[Study on the relationship between partial deletions in the azzospermia factor C region of Y chromosome and male infertility].

Objective: To investigate the relationship between the partial deletions in the azzospermia factor(AZFc) region of Y chromosome and male infertility.

Methods: Multiplex PCR technology was performed to screen the partial deletions in the AZFc region in 158 azoospermia, 160 severe oligozoospermia and 135 oligozoospermia patients and 236 men with normal spermatogenesis.For samples with gr/gr, b2/b3 recombinogenic deletions, author applied RFLP method to identify which DAZ gene doublet deletion was involved.

[Using multiplex PCR to analyze the breakpoint of a severe Y-chromosome deletion].

Objective: To elucidate the relationship between azoospermia factor(AZF) microdeletion of Y chromosome and azoospermia, the exact breakpoint of a severe Y-chromosome deletion was determined according to the physical map of AZF.

Methods: Multiplex polymerase chain reaction was used to amplify fifteen sequence tagged sites (STS), namely sY82, sY84, sY86 in AZFa, sY124, sY127, sY128, sY133, sY134, sY143 in AZFb, sY239, sY242 sY254, sY255 in AZFc, and sY145, sY152 in AZFd; sex-determining region Y(SRY) was taken as an internal control. And then sY82,sY86,sY85,sY84 were further analyzed using the sample of the patient who had Y-chromosome deletion by G band analysis to map the breakpoint at molecular level.

[A genetic study on microdeletion of azoospermia factor region on Y chromosome of azoospermia and oligozoospermia patients].

Objective: To investigate the genetic causes of azoospermia and severe oligozoospermia.

Methods: Cytogenetic analysis and multiplex polymerase chain reaction(PCR) analysis were done on the 148 patients with azoospermia and serious oligozoospermia.

Results: Eleven of the 148(7.

[A cytogenetic and molecular genetic study on microdeletion of AZF region on Y chromosome].

Objective: To study the morphology of Y chromosome and microdeletion of the correlated specific azoospermia factor(AZF) region on Y chromosome in cases of azoospermia and to identify the genetic diagnosis made for male infertility patients.

Methods: Peripheral blood samples were taken from two patients with azoospermia, and then were examined by use of G banding, C banding cytogenetic analysis and multiplex polymerase chain reaction (PCR) microdeletion analysis.

Results: The karyotypes of the two cases were 45, X, -Y, -22, +der(Y)t(Y;22)(q11.