Development of polymorphic microsatellite markers for Fagus pashanica (Fagaceae) using next-generation sequencing.

Fagus pashanica is an endangered and endemic tree species in China. To understand its genetic diversity and structure for effective conservation, we used next-generation sequencing data to develop a set of microsatellite markers. Twenty-three of the 68 designed loci were successfully amplified.

Corrigendum: Prevalence and outcomes of pancreatic enzymes elevation in patients with COVID-19: a meta-analysis and systematic review.

[This corrects the article DOI: 10.3389/fpubh.2022.

Comparison of blood loss between tranexamic acid-soaked absorbable Gelfoam and topical retrograde injection via drainage catheter plus clamping in cervical laminoplasty surgery.

Background: To compare the safety and efficacy of tranexamic acid (TXA)-soaked absorbable Gelfoam and the retrograde injection of TXA through a drain with drain-clamping in degenerative cervical laminoplasty patients.

Methods: Patients were assigned into either TXA retrograde injection (TXA-RI), TXA-soaked absorbable Gelfoam (TXA-Gel), or control groups. The demographics, operative measurements, volume and length of drainage, length of hospital stay, complete blood cell count, coagulopathy, postoperative complications, and blood transfusion were recorded.

Self-assembly-induced luminescence of Eu-complexes and application in bioimaging.

Design and engineering of highly efficient emitting materials with assembly-induced luminescence, such as room-temperature phosphorescence (RTP) and aggregation-induced emission (AIE), have stimulated extensive efforts. Here, we propose a new strategy to obtain size-controlled Eu-complex nanoparticles (Eu-NPs) with self-assembly-induced luminescence (SAIL) characteristics without encapsulation or hybridization. Compared with previous RTP or AIE materials, the SAIL phenomena of increased luminescence intensity and lifetime in aqueous solution for the proposed Eu-NPs are due to the combined effect of self-assembly in confining the molecular motion and shielding the water quenching.

[Understanding of the theories of , and in the correlation with meridians and organs].

Based on the original text of (), the theories of , and are analyzed deeply and the practical significance is explored on the correlation with meridians and organs. , and represent respectively the relevant rules between the body surface and internal organs in different patterns and they are the foundation of the multiple correlations of the upper and the lower, the internal and the external and the horizontal and the transverse between meridians and organs. The understanding above not only contributes to the recognition and the exploration of the relevant essence of meridians and organs, but also provides the theoretic guidance to the acupoint selection, diagnosis and treatment of disorders in clinical practice.

Management of Medial Collateral Ligament Insufficiency During Total Knee Arthroplasty with a Screw and Rectangular Spiked Washer: A Case Series of 14 Patients.

Objective: To describe the technique of primary repair of medial collateral ligament (MCL) insufficiency using a screw and rectangular spiked washer in a case series of 14 patients.

Methods: Fourteen patients undergoing MCL repair by a screw and rectangular spiked washer during TKA between March 2018 and March 2019 were retrospectively reviewed. Among them, half injuries were avulsion of the femoral origin, and the other half were MCL laxity.

Composition-Engineered Metal-Organic Framework-Based Microneedles for Glucose-Mediated Transdermal Insulin Delivery.

Elaborately designed glucose-responsive insulin-delivery systems are highly desirable for the treatment of diabetes because it can secrete insulin depending on blood glucose levels. Herein, mimic multi-enzyme metal-organic framework (MOF)-based (insulin and glucose oxidase-loaded cobalt-doped ZIF-8, abbreviated as Ins/GOx@Co-ZIF-8) stimuli-responsive microneedles (MNs) were designed for painless glucose-mediated transdermal administration. In this work, GOx and Co ions were engineered into MOFs to construct a mimic multi-enzyme vehicle.

Smart MMP2-Responsive Nanoprobe for Activatable Fluorescence Imaging-Guided Local Triple-Combination Therapies with Single Light.

Elaborately designed stimuli-responsive smart systems simultaneously enabling activatable imaging and selective treatment are highly desirable for precise diagnosis and therapy of cancer. Herein, such a smart theranostic nanoprobe composed of hollow gold nanospheres (HAuNs), photosensitizer (PS), matrix metalloproteinase 2 (MMP2) substrate peptide, and model drug doxorubicin (DOX) was designed. In the design, HAuNs served as the acceptor of Förster resonance energy transfer (FRET), photothermal therapy (PTT) reagent, and nanocarrier.

