MicroRNA-146a regulates the production of cytokines in lymphocytes stimulated by lipopolysaccharide.

Objectives: This study aimed to investigate the effects of microRNA-146a (miR-146a) on the production of cytokines in lymphocytes stimulated by lipopolysaccharide (LPS).

Methods: Lymphocytes were harvested from mouse spleen and cultured . The cells were treated with LPS, miR-146a mimic, or miR-146a inhibitor.

Potential association between Fusobacterium nucleatum enrichment on oral mucosal surface and oral lichen planus.

Objective: We determined the bacterial community structure of the buccal mucosa in patients with oral lichen planus (OLP) and evaluated the potential association of Fusobacterium nucleatum with OLP.

Subjects And Methods: We collected buccal mucosal swab samples of patients with OLP (n = 20) and healthy controls (n = 10) and performed 16S rRNA gene sequencing and real-time PCR to determine potentially different bacteria. Damaged and adjacent non-damaged mucosal swab samples of 25 OLP patients were used to detect the amount of F.

Scattering center models of backscattering waves by dielectric spheroid objects.

Scattering center models provide a simple and effective way of describing the complex electromagnetic scattering phenomena of targets and have been successfully applied in radar applications. However, the existing models are limited to conducting objects. Numerical results show that scattering centers of dielectric objects are far more complex than conducting objects and most of them are distributed beyond the object.

[In vitro study on the amelioration of T cell-mediated osteoclastogenesis by anti-RANKL-polyclonal antibody].

Purpose: To characterize the effect of receptor activator of NF-kB ligand (RANKL) polyclonal antibody on T cell-mediated osteoclasogenesis.

Methods: Rabbit anti-rat RANKL polyclonal IgG antibody was performed, performing recombinated mouse RANKL-induced osteoclastogenesis in different antibody doses (1microg/mL,5microg/mL and 10microg/mL), the number of TRAP positive multinuclear cells (cells/well) was calculated under microscopy. The cervical lymph nodes in rats were enriched T-cells for in vitro experiments.

[The effect of B lymphocytes on immune response of periodontal bacteria and osteoclast differentiated reactor-RANKL expression].

Purpose: To study whether B lymphocytes involve in bone resorption in periodontal disease by evaluating RANKL expression of B lymphocytes in immune response to aggregatibacter actinomycetemcomitans (Aa).

Methods: The expression of mRNA transcripts involved in day 1 and day 7 responses of cytokines in cultured rat splenocytes was determined by RT-PCR; The percentage of RANKL-expressing IgG positive cells by B cells in cultured rat splenocytes was measured by flow cytometry; and B lymphocytes capacity for induction of osteoclast differentiation was evaluated by TRAP staining. SPSS 10.