A novel snake venom C-type lectin-like protein modulates blood coagulation by targeting von Willebrand factor and coagulation factor IX.

Snake venom C-type lectin-like proteins (CLPs) belong to the nonenzymatic proteins. To date, no CLP with both platelet and coagulation factors activating activities has been reported. In this study, a novel CLP, termed protocetin, with molecular weight of 29.

Moyamoya syndrome may result from psoriasis: Four case reports.

Background: Moyamoya syndrome (MMS) is a group of diseases that involves more than one underlying disease and is accompanied by moyamoya vascular phenomena. Psoriasis is a chronic immune skin disease closely linked to high blood pressure and heart disease. However, psoriasis-related MMS has not been reported.

[Determination of eight cannabinoids in foods with enhanced matrix removal-lipid adsorbent by ultra performance liquid chromatography-tandem mass spectrometry].

A novel method was developed for the simultaneous determination of eight cannabinoids in six types of food matrices, including chocolate, fondant, biscuit, beverage, cookie and baijiu, using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The sample extraction and cleanup steps were optimized, and various purification methods were investigated to remove the oil matrix and glue in chocolate and fudge, respectively. Enhanced matrix removal-lipid adsorbent (EMR-Lipid) provided efficient, selective cleanup of the evaluated matrices.

Polyacetylene Isomers Isolated from L. Suppress the Metastasis of Gastric Cancer Cells by Inhibiting Wnt/-Catenin and Hippo/YAP Signaling Pathways.

()-7-Phenyl-2-hepten-4,6-diyn-1-ol () and ()-7-Phenyl-2-hepten-4,6-diyn-1-ol () are isomeric natural polyacetylenes isolated from the Chinese medicinal plant L. This study first revealed the excellent anti-metastasis potential of these two polyacetylenes on human gastric cancer HGC-27 cells and the distinctive molecular mechanisms underlying their activities. Polyacetylenes and significantly inhibited the migration, invasion, and adhesion of HGC-27 cells at their non-toxic concentrations in a dose-dependent manner.

[Emission of Nitrous Oxide (NO) from Lake Taihu and the Corresponding Potential Driving Factors].

Nitrous oxide (NO) is one of the six greenhouse gases stipulated in the Kyoto Protocol. Its greenhouse potential over the past century was 298 times that of CO, and the concentration of atmospheric NO has been continuously and rapidly increasing during the past hundred years. Shallow lakes are an important source of atmospheric NO.

[Emission of Methane from a Key Lake in the Eastern Route of the South-to-North Water Transfer Project and the Corresponding Driving Factors].

Lakes play an important role in the biogeochemical cycling of dissolved organic matter (DOM) and the emission of methane (CH). We investigated the concentration and effluxes of CH and then analyzed the corresponding driving factors in Lake Luoma, a key lake along the South-to-North Water Transfer Project. Our results indicated that Lake Luoma was a hotspot of CH emissions with an annual mean concentration and efflux of (0.

[Optical Composition and Potential Driving Factors of Chromophoric Dissolved Organic Matter in Large Lakes and Reservoirs in the Eastern Region of China].

Intensified urbanization has been occurring in the eastern region of China in recent decades, and excessive industrial and household sewage has been discharged into lakes and reservoirs, which has directly lowered water quality and destructed the functions of aquatic ecosystems. Lakes and reservoirs are typically drinking water sources supplying water for metropolitan areas as well as large- and medium-sized cities. Chromophoric dissolved organic matter (CDOM) is the colored fraction of DOM, and its source and optical composition strongly affect water supply safety and the health of surrounding citizens.

[Influences of Hydrological Scenarios on the Bioavailability, Fate, and Balance of Chromophoric Dissolved Organic Matter in Lake Poyang].

