cell/cancer cell interactions and subsequent receptor mediated cancer cell uptake.

Huopuxialing Decoction: A Promising Candidate for Precancerous Lesions of Gastric Cancer Treatment Based on Bioinformatics and Experimental Verification.

Background: The Precancerous Lesion of Gastric Cancer (PLGC) is an early stage in the development of gastric cancer. The clinical application of HPXLD has been found to be effective in treating PLGC, but the mechanism of how HPXLD acts on PLGC is still unclear.

Objective: The objectives of this study were to reveal the molecular mechanism of how HPXLD can be used to treat PLGC and investigate this mechanism through bioinformatics and experimental validation.

Deteriorating wintertime habitat conditions for waterfowls in Caizi Lake, China: Drivers and adaptive measures.

China has made enormous strides to achieve high-quality development and biodiversity conservation, and the establishment of nature-protected areas is one of the essential initiatives. Caizi Lake involves a natural reserve and two national wetland parks, accommodating winter migratory waterfowl over the middle and lower Yangtze River basin in China. However, the water transfer from the Yangtze River to the Huai River (YR-HR water transfer) has modified the winter hydrological conditions of Caizi Lake, negatively affecting wintertime waterfowl habitats.

Green electrospun Fe-based MOFs incorporated polyvinyl alcohol/carboxymethyl chitosan nanofibrous membranes for enhanced adsorption of tetracycline hydrochloride.

Tetracycline hydrochloride (TCH) removal from wastewater has drawn much attention recently, although it still remains challenging. Herein, Fe-based metal-organic frameworks (MOFs) incorporated nanofibrous membranes were prepared by green electrospinning and applied as adsorbents to remove TCH. The presence of MOFs noticeably improved specific surface area of the nanofibrous membranes, and adsorption capability increased with the amount of MOFs within membranes.

Spatial Mapping of Bioactive Metabolites in the Roots of Three Species by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging.

is a kind of medicinal plant that has made a great contribution to human health because of the presence of bioactive metabolites: saikosaponins and flavonoids. Despite their importance, it has been a challenge to visually characterize the spatial distribution of these metabolites in situ within the plant tissue, which is essential for assessing the quality of . The development of a new technology to identify and evaluate the quality of medicinal plants is therefore necessary.

Advanced glycation end products (AGEs) impair the intestinal epithelial barrier via STAT3 activation mediated by macrophages.

Advanced glycation end products (AGEs) are a spectrum of complex compounds widely found in processed foods and frequently consumed by humans. AGEs are implicated in impairing the intestinal barrier, but the underlying mechanisms remain unclear. This study investigated the effects of three types of AGEs on gene expression of tight junctions (TJs) in colorectal epithelial HT-29 cells, and observed minimal alterations in TJs expression.

Characterization of a secondary palmitoleoyltransferase of lipid A in Vibrio parahaemolyticus.

The detection of pathogenicity and immunogenicity in Vibrio parahaemolyticus poses a significant challenge due to its threat to human health and food safety, which is strongly correlated with lipid A. Lipid A, a critical component found in most Gram-negative bacteria, functions as a hydrophobic anchor for lipopolysaccharide. V.

Tailored Borneol-Modified Lipid Nanoparticles Nasal Spray for Enhanced Nose-to-Brain Delivery to Central Nervous System Diseases.

The nanodrug delivery system-based nasal spray (NDDS-NS) can bypass the blood-brain barrier and deliver drugs directly to the brain, offering unparalleled advantages in the treatment of central nervous system (CNS) diseases. However, the current design of NNDS-NS is excessively focused on mucosal absorption while neglecting the impact of nasal deposition on nose-to-brain drug delivery, resulting in an unsatisfactory nose-to-brain delivery efficiency. In this study, the effect of the dispersion medium viscosity on nasal drug deposition and nose-to-brain delivery in NDDS-NS was elucidated.

Optimizing commercial Arabica coffee quality by integrating flavor precursors with anaerobic germination strategy.

This study attempted to improve commercial Arabica coffee quality by integrating flavor precursors with anaerobic germination. Using raw coffee beans as materials, anaerobic germination was conducted with 5 g/100 g of flavor precursors (sucrose, glucose, fructose). The chemical composition and sensory quality of roasted coffee beans were analyzed.

Breastfeeding may reduce the effects of maternal smoking on lung cancer mortality in adult offspring: a prospective cohort study.

Background: Although previous research has indicated a correlation between smoking and the mortality rate in patients with lung cancer, the impact of early life factors on this relationship remains unclear and requires further investigation. This study aimed to investigate the hypothesis that breastfeeding reduces the risk of lung cancer-related death.

Methods: The authors conducted a prospective cohort study involving 501 859 participants recruited from the United Kingdom Biobank to explore the potential association between breastfeeding and the risk of lung cancer mortality using a Cox proportional hazards model.

Perfluorooctanoic acid (PFOA) and its alternative perfluorobutanoic acid (PFBA) alter hepatic bile acid profiles via different pathways.

The disruption of per- and polyfluoroalkyl substances (PFASs) on bile acid (BA) homeostasis has raised public concerns, making the evaluation of their effects and underlying mechanisms a high priority. Although the use of perfluorooctanoic acid (PFOA) has been restricted, it remains a widespread legacy PFAS in the environment. Concurrently, the use of its prevalent short-chain alternative, perfluorobutanoic acid (PFBA), is increasing, yet the toxicity assessment of PFBA remains inadequate.

Engineering mesoporous polydopamine-based potentiate STING pathway activation for advanced anti-biofilm therapy.

The biofilm-induced "relatively immune-compromised zone" creates an immunosuppressive microenvironment that is a significant contributor to refractory infections in orthopedic endophytes. Consequently, the manipulation of immune cells to co-inhibit or co-activate signaling represents a crucial strategy for the management of biofilm. This study reports the incorporation of Mn into mesoporous dopamine nanoparticles (Mnp) containing the stimulator of interferon genes (STING) pathway activator cGAMP (Mncp), and outer wrapping by M1-like macrophage cell membrane (m-Mncp).

The Impact of School Reopening on Chinese Adolescents' Mental Health During COVID-19: Considering the Role of Academic Stress and Academic Orientation.

Purpose: Existing studies found that school closure during the COVID-19 pandemic negatively influenced adolescents' mental health. Yet, it remains unclear how adolescent mental health changed during the transition of school reopening as well as the academic-related risk and protective factors.

Methods: Immediately before (April 2020) and three months (July 2020) after school reopening, 879 adolescents in Shanghai, China (mean age = 13.