Positive effect of microvascular proliferation on functional recovery in experimental cervical spondylotic myelopathy.

Background And Purpose: Cervical Spondylotic Myelopathy (CSM), the most common cause of spinal cord dysfunction globally, is a degenerative disease that results in non-violent, gradual, and long-lasting compression of the cervical spinal cord. The objective of this study was to investigate whether microvascular proliferation could positively affect neural function recovery in experimental cervical spondylotic myelopathy (CSM).

Methods: A total of 60 male adult Sprague-Dawley (SD) were randomly divided into four groups: Control (CON), Compression (COM), Angiostasis (AS), and Angiogenesis (A G),with 15 rats in each group.

Neurovascular Unit Compensation from Adjacent Level May Contribute to Spontaneous Functional Recovery in Experimental Cervical Spondylotic Myelopathy.

The progression and remission of cervical spondylotic myelopathy (CSM) are quite unpredictable due to the ambiguous pathomechanisms. Spontaneous functional recovery (SFR) has been commonly implicated in the natural course of incomplete acute spinal cord injury (SCI), while the evidence and underlying pathomechanisms of neurovascular unit (NVU) compensation involved in SFR remains poorly understood in CSM. In this study, we investigate whether compensatory change of NVU, in particular in the adjacent level of the compressive epicenter, is involved in the natural course of SFR, using an established experimental CSM model.

Ultrastructural destruction of neurovascular unit in experimental cervical spondylotic myelopathy.

Background And Purpose: The pathogenesis of cervical spondylotic myelopathy (CSM) remains unclear. This study aimed to explore the ultrastructural pathology of neurovascular unit (NVU) during natural development of CSM.

Methods: A total of 24 rats were randomly allocated to the control group and the CSM group.

[Identification and evaluation of Citrus grandis based on DNA barcode,UPLC and chromaticity method].

In order to identify the source of Citrus grandis and evaluate its quality originate from two areas comprehensively,DNA barcode was used to identify 26 samples of C. grandis. The content of naringin,rhoifolin,naringenin and apigenin was determined by UPLC method,and the color difference was numerically studied by color difference analyzer,which was related to the effective components of C.

Limb remote ischemic postconditioning protects integrity of the blood-brain barrier after stroke.

Integrity of the blood-brain barrier structure is essential for maintaining the internal environment of the brain. Development of cerebral infarction and brain edema is strongly associated with blood-brain barrier leakage. Therefore, studies have suggested that protecting the blood-brain barrier may be an effective method for treating acute stroke.

[Mechanisms of Hirudo in promoting blood circulation and removing stasis based on network pharmacology].

The study aims to analyze the mechanisms of Hirudo in promoting blood circulation and removing blood stasis based on network pharmacology. A database of chemical components of Hirudo was established through literature retrieval. The targets were predicted by using the reverse pharmacophore matching method and screened according to the antithrombotic and anticoagulant drug targets approved by FDA in the DrugBank database.

Effect of acrylamide-induced neurotoxicity in a primary astrocytes/microglial co-culture model.

Acrylamide (AA), is a common food contaminant generated by heat processing. Astrocytes and microglia are the two major glial cell types in the brain that play pivotal but different roles in maintaining optimal brain function. The objective of this study is to investigate the neurotoxicity of AA, using a primary astrocytes/microglia co-culture model.

Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression.

Background: Bone marrow mesenchymal stem cell (BMSCs)-based therapy seems to be a promising treatment for acute lung injury, but the therapeutic effects of BMSCs transplantation on acute lung injury induced by brain ischemia and the mechanisms have not been totally elucidated. This study explores the effects of transplantation of BMSCs on acute lung injury induced by focal cerebral ischemia and investigates the underlying mechanism.

Methods: Acute lung injury model was induced by middle cerebral artery occlusion (MCAO).

Membrane Damage Induced by Supercritical Carbon Dioxide in Rhodotorula mucilaginosa.

To clarify the mechanism of microbial inactivation by supercritical carbon dioxide (SCCO2), membrane damage of Rhodotorula mucilaginosa was investigated within specific pressure (10 Mpa), temperature (37 °C), and treatment time (10-70 min) ranges, including cell morphological structure, membrane permeability and fluidity. SEM and TEM observations showed morphological changes in the cell envelope and intracellular organization after SCCO2 treatment. Increase of membrane permeability was measured as increased uptake of the trypan blue dye with microscopy, and leakage of intracellular substances such as UV-absorbing materials and ions by determining the change of protein and electrical conductivity.

Inactivation of polyphenol oxidase from watermelon juice by high pressure carbon dioxide treatment.

The inactivation of polyphenol oxidase from watermelon juice with high pressure carbon dioxide (HPCD) treatment was investigated. The maximum reduction of polyphenol oxidase (PPO) activity inactivated by HPCD treatment was 95.8% at 30 MPa and 50 °C for 30 min, which was far higher than 50.

Physico-chemical and antioxidant properties of four mango (Mangifera indica L.) cultivars in China.

Four principal mango cultivars (Tainong No.1, Irwin, JinHwang and Keitt) grown in southern China were selected, and their physico-chemical and antioxidant properties were characterized and compared. Of all the four cultivars, Tainong No.

[X-ray diffraction study of high hydrostatic pressure on crystalline structure of different type starches].

Crystalline changes of different type starches after high hydrostatic pressure treated under 300, 450, 600 MPa were studied by X-ray diffraction. Waxy maize (A type, 100% amylopectin), hylon VII (B type, 30% amylopectin) and tapioca starch (C type, 83% amylopectin) were chosen. The results indicated that for waxy maize starch, annealing effect was observed at 300 MPa, disappearance of crystalline structure happened at 450 MPa and retrogradation at 600 MPa.