Lake Poyang has significant differences in hydrological characteristics between the flood and dry seasons. Unraveling the optical composition, bioavailability, fate, and balance of chromophoric dissolved organic matter (CDOM) and organic carbon fluxes in Lake Poyang under different hydrological conditions can help provide advanced schemes on carbon cycling, the transfer and transformation of organic matter, and water resource management of the lake. Three fluorescent components, including a humic-like (C1), a tryptophan-like (C2), and a tyrosine-like (C3) component, were obtained using three-dimensional fluorescence spectroscopy coupled with parallel factor analysis.

[Source and Optical Dynamics of Chromophoric Dissolved Organic Matter in the Watershed of Lake Qinghai].

Lake Qinghai is the largest lake in China and is of great significance to maintain the ecological security of the Qinghai-Tibet Plateau. Few studies have been carried out to investigate the optical composition and source of chromophoric dissolved organic matter (CDOM) in large lakes on the Qinghai-Tibet Plateau. It is of great significance to study the source and optical dynamics of CDOM in Lake Qinghai watershed for water quality protection and filling in the gaps in the knowledge of CDOM variability in a remote area.

[Sources and Optical Dynamics of Chromophoric Dissolved Organic Matter in Different Types of Urban Water Bodies].

In the past few decades, China's rapid industrial activities and urbanization processes have greatly impacted the urban surface water ecosystem. The changes in the quality of urban surface water directly affect the supply and carbon cycling of urban waters. We collected 50 water samples from urban rivers, lakes, and reservoirs in the city of Changchun in June 2020.

Analysis of the Effect of N555 Mutations on the Product Specificity of Dextransucrase Using Caffeic Acid Phenethyl Ester as an Acceptor Substrate.

Bioglycosylation is an efficient strategy to improve biological activities and physicochemical properties of natural compounds to develop structural modifications of drugs. In this study, an N555 residue was identified as a candidate for site-directed mutagenesis through sequence alignment with GTF180ΔN. Caffeic acid phenethyl ester (CAPE) was used as an acceptor substrate.

Identification of spin effects in the anomalous Righi-Leduc effect in ferromagnetic metals.

The emerging of spin caloritronics leads to a series of new spin-thermal related effects, such as spin Seebeck effect (SSE), spin Nernst effect (SNE) and their corresponding inverse effects. Anomalous Righi-Leduc effect (ARLE) describes that a transverse temperature gradient can be induced by a longitudinal heat flow in ferromagnets. The driving force and the response of the ARLE are all involved with heat.

[Screening of the Optimum Medium for Rat Mesenchymal Stem Cells].

Objective: To investigate the effect of three different cell culture mediums, DMEM-LG, α-MEM and DMEM/F12, on the growth of rat bone marrow mesenchymal stem cells (BMSCs) , and so that to screen out the most suitable medium for culturing the rat BMSCs.

Methods: BMSCS were isolated from the femur and tibia of SD rats by whole bone marrow differential adherence method. The isolated cells were then cultured with three culture mediums, DMEM-LG, α-MEM and DMEM/F12.

Damaged male germ cells induce epididymitis in mice.

Epididymitis can be caused by infectious and noninfectious etiological factors. While microbial infections are responsible for infectious epididymitis, the etiological factors contributing to noninfectious epididymitis remain to be defined. The present study demonstrated that damaged male germ cells (DMGCs) induce epididymitis in mice.

Efficacy of orthodontic and orthognathic treatment for oral and maxillofacial deformities.

Background: This study aims to assess the efficacy and safety of orthodontic and orthognathic treatment (OOT) for patients with oral and maxillofacial deformities (OMDF) systematically.

Methods: This study will comprehensively search Cochrane Library, PubMed, EMBASE, Scopus, Web of Science, PsycINFO, Index to Nursing and Allied Health Literature, Allied and Complementary Medicine Database, Chinese Biomedical Literature Database, and China National Knowledge Infrastructure from their inceptions to the July 1, 2019. Grey literature will be explored via searching dissertations, Google scholar and conference abstracts.

Transglycosylation Improved Caffeic Acid Phenethyl Ester Anti-Inflammatory Activity and Water Solubility by Leuconostoc mesenteroides Dextransucrase.

