HCAR2 Modulates the Crosstalk between Mammary Epithelial Cells and Macrophages to Mitigate Staphylococcus aureus Infection in the Mouse Mammary Gland.

Staphylococcus aureus (S. aureus) is a major zoonotic pathogen, with mammary gland infections contributing to mastitis, a condition that poses significant health risks to lactating women and adversely affects the dairy industry. Therefore, understanding the immune mechanisms underlying mammary infections caused by S.

Highly Selective AIEgen-Based "Turn On" Fluorescent Imaging for Inflammation Detection.

Hypochlorous acid (HClO) is released by immune cells in the immune system, and it helps the body fight off infections and inflammation by killing bacteria, viruses, and other pathogens. However, tissue damage or apoptosis may also be induced by excess HClO. On this basis, we designed the probe TPE-NS by choosing tetraphenylethylene (TPE) as the luminescent unit and dimethylthiocarbamoyl chloride as the recognition site.

Dnmt3a-mediated DNA Methylation Regulates P. gingivalis-suppressed Cementoblast Mineralization Partially Via Mitochondria-dependent Apoptosis Pathway.

Background: DNA methyltransferase 3A (Dnmt3a) is an enzyme that catalyzes the de novo methylation of DNA, and plays essential roles in a wide range of physiological and pathological processes. However, it remains unclear whether Porphyromonas gingivalis affects cementoblasts, the cells responsible for cementum formation, through Dnmt3a.

Methods: The samples were collected from models of mouse periapical lesions and mice of different ages, and the expression of Dnmt3a was detected through immunofluorescence.

Identification and Functional Analysis of the Gene Conferring Resistance to Late Blight () in Tomato.

Late blight is a destructive disease affecting tomato production. The identification and characterization of resistance (R) genes are critical for the breeding of late blight-resistant cultivars. The incompletely dominant gene confers resistance against the race T of in tomatoes.

Pathogenic mechanisms and potential applications of extracellular vesicles from periodontal pathogens in periodontitis.

Periodontitis is a multifactorial disease characterized by chronic destruction of the periodontal supporting tissues and is closely associated with the dysbiosis of the plaque biofilm. It is the leading cause of tooth loss in adults. Bacterial extracellular vesicles (BEVs) are released from bacteria, which range in size from 20 to 400 nm.

Melanin-concentrating hormone attenuates the hedonic feeding induced by orexin-A in the ventral tegmental area of high-fat diet male mice.

Objective: The ventral tegmental area (VTA), a pivotal hub in the brain's reward circuitry, receives inputs from the lateral hypothalamic area (LHA). However, it remains unclear whether melanin-concentrating hormone (MCH) and orexin-A (OX-A) neurons in the LHA exert individual or cooperative influence on palatable food consumption in the VTA. This study aims to investigate the modulatory role of MCH and OX-A in hedonic feeding within the VTA of high-fat diet (HFD) mice.

METTL3-mediated m6A modifications of NLRP3 accelerate alveolar bone resorption through enhancing macrophage pyroptosis.

Periodontitis (PD) is twice as prevalent in diabetics compared to nondiabetics, and diabetes-associated PD is characterized by increased inflammation and aggravated tissue damage. Pyroptosis has recently been implicated in diabetes-associated PD; however, the underlying mechanisms remain largely unknown, resulting in a lack of effective treatments. In this study, we investigated the role of methyltransferase-like 3 (METTL3) in macrophage pyroptosis and found that it inhibits the osteogenic differentiation of osteoblasts via pyroptotic macrophages in a diabetes-associated periodontitis mouse model.

[Research progress on indirect action and mechanism of traditional Chinese medicine based on gut-gut microbiota-target organ talks].

Traditional Chinese medicine(TCM) has been treating diseases for thousands of years and still holds an irreplaceable position in modern disease treatment. The composition of TCM is complex, and its mode of action features multi-pathway and multi-target characteristics, making its pharmacological actions and mechanisms complicated. The principles and mechanisms of TCM have always been a hot topic among scholars from various fields.

Effects of electromagnetic field emitted by a 90 kHz WPT system on the cognitive functions and neuronal excitation of mice.

The advantages of Magnetic Coupling Resonant Wireless Power Transfer (MCR-WPT) technology include long transmission distance, high efficiency, and high power. Therefore, it shows great potential in the field of smart home. This study aims to explore the specific impacts on the cognitive functions and neuronal excitation of mice exposed to the electromagnetic fields (EMF) emitted by the MCR-WPT platform, thereby providing biological solid experimental evidence for developing Wireless Power Transfer (WPT) technology.

Astragalus polysaccharide alleviates mastitis disrupted by Staphylococcus aureus infection by regulating gut microbiota and SCFAs metabolism.

Polysaccharides, key bioactive compounds derived from Chinese herbs, are increasingly recognized for their therapeutic potential in modulating gut microbiota to treat various diseases. However, their efficacy in alleviating mammary inflammation and oxidative stress and protecting the blood-milk barrier (BMB) compromised by Staphylococcus aureus (S. au) infection remains uncertain.

Development and validation of a prognostic and drug sensitivity model for gastric cancer utilizing telomere-related genes.

