MOF scaffold for anchoring platinum-nickel nanoparticles with enhanced oxidase-like activity to improve lateral flow immunoassay diagnosis.

Noble metal nanoparticles have attracted tremendous attention as the promising signal reporters for catalytic-colorimetric lateral flow immunoassay (LFIA). However, it remains great challenges for improving their stability and catalytic activity. Herein, first, a kind of porphyrinic based metal-organic framework (MOF) was used as a carrier for loading platinum (Pt) nanoparticles to avoid its aggregation.

Aptamer-Proximity Ligation Coupled with Rolling Circle Amplification Strategy for an Ultrasensitive Analysis of Tumor-Derived Extracellular Vesicles PD-L1.

Tumor-derived extracellular vesicles (T-EVs) PD-L1 are an important biomarker for predicting immunotherapy response and can help us understand the mechanism of resistance to immunotherapy. However, this is due to the interference from a large proportion of nontumor-derived EVs. It is still challenging to accurately analyze T-EVs PD-L1 in complex human fluids.

Dual-mode sensing platform for Bisphenol A detection via bifunctional CsPbBr@Cu-MOF assisted fluorescence and colorimetric analysis.

Background: Bisphenol A (BPA) has been identified as an endocrine disruptor with numerous detrimental effects on human health. There is an urgent need to develop fluorescence/colorimetric dual-mode sensing approaches with expanded detection linear range, increased accuracy, and enhanced application flexibility for BPA detection. The utilization of fluorescence and colorimetric signals in point-of-care applications and real-time sensitive sensing further highlights the significance of developing novel and efficient fluorescence/colorimetric dual-mode sensing platform with high-efficiency probes.

Knockdown of the long noncoding RNA VSIG2-1:1 promotes the angiogenic ability of human pulmonary microvascular endothelial cells by activating the VEGF/PI3K/AKT pathway.

Background: Abnormal pulmonary vascular development poses significant clinical challenges for infants with bronchopulmonary dysplasia (BPD). Although numerous factors have been suggested to control the development of pulmonary blood vessels, the mechanisms underlying the role of long noncoding RNAs (lncRNAs) in this process remain unclear.

Methods: A lncRNA array was used to measure the differential expression of lncRNAs in premature infants with and without BPD.

Alterations in subgingival microbiome and advanced glycation end-products levels in periodontitis with and without type 1 diabetes mellitus: a cross-sectional study.

Background: Existing studies predominantly focused on the relationship between periodontitis and type 2 diabetes mellitus (T2DM), with limited data on the association between periodontitis and type 1 diabetes mellitus (T1DM). This study aimed to examine the impact of T1DM and periodontitis on the subgingival microbiome and levels of advanced glycation end-products (AGEs).

Methods: Samples were collected from four groups: T1DM, periodontitis (P), T1DM with periodontitis (DP), and periodontally and systemically healthy controls (Control).

Novel self-powered sandwich type Aptamer sensor for estrogen detection assisted with peroxidase mimic Cu-MOF.

A novel self-powered and flexible enzymatic biofuel cell (EBFC)-based aptasensor was developed for the sensitive and selective detection of 17 β-estradiol (E2). A flexible polyvinyl alcohol (PVA)-tannic acid‑carbon nanotube/reduced graphene oxide (PTCR) substrate was modified with gold nanoparticles (AuNPs) and thiolated aptamer 1 (Apt1) to yield Apt1@AuNPs/PTCR. A copper-based metal-organic framework (Cu-MOF) with peroxidase mimicking activity was employed to anchor glucose oxidase (GOD) and Apt2, forming the Cu-MOF@GOD/Apt2 tag.

A generalizable sensing platform based on molecularly imprinted polymer-aptamer double recognition and nanoenzyme assisted photoelectrochemical-colorimetric dual-mode detection.

Developing highly sensitive and selective methods that incorporate specific recognition elements is crucial for detecting small molecules because of the limited availability of small molecule antibodies and the challenges in obtaining sensitive signals. In this study, a generalizable photoelectrochemical-colorimetric dual-mode sensing platform was constructed based on the synergistic effects of a molecularly imprinted polymer (MIP)-aptamer sandwich structure and nanoenzymes. The MIP functionalized peroxidase-like FeO (FeO@MIPs) and alkaline phosphatase mimic Zr-MOF labeled aptamer (Zr-mof@Apt) were used as the recognition elements.

