Ketamine inhibits proliferation of neural stem cell from neonatal rat hippocampus in vitro.

Background/aims: Ketamine is a widely used anesthetic in obstetric and pediatric anesthesia. In the developing brain, the widespread neuron apoptosis triggered by ketamine has been demonstrated. However, little is known about its effect on neural stem cells (NSCs) function.

[Clinical features and management of pyothorax due to postoperative cervical anastomotic leakage in esophageal cancer surgery].

Objective: To investigate the clinical characteristics and managements of pyothorax due to postoperative cervical anastomotic leakage after esophageal cancer surgery.

Methods: From January 2006 to January 2013, 3342 patients with esophageal carcinoma underwent esophagectomy and cervical esophagogastric anastomosis. Of them, 19 patients developed pyothorax following cervical anastomotic leakage and their clinicopathological data were analyzed retrospectively.

[Gene expression profiling study of HPV-16 positive and HPV-negative esophageal squamous cell carcinoma].

Objective: To study the difference in gene expression between human papillomavirus (HPV)16-positive and HPV-negative esophageal squamous cell carcinoma(ESCC) .

Methods: Eight HPV 16-positive and seven HPV-negative ESCC specimens were evaluated by PCR. The samples were then determined for gene expression profiling using Solexa Sequencing Chip followed by bioinformatics analysis.

Matrix metalloproteinases expression correlates with survival in patients with esophageal squamous cell carcinoma.

Objectives: The matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases capable of degrading the extracellular matrix and play important roles in malignancies. We evaluated the expression of four MMPs in esophageal squamous cell carcinoma (ESCC), and assessed the association between MMP expression and clinicopathologic characteristics and disease-free survival time.

Methods: We evaluated MMP1, MMP7, MMP9, and MMP13 expression in tissues from 208 patients with ESCC using immunohistochemistry (IHC), and correlated MMP expression to clinicopathologic characteristics and disease-free survival time.

Expression of 11 HOX genes is deregulated in esophageal squamous cell carcinoma.

Purpose: HOX genes are vital for all aspects of mammalian growth and differentiation, and recent data have shown that their deregulated expression is related to carcinogenesis. To date, there has been no systemic study on expression of HOX genes in esophageal carcinoma. We investigated the expression pattern of 39 known HOX genes in cancerous and noncancerous tissue from 36 patients with esophageal squamous cell carcinoma to determine whether their expression is altered in esophageal cancer.

Congenital expression of mdr-1 gene in tissues of carcinoma and its relation with pathomorphology and prognosis.

AIM:To detect the congenital expression patterns of mdr-1 gene in commonly encountered malignant tumors in clinic, and the relationship between the expression of mdr-1 gene and the prognostic morphology in esophageal carcinomas.METHODS:A total of 151 resected samples of malignant tumors without preoperative treatment were taken from Anyang City Tumor Hospital.The congenital expression of their mdr-1 gene was detected with reverse transcription polymerase chain reaction (RT-PCR) and was compared with each other.