[A novel fluorophotometric method for assaying free glutathione of both reduced and oxidized in plasma].

To investigate the measurement of free glutathione of both reduced or oxidized (i.e. GSH and GSSG) in plasma, and to evaluate the redox state of GGSH/GSSG in human plasma, both GSH and GSSG in plasma were measured by fluorophotometry based on the facts that one molar GSSG can be reduced to two molar GSH by dithiothreitol(DTT) under the condition of the pH being about 6.

[Superoxide dismutase biosensor for screening substances possessing scavenging superoxide anion activity].

Aim: To establish a method for screening active substance with scavenging effects on superoxide anion in vitro by designed superoxide dismutase biosensor.

Methods: The enzyme sensor was built by connecting the immobilized CuZnSOD with optical oxygen sensor through a special way. Superoxide anions were generated by auto-oxidation of pyrogallol.

[Study on the tumor cell targetability of folate-conjugated albumin nanoparticles].

Objective: To study folate-conjugated albumin nanoparticles targeting to tumor cells via folate receptor-mediated endocytosis.

Methods: The activated folic acid (N-hydroxysuccinimide ester of folic acid) was conjuated to the surface of bovine serum albumin nanoparticles (BSANP) via the amino groups. The extent of the influence that concentration, incubation time and free folate exerted on the uptake of Folate-BSANP by ovarian cancer cells (SKOV3) was determined using fluorescence spectrophotometer.

[Preparation and transfection of GFP plasmid DNA/cationic liposome complex].

Objective: This work was directed at obtaining a better gene carrier to improve the effects of gene delivery.

Methods: Cationic liposomes made from cholesterol, lecithin and Eighteenth Amic by reverse evaporation technique were used for encapsulating plasmid DNA containing gfp gene. The DNA/liposome complexes differed in quantity of plasmid DNA.