N-methyladenosine reader YTHDF2-mediated long noncoding RNA FENDRR degradation promotes cell proliferation in endometrioid endometrial carcinoma.

Dysregulation of long noncoding RNA (LncRNA) FENDDR has been shown to be closely related to the progression of several cancers. However, its role and upstream regulatory mechanism in endometrioid endometrial carcinoma (EEC) remains unclear. This study was conducted using the cancerous tissues of EEC patients (n = 60), EEC cell lines, and a xenograft mouse model.

[Stabilities of two kinds of Plasmodium antigen substrate slides under different storage temperatures and time].

Objective: To observe and compare the inspection effects of antigen substrate slides of Plasmodium cynomolgi (P. c) and Plasmodium falciparum (P. f) on the malaria antibody titer under different storage temperatures and time.

[Correlation of IL-4 and IL-13 gene polymorphisms with asthma and total serum IgE levels].

Objective: To investigate the correlation between the single nucleotide polymorphisms (SNP) of +33C/T in the promoter region of IL-4 gene and +1923C/T in intron-3 region of IL-13 gene and the susceptibility of asthma, and to study the impact of these polymorphisms upon total serum IgE levels.

Methods: The 2 polymorphisms were determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), in 150 asthmatic subjects (asthma group) and 160 healthy controls (healthy control group) of the Han nationality in Shandong province enrolled from December 2003 to June 2007. The total serum IgE levels were determined by ELISA.

[Establishment of in vitro microtest for determining sensitivity of Plasmodium falciparum to pyronaridine].

Objective: To establish an in vitro microtest for determining the sensitivity of Plasmodium falciparum to pyronaridine.

Methods: Pyronaridine-coated plate and culture medium which is easy to use in the field were prepared. P.

[The point mutations in Pfcrt and Pfmdr1 genes in Plasmodium falciparum isolated from Hainan Province].

Objective: To evaluate the point mutations in Pfcrt and Pfmdr1 genes in Plasmodium falciparum isolated from Hainan Province.

Methods: Nested polymerase chain reaction and restriction fragment length polymorphism were used to detect the point mutations at codon 76 of Pfcrt and at codon 86, 1246 of Pfmdr1 in P. falciparum isolates.

[Fluctuation in the resistance of Plasmodium falciparum to chloroquine in China].

Objective: To investigate whether chloroquine-resistance of Plasmodium falciparum had changed after stopping or reducing the use of chloroquine as an antimalarial in Hainan and Yunnan provinces.

Methods: WHO standard in vitro microtest and 4-week in vivo test were used, assays were carried out in different time after stopping or reducing the use of chloroquine.

Results: In vitro test in Hainan indicated that the rate of chloroquine-resistant P.

[The correlation between tooth pain and bioactivators changes in gingival crevicular fluid after applying orthodontic stress].

Objective: To investigate the changes of tooth pain in patients with orthodontic tooth movement, to study the changes of bioactivators in GCF in patients with orthodontic tooth movement, and explore the correlation between pain and bioactivators in orthodontic patients.

Methods: 50 patients were included, each having one treatment tooth and one contralateral control tooth. The levels of PGE(2), P substances, IL-6 and GM-CSF in GCF of upper lateral incisor were investigated before activation and at 12th hour, 24th hour, 48th hour and 72th hour after the applying labial orthodontic force, by using highly sensitive radioimmunassay.