A dual-mode "signal-on" split-type aptasensor for bisphenol A target-induced hybridization chain reaction amplification.

Herein, a dual-mode detection system was constructed for efficient and accurate detection of bisphenol A (BPA) with the assistance of the BPA-induced hybridization chain reaction (HCR). The captured DNA (cDNA) was first modified on the surface of magnetic spheres modified with gold nanoparticles and polydopamine and then hybridized with the BPA aptamer to form double-stranded DNA (dsDNA). In the presence of the BPA target, the BPA aptamer was released from the surface of the magnetic sphere.

Wearable electrochemical sensor based on bimetallic MOF coated CNT/PDMS film electrode via a dual-stamping method for real-time sweat glucose analysis.

Non-invasive wearable sweat glucose sensors are expected to be highly desirable for personalized diabetes management. Therefore, developing facile, convenient, and scalable manufacturing method of such wearable sensors is urgently needed. Herein, we report a simple and low-cost stamping-vacuum filtration dry transfer (SVFDT) method for construction of a wearable sweat glucose electrochemical sensor.

Functional Zonation Strategy of Heterodimer Nanozyme for Multiple Chemiluminescence Imaging Immunoassay.

Although nanozymes with intrinsic enzyme-like characteristics have aroused great interest in the biosensing field, the challenge is to keep high enzyme-like activity of the nanozyme after the modification of biomolecules onto nanozymes. Herein, a functional zonation strategy of a heterodimer nanozyme was proposed to tackle the challenge and further construct a multiple chemiluminescence (CL) imaging immunoassay. Here FeO-Au as a heterodimer nanozyme model was divided into two zones, in which FeO nanoparticles (NPs) were regarded as a nanozyme zone and AuNPs were defined as an antibody immobilization zone.

A bimetallic metal-organic framework with high enzyme-mimicking activity for an integrated electrochemical immunoassay of carcinoembryonic antigen.

Metal-organic frameworks (MOFs) show excellent catalytic activity and have been widely applied in diagnosis of diseases and tumors. However, current assay methods usually involve cumbersome configurations and complicated procedures, which inhibit their practical applications. Hence, a Cu-Ni MOF/carbon printed electrode (CPE)-based integrated electrochemical immunosensor was constructed for highly sensitive and efficient determination of carcinoembryonic antigen (CEA).

Amphiphilic Polymer Capped Perovskite Compositing with Nano Zr-MOF for Nanozyme-Involved Biomimetic Cascade Catalysis.

CsPbX perovskite nanocrystal (NC) is considered as an excellent optical material and is widely applied in optoelectronics. However, its poor water stability impedes its study in enzyme-like activity, and further inhibits its application in biomimetic cascade catalysis. Herein, for the first time, the oxidase-like and ascorbate oxidase-like activities of an amphiphilic polymer capped CsPbX are demonstrated, and its catalytic mechanism is further explored.

NLRC4 gain-of-function variant is identified in a patient with systemic lupus erythematosus.

NLRC4 gain-of-function variants are known to cause various autoinflammatory phenotypes, including familial cold autoinflammatory syndrome (FCAS4) and NLRC4 macrophage activation syndrome (NLRC4-MAS). However, to date, no study has linked NLRC4 gain-of-function variants to systemic lupus erythematosus (SLE). In this study, we identified a novel NLRC4 W655S variant in an SLE patient and her son, who had neonatal lupus complicated with macrophage activation syndrome.

Molecularly imprinted ultrasensitive cholesterol photoelectrochemical sensor based on perfluorinated organics functionalization and hollow carbon spheres anchored organic-inorganic perovskite.

