Flavopiridol induces cell cycle arrest and apoptosis by interfering with CDK1 signaling pathway in human ovarian granulosa cells.

Several clinical trials have been conducted to evaluate the use of flavopiridol (FP) to treat a variety of cancers, and almost all cancer drugs were found to be associated with toxicity and side effects. It is not clear whether the use of FP will affect the female reproductive system. Granulosa cells, as the important cells that constitute the follicle, play a crucial role in determining the reproductive ability of females.

Comparative analyses of the transcriptome among three development stages of Zeugodacus tau larvae (Diptera: Tephritidae).

Studying differences in transcriptomes across various development stages of insects is necessary to uncover the physiological and molecular mechanism underlying development and metamorphosis. We here present the first transcriptome data generated under Illumina Hiseq platform concerning Zeugodacus tau (Walker) larvae from Nanchang, China. In total, 11,702 genes were identified from 9 transcriptome libraries of three development stages of Z.

Flavopiridol inhibits oocyte maturation, reduces oocyte quality and blocks cumulus cell function.

Flavopiridol (FP) is a plant-derived flavonoidis and used to treat cancers, fungal infections and inflammation-related diseases. However, it is not clear whether it has side effects on the female reproductive system. In this study, we aimed to investigate the toxic effects and potential underlying mechanisms of FP on oocyte maturation and cumulus cell expansion in mice.

Gut microbiota-bile acid-vitamin D axis plays an important role in determining oocyte quality and embryonic development.

Objective: To reveal whether gut microbiota and their metabolites are correlated with oocyte quality decline caused by circadian rhythm disruption, and to search possible approaches for improving oocyte quality.

Design: A mouse model exposed to continuous light was established. The oocyte quality, embryonic development, microbial metabolites and gut microbiota were analyzed.

4-vinylcyclohexene diepoxide induces apoptosis by excessive reactive oxygen species and DNA damage in human ovarian granulosa cells.

4-Vinylcyclohexene diepoxide (VCD) is a hazardous industrial material which is widely used in the production of fragrances, rubber tires, antioxidants, pesticides, flame retardants and plasticizers. Previous studies have shown that exposure to VCD damages the female reproductive system, but the effects and mechanisms of VCD exposure on human granulosa cells are not reported. In this study, we used a human granulosa cell line (SVOG) to explore the effects of VCD exposure and found that VCD exposure had toxic effects on SVOG cells in vitro.

USP7 reduction leads to developmental failure of mouse early embryos.

As a member of Ubiquitin-specific protease subfamily, ubiquitin specific protease 7 (USP7) has been reported to participate in a variety of cellular processes, including cell cycle, apoptosis, DNA damage response, and epigenetic modification. However, its function in preimplantation embryos is still obscure. To investigate the functions of USP7 during preimplantation embryo development, we used siRNA to degrade endogenous USP7 messenger RNA.

Effects of Photodynamic Therapy on Nav1.7 Expression in Spinal Dorsal Root Ganglion Neurons.

Objective: The aim of this study was to examine the effects of photodynamic therapy (PDT) on the expression of Nav1.7 in spinal dorsal root ganglion (DRG) neurons.

Methods: The primary DRG neurons from newborn SD rats were cultured.

Maternal exposure to 4-vinylcyclohexene diepoxide during pregnancy induces subfertility and birth defects of offspring in mice.

4-vinylcyclohexene diepoxide (VCD), widely used in industry, is a hazardous compound that can cause premature ovarian failure, but whether maternal VCD exposure affects the health and reproduction of offspring is unknown. Here we focused on the effects of VCD on fertility and physical health of F1 and F2 offspring in mice. The pregnant mice were injected intraperitoneally with different dosages of VCD once every day from 6.

Stereocontrolled Self-Assembly of Ln(III)-Pt(II) Heterometallic Cages with Temperature-Dependent Luminescence.

Structurally well-defined discrete d/f heterometallic complexes show diverse application potential in electrooptic and magnetic materials. However, precise control of the component and topology of such heterometallic compounds with fine-tuned photophysical properties is still challenging. Herein, we report the stereocontrolled syntheses of a series of Ln-Pt heterometallic cages through coordination-driven self-assembly of enantiopure alkynylplatinum-based metalloligands (L, L) with lanthanide ions (Ln = Eu, Yb, Nd, Lu).

