Application of fluorescein combined with methylene blue in sentinel lymph node biopsy of breast cancer.

Sentinel lymph node biopsy (SLNB) for axillary lymph node staging in early breast cancer has been widely recognized. The combination of radio-colloids and dye method is the best method recognized. The reagents and equipment required in the process of the combined method are complex and expensive, so there are certain restrictions in the use of primary medical institutions.

Hepatitis E virus re-infection accelerates hepatocellular carcinoma development and relapse in a patient with liver cirrhosis: A case report and review of literature.

Background: Hepatitis E virus (HEV) superinfection is a suspected promoting factor for hepatocellular carcinoma (HCC) in patients with chronic hepatitis and cirrhosis. However, to date, very few cases of HEV-related HCC have been reported. Nevertheless, the role of HEV re-infection in cirrhotic liver without other chronic hepatitis infections has rarely been explored.

Liver stiffness measurements with supersonic shear wave elastography in the diagnosis of biliary atresia: a comparative study with grey-scale US.

Objectives: To prospectively assess the diagnostic performance of supersonic shear wave elastography (SSWE) in identifying biliary atresia (BA) among infants with conjugated hyperbilirubinaemia by comparing this approach with grey-scale ultrasonography (US).

Methods: Forty infants were analysed as the control group to determine normal liver stiffness values. The use of SSWE values for identifying BA was investigated in 172 infants suspected of having BA, and results were compared with the results obtained by grey-scale US.

Case Report of Contrast-Enhanced Ultrasound Features of Primary Hepatic Neuroendocrine Tumor: A CARE-Compliant Article.

Primary hepatic neuroendocrine tumors (PHNETs) are very rare and their clinical features and treatment outcomes are not well understood. It is difficult to reach a proper diagnosis before biopsy or resection. The aim of this study was to analyze the imaging features of PHNETs on contrast-enhanced ultrasound (CEUS).

Randomized, controlled trial comparing the efficacy of intrauterine balloon and intrauterine contraceptive device in the prevention of adhesion reformation after hysteroscopic adhesiolysis.

Objective: To compare the efficacy of heart-shaped intrauterine balloon and intrauterine contraceptive device (IUD) in the prevention of adhesion reformation after hysteroscopic adhesiolysis.

Design: Prospective, randomized, controlled trial.

Setting: University hospital.

Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy.

Background: S100P is a member of the S100 family of calcium-binding proteins, and it participates in pathophysiological events, such as tumor growth and invasion. Based on the striking similarities between trophoblast cells and tumor cells with regard to proliferative and invasive properties, we raised the question of whether and how S100P expresses in trophoblast cells during development. This study aimed to investigate the expression pattern of S100P in the human placenta during pregnancy development.

[Effects of early intervention of second-look office hysteroscopy in the prevention of adhesion reformation for moderate-severe Asherman's syndrome].

Objective: To compare the efficacy of different time interval in the prevention of adhesion reformation after hysteroscopic adhesiolysis for moderate-severe Asherman's syndrome.

Methods: A total of 125 women with moderate-severe Asherman's syndrome undergoing hysteroscopic division of intrauterine adhesion were enrolled into this retrospective cohort study. All patients underwent second-look hysteroscopy after a certain period of the first hysteroscopic adhesiolysis, and the operation would be performed again if any adhesion existed.

[Influencing factors of reproduction status of patients undergoing laparoscopic myomectomy].

Objective: To explore the influencing factors of reproduction status in women undergoing laparoscopic myomectomy (LM).

Methods: A total of 278 LM patients were recruited.We retrospectively reviewed the reproduction status of 87 pregnant cases after LM.

[Changes in peripheral hemogram among workers with short-term lead exposure].

Objective: To examine the effect of short-term occupational lead exposure on the inflammatory response system in blood among workers.

Methods: A total of 255 lead-exposed workers (length of service ≤1 year) at an electronics factory in Dongguan, China (exposure group), as well as 205 managers without any occupational exposure at another factory (control group), were included in the study. Occupational physical examination was done to get peripheral blood counts.

Fertility-preserving treatment and pregnancy outcomes in the early stage of endometrial carcinoma.

Objective: This study aimed to review the available literature on fertility-preserving treatment and pregnancy outcomes in patients with early-stage endometrial carcinoma who desired to preserve their fertility.

Data Sources: The PubMed database (1992-2012) was searched for the words "conservative "OR" fertility sparing "OR" fertility preserving" AND "endometrial neoplasms" (MeSH). All relevant articles in English and the relevant references were collected.

Outcome of conservative treatment and subsequent assisted reproductive technology for patients with early-stage endometrial adenocarcinoma and poor fertility potential.

Conservative treatment with high doses of progestin is an alternative to standard hysterectomy for young patients with early-stage endometrial adenocarcinoma who desire to preserve their fertility. Here we report a patient with well-differentiated early-stage endometrial adenocarcinoma and poor fertility potential who failed to become pregnant in two in vitro fertilization-embryo transfer cycles and suffered a relapse after conservative treatment. This case illustrates that assessment of fertility potential is critical at the time of initial evaluation and conservative treatment planning for patients with endometrial adenocarcinoma.

Outcome of in vitro fertilization in endometriosis-associated infertility: a 5-year database cohort study.

Background: Endometriosis affects natural fertility through various approaches, and in vitro fertilization (IVF) is a good treatment. But the IVF result of endometriosis patients is still under debate. We investigated the effect of endometriosis on IVF by analyzing the data from a single reproductive center.

[Risk factors associated with twin pregnancy in double embryo transfer].

