[Construction and expression of recombinant adenovirus containing human catalase gene in vitro].

Objective: To construct the adenovirus vector containing recombinant human catalase (CAT) and to express the recombinant gene in vitro.

Methods: Total RNA was extracted from human leukocytes and full-length human CAT cDNA was obtained with RT-PCR method. The CAT gene was cloned into pcDNA3.