In spite of organic-inorganic perovskite emerging as a novel efficient light-harvesting material owing to their superior optical properties, excitonic properties, and electrical conductivity, the related applications are severely limited for their poor stability and selectivity. Herein, we introduced hollow carbon spheres (HCSs) and 2-(perfluorohexyl) ethyl methacrylate (PFEM) based molecularly imprinted polymers (MIPs) to dual-functionalize CHNHPbI. HCSs can provide perovskite load conditions, passivate perovskite defects, increase carrier transport and effectively improve its hydrophobicity.

A wearable sweat electrochemical aptasensor based on the Ni-Co MOF nanosheet-decorated CNTs/PU film for monitoring of stress biomarker.

Monitoring cortisol, a hormone released by the adrenal cortex in response to stress, is essential to evaluate the endocrine response to stress stimuli. While the current cortisol sensing methods require large laboratory settings, complex assay, and professional personnel. Herein, a novel flexible and wearable electrochemical aptasensor based on a Ni-Co metal-organic frameworks (MOF) nanosheet-decorated carbon nanotubes (CNTs)/polyurethane (PU) film is developed for rapid and reliable detection of cortisol in sweat.

Encoded Fusion-Mediated MicroRNA Signature Profiling of Tumor-Derived Extracellular Vesicles for Pancreatic Cancer Diagnosis.

MicroRNAs (miRNAs) in tumor-derived extracellular vesicles (tEVs) are important cancer biomarkers for cancer screening and early diagnosis. Multiplex detection of miRNAs in tEVs facilitates accurate diagnosis but remains a challenge. Herein, we propose an encoded fusion strategy to profile the miRNA signature in tEVs for pancreatic cancer diagnosis.

Roles of Pyroptosis-Related Genes in the Diagnosis and Subtype Classification of Periodontitis.

Pyroptosis is widely involved in many diseases, including periodontitis. Nonetheless, the functions of pyroptosis-related genes (PRGs) in periodontitis are still not fully elucidated. Therefore, we aimed to investigate the role of PRGs in periodontitis.

An integrated and flexible PDMS/Au film-based electrochemical immunosensor via Fe-Co MOF as a signal amplifier for alpha fetoprotein detection.

Ultrasensitive determination of tumor marker (TM) is of great significance in cancer prevention and diagnosis. Traditional TM detection methods involve large instrumentation and professional manipulation, which complicate the assay procedures and increase the cost of investment. To resolve these problems, an integrated electrochemical immunosensor based on the flexible polydimethylsiloxane/gold (PDMS/Au) film with Fe-Co metal-organic framework (Fe-Co MOF) as a signal amplifier was fabricated for ultrasensitive determination of alpha fetoprotein (AFP).

β-BiO Nanosheets Functionalized with Bisphenol A Synthetic Receptors: A Novel Material for Sensitive Photoelectrochemical Platform Construction.

In this study, β-BiO nanosheets functionalized with bisphenol A (BPA) synthetic receptors were developed by a simple molecular imprinting technology and applied as the photoelectric active material for the construction of a BPA photoelectrochemical (PEC) sensor. BPA was anchored on the surface of β-BiO nanosheets via the self-polymerization of dopamine monomer in the presence of a BPA template. After the elution of BPA, the BPA molecular imprinted polymer (BPA synthetic receptors)-functionalized β-BiO nanosheets (MIP/β-BiO) were obtained.

Ultra-stable CsPbBr@PbBrOH nanorods for fluorescence labeling application based on methylimidazole-assisted synthesis.

The extension application of perovskites in aqueous media such as bioassays requires the development of a water-stable perovskite with a simple preparation process and low cost. However, the degradation of perovskites in aqueous solution is still a thorny problem. Here, we develop a methylimidazole-assisted two-step synthesis protocol to prepare CsPbBr@PbBrOH nanorods with superior water stability and remarkable optical properties at room temperature.

PP-DDP: a privacy-preserving outsourcing framework for solving the double digest problem.

Background: As one of the fundamental problems in bioinformatics, the double digest problem (DDP) focuses on reordering genetic fragments in a proper sequence. Although many algorithms for dealing with the DDP problem were proposed during the past decades, it is believed that solving DDP is still very time-consuming work due to the strongly NP-completeness of DDP. However, none of these algorithms consider the privacy issue of the DDP data that contains critical business interests and is collected with days or even months of gel-electrophoresis experiments.