Multi-intervention integrated deworming strategy for sustained control of soil-transmitted helminths infections: a case study in Jiangsu Province, China.

Background: Soil-transmitted helminths (STH) infections still present a global health problem. Mass drug administration (MDA) is a widely applied strategy to reduce morbidity and mortality caused by STH. Yet, this approach has some shortcomings.

[Current status of chronic filariasis in Jiangsu Province].

Objective: To understand the current status of chronic filariasis patients in Jiangsu Province so as to provide basic data for following-up care for them.

Methods: The patients were followed up one by one according to history archives between June and July, 2018, and the clue investigation was also conducted. The base data of the patients was collected through a face-to-face questionnaire survey and analyzed.

[Analysis of effect of prevention and control technique training of human important parasitic diseases in Jiangsu Province in 2017].

Objective: To evaluate the effect of the prevention and control technique training of human important parasitic diseases for basic professional and technical personnel in Jiangsu Province in 2017.

Methods: A workshop including theory courses and practical operations was carried out for the basic professional and technical personnel. At the end of the workshop, the effects were evaluated through the theoretical and microscopic examinations.

[Epidemiological Survey on Intestinal Parasitic Infections in Lhasa City in 2014].

Objective: To investigate the prevalence of parasitic infections in Lhasa, Tibet.

Methods: The residents in Chengguan District, Dangxiong County, Dazi County and Qushui County were selected to participate in the study in 2014, using the stratified random sampling method. Infections with the soil-borne nematodes in feces were examined in triplicates using the Kato-Katz method.

[Investigation on soil - transmitted nematode infections in national surveillance sites in Jiangsu Province from 2006 to 2015].

Objective: To understand the status of soil-transmitted nematode infections in rural residents so as to provide the evidence for formulating the guidance for prevention and control of the diseases.

Methods: The national surveillance sites of soil-transmitted nematode infections were established in Shuyang County, Suqian City, Jiangsu Province from 2006 to 2015. At least 1 000 fecal samples of residents aged 3 years or above were collected in every autumn, and the intestinal helminth eggs were detected with the Kato-Katz technique and the eggs were detected by the cellophane tape method for children aged 3-12 years.

Alterations of physiology and gene expression due to long-term magnesium-deficiency differ between leaves and roots of Citrus reticulata.

Seedlings of Ponkan (Citrus reticulata) were irrigated with nutrient solution containing 0 (Mg-deficiency) or 1mM MgSO4 (control) every two day for 16 weeks. Thereafter, we examined magnesium (Mg)-deficiency-induced changes in leaf and root gas exchange, total soluble proteins and gene expression. Mg-deficiency lowered leaf CO2 assimilation, and increased leaf dark respiration.

[Epidemiological investigation of second intermediate hosts of Clonorchis sinensis in Pizhou and Xinyi cities of Jiangsu Province].

Objective: To understand the endemic situation of Clonorchis sinensis in its second intermediate hosts in Pizhou and Xinyi cities of Jiangsu Province, so as to provide the evidence for the further control and treatment.

Methods: Pseudorasbora parva and Abbottina rivularis were caught from the natural water body of Pizhou and Xinyi cities, and the tabletting microscopy method was applied to test the metacercaria of C. sinensis.

[Surveillance of echinococcosis in Liyang City from 2008 to 2012].

Objective: To investigate the Echinococcus infection of people and domestic animals in Liyang City, so as to provide the evidence for the establishment of control measures of echinococcosis.

Methods: The formerly confirmed cases and clue cases were investigated. The Echinococcus antibody in serum of children aged from 7 to 12 years old and adults in key towns and control towns was detected with ELISA, and the antigen in fecal samples of dogs in key towns was also detected.

[Effects of training of human intestinal parasitic diseases for basic health staff in Jiangsu Province].

Objective: To evaluate the effect of the training of human intestinal parasitic diseases for basic health staff.

Methods: A workshop including theory courses and practical operations was carried out. At the end of the workshop, the effects were evaluated through the examinations of theory and film-reading.

[Prediction of hookworm incidence with time-series model in Jiangsu Province].