Eu/Eu-Based Smart Duplicate Responsive Stimuli and Time-gated Nanohybrid for Optical Recording and Encryption.

With the rapid development of information science, it is urgent that memory devices possessing high security, density, and desirable storage ability should be developed. In this work, a smart duplicate response of stimuli has been developed and a time-gate nanohybrid based on variable valence Eu/Eu coencapsulated has been fabricated and acts as active material in the multilevel and multidimensional memory devices. The luminescence lifetime of Eu in this nanohybrid gave a stimuli response due to which the energy level of the coordinated ligand could be modulated.

Synergistic antiviral effect of curcumin functionalized graphene oxide against respiratory syncytial virus infection.

The diseases attributable to viruses remain a global burden. The respiratory syncytial virus (RSV), which is considered as the major viral pathogen of the lower respiratory tract of infants, has been implicated in severe lung disease. In this contribution, we developed a β-cyclodextrin (CD) functionalized graphene oxide (GO) composite, which displayed excellent antiviral activity and could load curcumin efficiently.

Nonstoichiometric copper chalcogenides for photo-activated alkyne/azide cycloaddition.

Nonstoichiometric copper chalcogenides with heavy copper vacancies can be used as an effective photo-activated catalyst for the Huisgen [3+2] cycloaddition reaction as Cu(i) can be released corresponding to holes (Cu-defects) under light irradiation. These strategies expand new possibilities for carrying out prototypical click chemistry in the presence of functional groups.

"Click" on Alkynylated Carbon Quantum Dots: An Efficient Surface Functionalization for Specific Biosensing and Bioimaging.

Surface functionalization is an essential pre requisite for wide and specific applications of nanoparticles such as photoluminescent (PL) carbon quantum dots (CQDs), but it remains a major challenge. In this report, alkynylated CQDs, prepared from carboxyl-rich CQDs through amidation with propargylamine in the presence of 1,1'-carbonyldiimidazole, were modified efficiently with azido molecular beacon DNA through a copper(I)-catalyzed alkyne-azide cycloaddition reaction (CuAAC). As a proof-of-concept, the DNA-modified CQDs are then bonded with gold nanoparticles (AuNPs, 5 nm) through a gold-sulfur bond.

Optimal postoperative nutrition support for patients with gastrointestinal malignancy: A systematic review and meta-analysis.

Objective: To improve clinical outcomes, parenteral nutrition, standard enteral nutrition and immuno-enhanced nutrition are widely used in the gastrointestinal tumor patients undergoing surgery, but the optimal management of postoperative nutrition support remains uncertain.

Methods: We systematically searched the PUBMED, EMBASE and CNKI to identify latent studies which the effects of standard EN compared with PN or IEN on gastrointestinal tumor patients until the end of November, 2015. The quality of included trials was assessed according to the handbook for Cochrane reviewer.

Curcumin modified silver nanoparticles for highly efficient inhibition of respiratory syncytial virus infection.

Interactions between nanoparticles and viruses have attracted increasing attention due to the antiviral activity of nanoparticles and the resulting possibility to be employed as biomedical interventions. In this contribution, we developed a very simple route to prepare uniform and stable silver nanoparticles (AgNPs) with antiviral properties by using curcumin, which is a member of the ginger family isolated from rhizomes of the perennial herb Curcuma longa and has a wide range of biological activities like antioxidant, antifungal, antibacterial and anti-inflammatory effects, and acts as reducing and capping agents in this synthetic route. The tissue culture infectious dose (TCID50) assay showed that the curcumin modified silver nanoparticles (cAgNPs) have a highly efficient inhibition effect against respiratory syncytial virus (RSV) infection, giving a decrease of viral titers about two orders of magnitude at the concentration of cAgNPs under which no toxicity was found to the host cells.

DNA-AuNP networks on cell membranes as a protective barrier to inhibit viral attachment, entry and budding.

Viral infections have caused numerous diseases and deaths worldwide. Due to the emergence of new viruses and frequent virus variation, conventional antiviral strategies that directly target viral or cellular proteins are limited because of the specificity, drug resistance and rapid clearance from the human body. Therefore, developing safe and potent antiviral agents with activity against viral infection at multiple points in the viral life cycle remains a major challenge.

In situ labelling chemistry of respiratory syncytial viruses by employing the biotinylated host-cell membrane protein for tracking the early stage of virus entry.

