[Study for association between high risk human papillomavirus and cervical lesions in the samples from opportunistic screening].

Objective: To investigate the association between HPV genotypes and cervical lesion in Hybrid Capture 2 (HC2) HPV test positive samples.

Methods: 602 cervical samples randomly obtained detected as HPV positive by the HC2 high-risk probe cocktail were determined by polymerase chain reaction (PCR)-reverse dot blot (RDB), among them 344 participated Thinprep Cytology test.

Results: 569 (94.

[Study of human papillomavirus in small cell neuroendocrine carcinoma of the uterine cervix].

Objective: To observe the infection of the high risk human papillomavirus (HPV) in the specimen of the small cell neuroendocrine carcinoma of the uterine cervix (SCNEC).

Methods: We extracted the nucleic acids in the formalin-fixed and paraffin-embedded specimen from a 33-year-old patient diagnosed as small cell neuroendocrine carcinoma of uterine cervix and detected the HPV genotype with the nested PCR.

Results: We identified HPV18, a high-risk genotype, in the specimen.

[Human papillomavirus type 16 E5 sequence evolution analysis].

Objective: To investigate the distribution of HPV 16 variants in Han women patients without Cervical intraepithelial neoplasia in the diagnosis and treatment center for cervical disease, department of Obstetrics and Gynecology in China-Japan friendship hospital with HPV 16 E5 sequence phylogenetic analysis.

Methods: PCR amplification of HPV 16 E5 sequences and sequenced. The association between variations types and different cervical lesions was analyzed.

[Detection of human papillomavirus in esophageal carcinoma tissues from Baoding City of Hebei Province].

Objective: To investigate the relationship between the human papillomavirus (HPV) and esophageal carcinoma in Baoding City of Hebei Province.

Methods: We detected HPV DNA in 42 formalin-fixed and paraffin-embedded tissues from surgically resected esophageal carcinomas from Baoding City of Hebei Province, by PCR with the general primer set of GP5 + /6 + for HPV L1 gene and type-specific primer sets for HPV16 and 18 as well.

Results: 37 from 42 esophageal carcinoma samples were HPV positive and the rate was 88.

[Meta analysis on etiological relationship between human papillomavirus and esophageal carcinoma].

Objective: To study the relationship between human papillomavirus (HPV) and esophageal cancer development in China.

Methods: We searched and collected the published articles in Chinese related to HPV and esophageal cancer, and selected the articles with the PCR approach to detect HPV in the esophageal cancer specimens.

Results: We filtered our publication collection with standards as (1) PCR as the detection approach, (2) specimens as the paraffin-embedded sections, and (3) description of the primer in the experiments, and fifteen articles were enrolled for our meta-analysis.

[Human papillomavirus detection in clear cell carcinoma of the cervix].

Objective: To investigate the infection of the high risk human papillomavirus (HPV) in the specimen of the clear cell carcinoma of the cervix.

Methods: We extracted the nucleic acids in the formalin-fixed and paraffin-embedded specimens from a 37-year-old patient with clear cell carcinoma of the cervix and detected the HPV genotype with the nested PCR.

Results: We identified HPV18, a high-risk genotype, in the specimens.

[Detection of human papillomavirus in tissues of esophageal carcinomas by polymerase chain reaction].

Objective: To investigate the relationship between the human papillomavirus (HPV) and esophageal carcinomas.

Methods: We detected HPV DNA in 31 fresh tissue of esophageal carcinomas from Linzhou City, Henan Province, by PCR with the general primer set of GP5+/6+ for HPV L1 gene and type-specific primer sets for HPV16 and 18 as well.

Results: 29 from 31 esophageal carcinoma samples were HPV positive and the rate was 93.

A novel cDNA encodes a putative hRALY-like protein, hRALYL.

High throughput cDNA sequencing and 5'-rapid amplification of cDNA ends (5'RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.