Analysis of Chinese Medical Syndrome Features of Ischemic Stroke Based on Similarity of Symptoms Subgroup.

Objective: To derive the Chinese medicine (CM) syndrome classification and subgroup syndrome characteristics of ischemic stroke patients.

Methods: By extracting the CM clinical electronic medical records (EMRs) of 7,170 hospitalized patients with ischemic stroke from 2016 to 2018 at Weifang Hospital of Traditional Chinese Medicine, Shandong Province, China, a patient similarity network (PSN) was constructed based on the symptomatic phenotype of the patients. Thereafter the efficient community detection method BGLL was used to identify subgroups of patients.

Optimal indicator for changing the filter during the continuous renal replacement therapy in intensive care unit patients with acute kidney injury: A crossover randomized trial.

Background: The study aims to investigate an optimal indicator for changing the filter during the continuous renal replacement therapy (CRRT) in intensive care unit (ICU) patients with acute kidney injury (AKI).

Methods: Patients with AKI requiring CRRT in an ICU were randomly divided into two groups for crossover trial, i.e.

Guest-Driven Self-Assembly and Chiral Induction of Photofunctional Lanthanide Tetrahedral Cages.

Chiral luminescent lanthanide-organic cages have many potential applications in enantioselective recognition, sensing, and asymmetric catalysis. However, due to the paucity of structures and their limited cavities, host-guest chemistry with lanthanide-organic cages has remained elusive so far. Herein, we report a guest-driven self-assembly and chiral induction approach for the construction of otherwise inaccessible LnL-type (Ln = lanthanide ions, i.

Coordination-Directed Self-Assembly of Functional Polynuclear Lanthanide Supramolecular Architectures.

Lanthanide supramolecular chemistry is a fast growing and intriguing research field due to the unique photophysical, magnetic, and coordination properties of lanthanide ions (Ln). Compared with the intensively investigated mononuclear Ln-complexes, polymetallic lanthanide supramolecular assemblies offer more structural superiority and functional advantages. In recent decades, significant progress has been made in polynuclear lanthanide supramolecules, varying from structural evolution to luminescent and magnetic functional materials.

[Modeling spatial distributions of hydrogen and oxygen stable isotopes in surface soils of the upper reaches of Minjiang River based on machine languages].

To study the feasibility of simulating the spatial distribution of hydrogen and oxygen stable isotopes composition (δH and δO) in the surface soil based on the machine learning method and to investigate large-scale distribution of δH and δO in the upper reaches of Minjiang River, 183 soil samples were collected from the 0-10 cm soil layer. After variable selection, back propagation (BP) neural network, random forests (RF) and support vector machine (SVM) were used to model the δH and δO of the study area, with the accuracies being evaluated. The structural equation model (SEM) was used to reveal the mechanism between the auxiliary variables and the δH and δO of soil water.

Coordination-Assembly of Lanthanide Supramolecular Hydrogels with Luminescent Multi-stimulus Response.

Luminescent supramolecular hydrogels have shown extensive potential for a variety of applications due to their unique optical properties and biocompatibility. Coordination self-assembly provides a promising strategy for the preparation of supramolecular hydrogels. In this contribution, a series of luminescent lanthanide (Ln) supramolecular hydrogels HG-LnL are synthesized by coordination self-assembly of Ln ions and V shaped bis-tetradentate ligands (HL and HL) with different bent angles (∠).

Base- and Metal-Dependent Self-Assembly of Lathanide-Organic Coordination Polymers or Macrocycles with Tetradentate Acylhydrazone-based Ditopic Ligands.

Herein, we report a comprehensive study on the lanthanide-directed coordination self-assembly with two bis-tetradentate acylhydrazone ligands H L and H L . Multifarious outcomes, which are base- and metal-dependent, were revealed by NMR, ESI-TOF-MS and X-ray crystallography. In the absence of base, bent H L was assembled into dinuclear double-strand helicate Ln (H L ) by partially-deprotonated assembly with La, Sm or Eu, while trinuclear Ln (H L ) with Yb or Lu.

Self-Assembly of Lanthanide-Covalent Organic Polyhedra: Chameleonic Luminescence and Efficient Catalysis.