An in situ labelling strategy was proposed to produce quantum dot-labelled respiratory syncytial viruses (RSVs) by incorporating the biotinylated membrane protein of the host cells into mature virions, followed by conjugation with streptavidin modified quantum dots (SA-QDs), which has the advantages such as convenience, efficiency and minor influence on viral infectivity and thus could be successfully applied to track the early stage of virus entry.

Real-time imaging of intracellular drug release from mesoporous silica nanoparticles based on fluorescence resonance energy transfer.

In this study, a targeted cancer therapy imaging and sensing system is designed based on doxorubicin (DOX)-loaded green fluorescent mesoporous silica nanoparticles (FMSN) conjugated with folic acid (FA), by linking with α-amine-ω-propionic acid hexaethylene glycol (NH-PEG-COOH). An in situ formation method is adopted to prepare luminescent MSNs, which then act as the donor for the fluorescence resonance energy transfer (FRET), as their emission at 500 nm overlaps with the absorption of the acceptor DOX at 485 nm. NH-PEG-COOH is conjugated to the outer surface of the FMSN at one end and modified by folic acid at the other, so that the formed mesoporous silica composite has the merits of fluorescence imaging, mesoporous nanostructure for drug loading, receptor-mediated targeting and real-time monitoring of intracellular drug release.

Poly[(μ3-3,5-diisopropyl-4H-1,2,4-triazolato-κ(3) N:N':N'')silver(I)].

In the polymeric title compound, [Ag(C8H14N3)] n , the Ag(I) cation is coordinated by three N atoms from three 3,5-diisopropyl-1,2,4-triazolate anions in a T-shaped geometry. The Ag(I) cation deviates from the coordination plane by 0.014 (1) Å and the N-Ag-N bond angles are 96.

A twofold interpenetrating three-dimensional zinc-organic framework built from naphthalene-1,4-dicarboxylate and 4,4'-bipyridine ligands.

A novel three-dimensional Zn(II) complex, poly[[(μ2-4,4'-bipyridine)(μ4-naphthalene-1,4-dicarboxylato)(μ2-naphthalene-1,4-dicarboxylato)dizinc(II)] dimethylformamide monosolvate monohydrate], {[Zn2(C12H6O4)2(C10H8N2)]·2C3H7NO·H2O)}n, has been prepared by the solvothermal assembly of Zn(NO3)·6H2O, naphthalene-1,4-dicarboxylic acid and 4,4'-bipyridine. The two crystallographically independent Zn atoms adopt the same four-coordinated tetrahedral geometry (ZnO3N) by bonding to three O atoms from three different naphthalene-1,4-dicarboxylate (1,4-ndc) ligands and one N atom from a 4,4'-bipyridine (bpy) ligand. The supramolecular secondary building unit (SBU) is a distorted paddle-wheel-like {Zn2(COO)2N2O2} unit and these units are linked by 1,4-ndc ligands within the layer to form a two-dimensional net parallel to the ab plane, which is further connected by bpy ligands to form the three-dimensional framework.

One-step prepared fluorescent copper nanoclusters for reversible pH-sensing.

A one-step synthesis of water soluble and pH-responsive trypsin-stabilized fluorescent copper nanoclusters (CuNCs) was reported without using additional protective or reducing agents, and the as-prepared CuNCs exhibited highly stable properties including oxidation resistance, thermal stability and photostability.

Real-time light scattering tracking of gold nanoparticles- bioconjugated respiratory syncytial virus infecting HEp-2 cells.

Real-time tracking of virus invasion is crucial for understanding viral infection mechanism, which, however, needs simple and efficient labeling chemistry with improved signal-to-noise ratio. For that purpose, herein we investigated the invasion dynamics of respiratory syncytial virus (RSV) through dark-field microscopic imaging (iDFM) technique by using Au nanoparticles (AuNPs) as light scattering labels. RSV, a ubiquitous, non-segmented, pleiomorphic and negative-sense RNA virus, is an important human pathogen in infants, the elderly, and the immunocompromised.

Rapid synthesis of highly luminescent and stable Au20 nanoclusters for active tumor-targeted imaging in vitro and in vivo.

Rapid synthesis of protein-stabilized Au20 nanoclusters (Au20NCs) with high fluorescence quantum yield (QY) up to ∼15% is successfully achieved by manipulating the reaction kinetics. The as-obtained Au20NCs, identified by mass spectrometry, have an average size of 2.6 nm, with strong fluorescence emission at 620 nm (2.