Objective: To explore the feasibility of autoregressive integrated moving average (ARIMA) to predict the infection rates of hookworm in Jiangsu Province.

Methods: From 1990 to 2006, the infection rates of hookworm were used for a training data set. As to obtain a stationary data set, the training data set was second-order differenced using the version SAS 9.

[Surveillance of soil-transmitted nematodiasis in northern Jiangsu Province from 2006 to 2010].

Objective: To evaluate the control effect on soil-transmitted nematodiasis in surveillance sites of northern Jiangsu Province.

Methods: According to "The Monitoring Program on Soil-transmitted Nematodiasis in Jiangsu Province", the eggs of soil-transmitted nematodes were detected by the Kato-Katz technique and cellophane anal swab technique, and the infection rates were predicted by the horizontal average speed development method in the surveillance sites from 2006 to 2010.

Results: The overall infection rate of soil-transmitted nematodes declined to 3.

[Cloning, expression and evaluation on effect in serological diagnosis of cysteine protease of Clonorchis sinensis].

Objective: To clone and express the cysteine protease of Clonorchis sinensis and evaluate its effect on immunodiagnosis of human clonorchiasis.

Methods: Based on a cysteine protease gene fragment of C. sinensis (CS-CP, GenBank accession: AF093242), a pair of primers were designed and amplified from total cDNA of C.

[Preliminary study on detection of Echinococcus granulosus DNA by loop-mediated isothermal amplification].

Objective: To evaluate the sensitivity of loop-mediated isothermal amplification (LAMP) on the detection of Echinococcus granulosus.

Methods: The DNAs were extracted from Echinococcus granulosus eggs and adults. According to Echinococcus mitochondrial 12S rRNA sequences and the mechanism of LAMP, 4 Echinococcus specific primers were designed and used for LAMP assay, and Bubble taenia and the blank were used as the negative control for evaluation of the specificity.

[Performance evaluation of rural latrine renovation in key schistosomiasis control areas, Jiangsu Province].

Objective: To evaluate the hygienic, environmental, social and economic benefits achieved by rural latrine renovation in key schistosomiasis control areas, Jiangsu Province.

Methods: Eights villages (evaluation villages) that finished and the three villages (control villages) that did not finish the latrine renovation work were sampled and investigated through field visits, questionnaire, referring to information and so on, to collect relevant data, and human waste samples were detected for laboratory indicators in hygiene and environment, in key schistosomiasis control areas in three counties (cities, districts), Jiangsu Province.

Results: A total of 11 villages and 220 households were investigated.

[Purification of Clonorchis sinensis native protein and their application in immunodiagnosis of clonorchiasis].

Lots of purified antigens have been obtained from crude antigens of Clonorchis sinensis. Some of them have applied to immunodiagnosis of clonorchiasis in laboratory. Here, we review purification methods, diagnostic efficacy and application prospect of purified antigens.

[Process for lymphatic filariasis elimination in Jiangsu Province, China].

Objective: To review the process of lymphatic filariasis elimination in Jiangsu Province, and summarize the control experience.

Methods: The data of epidemic status, control and surveillance of lymphatic filariasis were collected and analyzed in Jiangsu Province from 1950s to 1990s.

Results: Lymphatic filariasis was endemic in 71 counties (cities) among all 75 counties (cities) in Jiangsu Province.

[Epidemic situation and control of soil-transmitted nematodiasis in Jiangsu Province].

Objective: To analyze the prevalence changes and the control measures of soil-transmitted nematodiases in Jiangsu Province in the recent 20 years and evaluate the control effect, so as to explore the control strategies and measures appropriate to current epidemic characteristics.

Methods: The data on surveillance, control measures and control effect of soil-transmitted nematodiases in Jiangsu Province from 1990 to 2009 were collected and analyzed.

Results: Different control programs were taken in different control stages in Jiangsu Province.

[Malaria epidemic situation in Jiangsu Province in 2006].

In 2006, there were 767 reported malaria cases in Jiangsu Province with an incidence of 1.07 per thousand and increased by 16.57% in comparison to the previous year.