[Study on the effect of high hydrostatic pressure treatment on the secondary structure of mushroom polyphenoloxidase by SRCD and FTIR].

The secondary structure of the mushroom polyphenoloxidase treated by the high hydrostatic pressure (HHP) was analyzed by the synchrotron radiation circular dichroism (SRCD) and Fourier transform infrared spectroscopy (FTIR). The alpha-helix content of mushroom PPO was decreased after HHP treatment, which indicated that the secondary structure of PPO was changed. There was a discrepancy of the result of the secondary structure content between untreated or HHP-treated mushroom PPO analyzed by SRCD and FTIR spectra, and this discrepancy may be due to the different determination temperature, the concentration of the PPO solution and the spectra analysis method etc.

Troglitazone induced apoptosis via PPARγ activated POX-induced ROS formation in HT29 cells.

Objective: In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells, the effects of PPARγ and POX-induced ROS were explored.

Methods: [3- (4, 5)-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay, Annexin V and PI staining using FACS, plasmid transfection, ROS formation detected by DCFH staining, RNA interference, RT-PCR & RT-QPCR, and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARγ pathway and POX-induced ROS formation in HT29 cells.

Results: Troglitazone was found to inhibit the growth of HT29 cells by induction of apoptosis.

[Analysis of the extraction of red pellicle of walnut (Juglans regia L.) by ultraviolet-visible spectra and HPLC-ESI-MS(n)].

The extraction of the red pellicle of walnut (Juglans regia L.) was analyzed by UV-visible spectra and HPLC-ESI-MS(n) (high-performance-liquid-chromatography coupled with electrospray ionization mass spectrometry). The extraction in ethanol-HCl showed two absorption peaks at 560 and 591 nm respectively in the UV-Vis spectrum; after purified by lead acetate and thin-layer-chromatography, the extraction in ethanol-HCl showed 4 absorption peaks at 340, 370, 552 and 585 nm respectively.

[Effects of high pulsed electric field on the secondary and tertiary structure of lipoxygenase].

The effect of pulsed electric field (PEF) on the secondary and tertiary structure of lipoxygenase (LOX) in a buffer solution was analyzed using far UV-circular dichroism (CD) and fluorescence spectrophotometry, respectively. The secondary and tertiary structure of LOX changed after PEF treatment. The CD spectra of LOX also changed, with the intensity of two negative peaks and the content of alpha-helix significantly decreased (p < 0.

[Determination of mineral elements in eight pear varieties by ICP-MS after microwave-assisted digestion].

A study was carried out on the contents of mineral elements such as Na, K, Ca, Mg, Zn, Cu, Fe, Mn and B in eight different pear varieties by ICP-MS after microwave-assisted digestion. The results indicated that the main elements of the eight pears are similar, but the content of each element is different. The content of K is the highest in the detected pears, followed by Mg, Ca and Na.

[The characteristic analysis of several mineral contents in Chinese orange juice].

A study was carried out on the contents of mineral elements such as K, Ca, Na, and Mg in seven different orange varieties, namely Pineapple orange, Hamlin, Trovita, Jincheng, 1,232 Tangor, Olinda Valencia and Delta Valencia, by flame atomic absorption spectrometry. The results indicated that the content sequence of different nutritional elements was K > Mg > Ca > Na, with a range of 1,233.75-1,866.

Biological properties of different protein extracts from selenium-enriched Ganoderma lucidum.

Our previous studies showed that Ganoderma lucidum bio-transformed 20-30% of inorganic selenium from substrate to organic forms by preferentially incorporating selenium into proteins. In the present study, four kinds of protein extracts from selenium-enriched G. lucidum were prepared with different extracting solvents, which contain water-soluble, alkaline-soluble, salt-soluble and alcohol-soluble protein extracts, and the effects on antioxidant activity of characterizations of protein extracts were studied.

Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells.

Aim: To investigate the effect of ceramide on the cell cycle in human hepatocarcinoma Bel7402 cells. Possible molecular mechanisms were explored.

Methods: [3- (4, 5)-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay, plasmid transfection, reporter assay, FACS and Western blotting analyses were employed to investigate the effect and the related molecular mechanisms of C2-ceramide on the cell cycle of Bel7402 cells.

Changes in the volatile compounds and chemical and physical properties of Kuerle fragrant pear (Pyrus serotina Reld) during storage.

Volatiles from stored Kuerle fragrant pears (Pyrus serotina Reld) were studied using high-resolution gas chromatography and the solid-phase microextraction (SPME) method of gas chromatography/mass spectrometry (GC/MS). The dominant components were hexanal, ethyl hexanoate, ethyl butanoate, ethyl acetate, hexyl acetate, ethanol, alpha-farnesene, butyl acetate, and ethyl (E,Z)-2,4-decadienoate. By using GC-olfactometry, it demonstrated that the volatile compounds from SPME were responsible for the aroma of the Kuerle fragrant pear.

[Expression of Nestin following focal cerebral ischemia-reperfusion in rats].

Objective: To study the expession of Nestin after focal ischemia-reperfusion in rats.

Methods: The model of acute reperfusion injury after cerebral ischemia in rat was made by middle cerebral artery occlusion. All animals were allowed 2 h ischemia and sacrificed after different durations of reperfusion (6 h, 12 h, 24 h, 72 h, 7 d, 14 d).

[Isolation and identification Alicyclobacillus from concentrated apple juice processing].

The forty-five strains of bacteria have been isolated from concentrated apple juice. All of them can grow at K agar (pH 4.0), but can't grow at LB palate (pH 7.