Bioglycosylation is an efficient strategy to improve the biological activity and physicochemical properties of natural compounds for therapeutic drug development. In this study, two caffeic acid phenethyl ester (CAPE) glucosides (G-CAPE and 2G-CAPE) were synthesized by transglycosylation with dextransucrase from Leuconostoc mesenteroides 0326 with CAPE as an acceptor and sucrose as a donor. The products were purified and the structures were characterized.

Predicting hepatocellular carcinoma development for cirrhosis patients via methylation detection of heparocarcinogenesis-related genes.

Most hepatocellular carcinoma (HCC) patients have undergone a progression from chronic hepatitis, then liver cirrhosis (LC), and finally to carcinoma. The objective of this study was to elucidate risk factors to predict HCC development for cirrhosis patients. Multiple methylated specific PCR (MSP) was applied to determine methylation status of heparocarcinogenesis-related genes in 396 tissue and plasma specimens and multivariate cox model was used to analyze the relationship between risk variables and HCC development among cirrhosis patients, followed up in a median period of 30 months.

[BORIS Regulates SOCS3 Expression Through Epigenetic Mechanisms in Human Hepatocellular Carcinoma Cells].

Objective: To study the regulation of suppressor of cytokine signaling 3 (SOCS3) expression bythe brother of the regulator of the imprinted site (BORIS) in hepatocellular carcinoma cell.

Methods: The expression of mRNA in HCC cell lines was detected by real-time quantitative PCR (qRT-PCR). The expression of SOCS3 protein in knockdown and overexpression of HCC cell lines was tested by Western blot.

[Klf10 silencing inhibited mechanical force induced osteogenic differentiation of human periodontal ligament cells].

Purpose: To investigate the effect of Klf10 silence on human periodontal ligament cells (HPDLCs) under mechanical force.

Methods: HPDLCs were isolated and transfected with Klf10 siRNA, and then exposed to centrifugal force for 6 h at 631 r/min. Purmorphamine, an hedgehog signaling pathway agonist, was used for intervention.

Application of multiplex methylated-specific PCR with capillary electrophoresis to explore prognostic value of TSGs hypermethylation for hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) is a malignant tumor that severely threatens human health. To date, early detection for HCC patients is particularly significant due to their poor survival rates even after liver resection.

Methods: Therefore, an efficient and sensitive detection method for monitoring liver cancer, multiplex methylation-specific PCR (MSP) coupled with capillary electrophoresis, is developed.

[Research on Chemical Constituents and Anti-tumor Activity of Eupatorium chinense].

Objective: To study the chemical constituents and their anti-tumor activity of Eupatorium chinense. Methods: The chemical constituents were separated and purified by the normal phase silica gel column chromatography,preparative thin-layer chromatography,and preparative HPLC. Their structures were determined by various spectral data,their antitumor activity in vitro was determined by MTT assay.

[Cardioprotective Effect and Its Mechanism of Total Saponins of Panacis Majoris Rhizoma in Myocardial Infarction Rats].

Objective: To explore the cardioprotective effect and its mechanism of total saponins of Panacis Majoris Rhizoma in myocardial infarction (MI) rats.

Methods: The MI model rats induced by ligating anterior descending branch of coronary artery were randomly divided into four group:model group, total saponins of Panacis Majoris Rhizoma (100 and 200 mg/kg) groups and compound Danshen dripping pills group. The rats were orally administrated with drugs once a day for four weeks.

Thermally Driven Pure Spin and Valley Currents via the Anomalous Nernst Effect in Monolayer Group-VI Dichalcogenides.

The spin and valley-dependent anomalous Nernst effects are analyzed for monolayer MoS_{2} and other group-VI dichalcogenides. We find that pure spin and valley currents can be generated perpendicular to the applied thermal gradient in the plane of these two-dimensional materials. This effect provides a versatile platform for applications of spin caloritronics.