[Extraction, selenium-nanoparticle preparation and anti-virus bioactivity determination of polysaccharides from Caulerpa taxifolia].

Objective: To extract polysaccharides from Caulerpa taxifolia and determine its anti-virus bioactivity.

Methods: The crude polysaccharides were extracted by hot water and precipitated with ethanol, then they were further purified by DEAE-Cellulose and Sephadex G-200 chromatography. After determined by chemicophysical analysis, the polished polysaccharides were selenium treated and bio-assayed by MT and CPE methods.

[Molecular imaging for PET-CT reporter gene in breast adenocarcinoma (HSV1-tk) of subcutaneous xenografts in living nude mice].

Objective: To study the in vitro accumulation of (18)F-FHBG, its in vivo distribution and (18)F-FHBG PET-CT imaging for reporter gene (HSV1-tk) in nude mice with a xenograft of breast adenocarcinoma.

Methods: The in vitro uptake of (18)F-FHBG in tumor cells of T47D and T47D-tk and the distribution of (18)F-FHBG in normal Kunming mice and nude mice with breast adenocarcinoma xenograft were detected by well-type gamma counter. Reporter gene PET-CT imaging with (18)F-FHBG was performed in nude mice with a xenograft of breast adenocarcinoma.

[The study on the extraction and the antivirus activity of amylose extracted from Grateloupia filicina].

Objective: To determine the activity of anti-Herpes simplex II virus (HSV-2) of amylose extracted from Grateloupia filicina.

Methods: Grateloupia filicina amylose was extracted by five kinds of abstraction methods and its suppression on Herpes simplex II virus was observed on cell level in three aspects: the drug activity of protecting cell, the drug influence of HSV-2 proliferation and the drug synthesis action of HSV-2.

Results: Grateloupia filicina had prominence anti-Herpes simplex II virus activity, IC50 of amylose extracted by water distilling and ethanol sedimentation was 5.