Objective: To explore the risk factors associated with twin pregnancy in double embryo transfer.

Methods: A retrospective analysis was performed for 2970 double embryo transfer cycles, including 1984 cycles of fresh embryo transfer and 986 cycles of frozen-thawed embryo transfer (FET). Multiple factor Logistic regression was used.

[Analysis of low-quality embryo transfer strategy in in vitro fertilization and embryo transfer cycle].

Objective: To investigate the transfer strategy of low-quality embryo in in vitro fertilization and embryo transfer (IVF-ET) cycle.

Methods: A retrospective analysis was performed for the clinical data of 621 IVF-ET cycles under controlled ovarian hyperstimulation, including 574 fresh embryo transfer (ET) cycles (Group T1) and 47 frozen-thawed embryo transfer (FET) as the first ET cycles (Group C1). Logistic regression was used to model the probability of clinical pregnancy rate based on the cycle-specific factors.

[Analysis of risk factors for triplet pregnancy after a simultaneous transfer of triplicate embryos].

Objective: To analyze the risk factors for triplet pregnancy after a simultaneous transfer of triplicate embryos.

Methods: The investigators carried out a retrospective analysis of 769 cycles in which three embryos were transferred in one treatment cycle, including 298 fresh embryo transfer (ET) cycles and 471 frozen-thawed ET (FET) cycles. The impact of patient age and the number of good embryos transferred on the rates of clinical pregnancy and triplet pregnancy was studied according to different cycle types.

Maintenance of early pregnancy without early hormone support after frozen-thawed embryo transfer in hormone replacement treatment cycles.

Objective: To report two successful pregnancies after frozen-thawed ET in discontinued hormone replacement treatment cycles.

Design: Case report.

Setting: University hospital.

[Fertilizing ability, cleavage potential and inheritance risk of globozoospermia].

Objective: To explore the fertilizing ability, cleavage potential and inheritance risks of globozoospermia.

Methods: A globozoospermic patient was diagnosed by sperm morphological staining and transmission electron microscope. From his wife the investigators obtained 26 oocytes in which 6 oocytes were donated and the other 19 (in 20) MII oocytes injected into 19 round-headed sperms.

[Comparison of surgery for staging using laparoscopy and laparotomy in patients with endometrial cancer].

Objective: To compare the surgical outcomes for staging by laparoscopy and laparotomy in patients with endometrial cancer, evaluate the feasibility of laparoscopic surgical treatment of endometrial cancer, compare the difference between clinical and surgical staging of endometrial cancer and elucidate the advantages and feasibility of its surgical staging.

Methods: Fifty-six patients diagnosed pre-operatively as stage I endometrial cancer were reviewed for surgery for staging. They were assigned into laparoscopic group (n = 34) and laparotomic (open) group (n = 22).

[Clinical analysis of Metzenbaum scissors in transcervical incision of a complete septate uterus].

Objective: To evaluate the clinical efficacy of Metzenbaum scissors in the transcervical incision of a complete septate uterus.

Methods: Eight patients with a complete septate uterus were identified and recruited. The age range was 24 to 39 years old.

[Frozen-thawed embryo transfer (FET) versus fresh embryo transfer in clinical pregnancy rate during in vitro fertilization-embryo transfer].

Objective: To investigate whether frozen-thawed embryo transfer (FET) instead of fresh embryo transfer for the first embryo transfer (ET) in a controlled ovarian hyperstimulation (COH) cycle improves the in vitro fertilization-ET clinical pregnancy rate.

Methods: The investigators retrospectively analyzed the clinical data of 1341 IVF-ET cycles under COH, including 1169 fresh ET cycles (Group T1) and 172 FET cycles as the first embryo transfer in COH cycle (Group C1). Logistic regression was used to model the probability of clinical pregnancy rate based on age, ET method and other cycle-specific factors.

[Study of the best embryo transfer strategy in frozen-thawed embryo transfer cycle].

Objectives: To study the relationship of the number and quality of embryos transferred with clinical pregnancy rate and multiple pregnancy rate in frozen-thawed embryo transfer (FET) cycle.

Methods: Retrospective analysis of the clinical data of 995 FET cycles. Patients were stratified as age < 35 or > or = 35, Both groups' clinical pregnancy rate and multiple pregnancy rate were compared according to the number of embryos and high-quality embryos transferred.

Calcium-binding protein S100P is highly expressed during the implantation window in human endometrium.

Objective: To investigate S100P expression and localization in human endometrium throughout the menstrual cycle.

Design: Experimental study.

Setting: University hospital.

A frozen-thawed embryo transfer program improves the embryo utilization rate.

Background: Frozen-thawed embryo transfer (FET) is the most common way to prevent serious late ovarian hyperstimulation syndrome and increase the cumulative pregnancy rate. We evaluated the effectiveness of an FET program for improving the embryo implantation and clinical pregnancy rates, and ultimate embryo utilization rate in infertility treatment.

Methods: Patients undergoing in vitro fertilisation (IVF) cycles from January 2006 to June 2008 were enrolled, including 179 patients who had undergone the first FET cycle after controlled ovarian hyperstimulation (COH) in which all embryos were frozen (group C1) and 1306 patients who had COH with fresh embryo transfer (ET) (group T1).

Cervical cerclage before blastocyst transfer in patients having cryopreserved embryo transfer.

Objective: To present two successful pregnancy results following cervical cerclage before blastocyst transfer in patients who had cryopreserved ET and in whom cervical insufficiency was diagnosed.

Design: Case report.

Setting: Assisted reproduction unit in a university hospital.