A series of multinuclear lanthanide-covalent organic polyhedra (LnCOPs), including pillar-typed triangular prisms and tetrahedra (Ln = La, Sm, Eu), have been constructed for the first time, through either one-pot subcomponent self-assembly or postassembly metalation. In contrast to the known tetrahedral cages based on transition metals, the pillar-typed polyhedra were favored from the same organic components in the presence of lanthanides. Besides this, facile transmetalations between the polyhedra endow cascade chameleonic luminescence.

Coordination-Assembled Water-Soluble Anionic Lanthanide Organic Polyhedra for Luminescent Labeling and Magnetic Resonance Imaging.

Lanthanide-containing functional complexes have found a variety of applications in materials science and biomedicine because of their unique electroptical and magnetic properties. However, the poor stability and solubility in water of multicomponent lanthanide organic assemblies significantly limit their practical applications. We report here a series of water-stable anionic LnL-type ( = 2, 3, 4, and 5) lanthanide organic polyhedra (LOPs) constructed by deprotonation self-assembly of three fully conjugated ligands (HL and HL) featuring a 2,6-pyridine bitetrazolate chelating moiety.

Metal ion adaptive self-assembly of photoactive lanthanide-based supramolecular hosts.

A post-synthetic transmetallation self-assembly strategy was developed for the preparation of near infrared (NIR) emitting YbL cubes, which could not be synthesized through direct metal-ligand assembly procedures. Metal-adaptive critical structural transformations from LaL triangular prism to LnL cubes were observed along the lanthanide series, the latter of which showed size-selective guest binding behaviour toward poly-aromatic hydrocarbon (PAH) molecules.

The Similarity Between Chinese Five-Pattern and Eysenck's Personality Traits: Evidence From Theory and Resting-State fMRI.

Chinese five-pattern and Eysenck's personality traits are two types of personality theories based on different cultural backgrounds. The former is an indigenous theory, and the latter is a cross-cultural theory. In order to verify the relationship between two different personality traits from theory and neuropsychology, the current study recruited 170 healthy adults to calculate their five-Pattern Personality Inventory (FPPI) and Eysenck Personality Questionnaire-Revised (EPQ) scales and to scan their brains using functional magnetic resonance imaging (fMRI).

Chiral auxiliary and induced chiroptical sensing with 5d/4f lanthanide-organic macrocycles.

A stereocontrolled self-assembly of 5d/4f heterometal-organic macrocycles has been realized by a post-assembly chiral auxiliary approach. Interligand π-π stacking interactions promoted chiral transfer from point-chirality of the auxiliary ligand to metal centers and then the helicates to produced CD and CPL active europium assemblies, facilitating a feasible enantiomeric excess determination by induced chiroptical signals.

A supramolecular lanthanide separation approach based on multivalent cooperative enhancement of metal ion selectivity.

Multivalent cooperativity plays an important role in the supramolecular self-assembly process. Herein, we report a remarkable cooperative enhancement of both structural integrity and metal ion selectivity on metal-organic ML tetrahedral cages self-assembled from a tris-tridentate ligand (L) with a variety of metal ions spanning across the periodic table, including alkaline earth (Ca), transition (Cd), and all the lanthanide (Ln) metal ions. All these ML cages are stable to excess metal ions and ligands, which is in sharp contrast with the tridentate (L) ligand and bis-tridentate (L) ligand bearing the same coordination motif as L.

SIRT1 inhibitory compounds from the roots of Codonopsis pilosula.

Three new compounds, pilosulinene A (1), pilosulinols A (2), and B (3), along with seven known compounds, were isolated from the roots of Codonopsis pilosula cultivated in Xundian County of Yunnan Province. The structures of new compounds were established by spectroscopic methods. In particular, the presence of an aromatic ring in the structure of 1 makes it intriguing.

Cytotoxic and renoprotective diterpenoids from Clerodendranthus spicatus.

Three new diterpenoids, spicatusenes A-C (1-3), and eleven known ones (4-14) were isolated from the aerial parts of Clerodendranthus spicatus. Their structures were identified by spectroscopic methods. The cytotoxic activities of all the compounds against human cancer cells (HL-60, SMMC-7721, A-549, MCF-7, and SW-480) were examined and found that compounds 2, 3, 6, 8, 10, and 13 were active against one or more cancer cell lines.