Background: Gastric cancer (GC) poses a major global health challenge because of its unfavorable prognosis. Elevated telomerase activity has been linked to the rapid growth and invasiveness of GC tumors. Investigating the expression profiles of telomerase could improve our understanding of the mechanisms underlying telomere-related GC advancement and its applicability as potential targets for diverse therapeutic strategies for GC.

Dissection of major QTLs and candidate genes for seedling stage salt/drought tolerance in tomato.

Background: As two of the most impactful abiotic stresses, salt and drought strongly affect tomato growth and development, especially at the seedling stage. However, dissection of the genetic basis underlying salt/drought tolerance at seedling stage in tomato remains limited in scope.

Results: Here, we reported an analysis of major quantitative trait locus (QTL) and potential causal genetic variations in seedling stage salt/drought tolerance in recombinant inbred lines (n = 201) of S.

Longitudinal Trajectories of Psychological Distress and Its Influence Factors in Young and Middle-Aged Patients With Colorectal Cancer.

Aims: To identify the longitudinal trajectories of psychological distress and to explore the predictive factors of different trajectories among young and middle-aged colorectal cancer patients from diagnosis to 6 months postoperation.

Design: A longitudinal study was conducted, guided by the STROBE checklist.

Methods: A total of 214 patients were recruited from July 2021 to May 2022 at a cancer hospital in Guangzhou, China.

KLF4's role in regulating nitric oxide production and promoting microvascular formation following ischemic stroke.

This study examines KLF4's role in endothelial cells (ECs), emphasizing its effects on nitric oxide (NO) production, microvascular formation, and oxidative stress regulation following ischemic stroke. Through high-throughput sequencing, we identified eight cell subpopulations in carotid artery tissues post-stroke, with KLF4 notably elevated in ECs. KLF4 overexpression in ECs promoted NO synthesis, enhanced endothelial tube formation, mitigated oxidative stress, and improved smooth muscle cells (SMCs) function, collectively boosting blood flow in ischemic regions.

A nomogram model for predicting frailty in middle-aged and elderly patients with colorectal cancer:A cross-sectional study.

Purpose: Frailty is a syndrome caused by multiple factors and can lead to serious consequences for middle-aged and elderly patients with colorectal cancer. However, few studies have comprehensively explored predictors of frailty and built predictive models. Therefore, our aim is to develop and evaluate a predictive model for frailty in middle-aged and elderly patients with colorectal cancer.

Autophagy mediates the impact of Porphyromonas gingivalis on short-chain fatty acids metabolism in periodontitis-induced gut dysbiosis.

Porphyromonas gingivalis (P. gingivalis), the main pathogen responsible for periodontitis, is linked to systemic disorders via the oral-gut axis. Short-chain fatty acids (SCFAs) are vital for gut health, but their role in P.

Synthesis of Dimethyl Hexane-1,6-dicarbamate with Methyl Carbamate as Carbonyl Source over MCM-41 Catalyst.

Dimethyl hexane-1,6-dicarbamate (HDC), the vital intermediate for nonphosgene production of hexamethylene-diisocyanate (HDI), was effectively synthesized via carbonylation of 1,6-hexanediamine (HDA) using methyl carbamate (MC) as a carbonyl source over a silanol-rich MCM-41 catalyst. The effects of reaction conditions, including the reaction temperature, molar ratio of raw materials, methanol dosage, catalyst dosage, and reaction time, on the HDC yield were evaluated. Under the reaction conditions with a reaction temperature of 190 °C, a molar ratio of HDA, MC, and methanol of 1:6:50, a catalyst dosage of 10 wt %, and a reaction time of 3 h, the yield of HDC can reach as high as 92.

Distributed Algorithms for Linear Equations Over General Directed Networks.

This article deals with linear equations of the form Ax = b . By reformulating the original problem as an unconstrained optimization problem, we first provide a gradient-based distributed continuous-time algorithm over weight-balanced directed graphs, in which each agent only knows partial rows of the augmented matrix (A b) . The algorithm is also applicable to time-varying networks.

Antibacterial Activity and Multi-Targeted Mechanism of Action of Suberanilic Acid Isolated from DCL44: An Endophytic Fungi from .

Methicillin-resistant (MRSA) is a highly threatening foodborne pathogen capable of causing severe organ and life-threatening diseases. Over the past years, various commercial antibiotics have been used to treat MRSA infections. However, these commercial antibiotics have not yielded efficient results and also cause other side effects; therefore, there is a need for the development of effective alternatives to replace these commercial antibiotics.

Associations of the planetary health diet index (PHDI) with asthma: the mediating role of body mass index.

Background: Given the global shifts in environmental conditions and dietary habits, understanding the potential impact of dietary factors and body mass index (BMI) on respiratory diseases, including asthma, is paramount. Investigating these relationships can contribute to the formulation of more effective prevention strategies. The Planetary Health Diet Index (PHDI), a dietary scoring system that balances human health with environmental sustainability, underscores the importance of increasing the consumption of plant-based foods while reducing the intake of red meat, sugar, and highly processed foods.

Deep learning based approach: automated gingival inflammation grading model using gingival removal strategy.

Gingival inflammation grade serves as a well-established index in periodontitis. The aim of this study was to develop a deep learning network utilizing a novel feature extraction method for the automatic assessment of gingival inflammation. T-distributed Stochastic Neighbor Embedding (t-SNE) was utilized for dimensionality reduction.