Changes in Gel Characteristics, Rheological Properties, and Water Migration of PSE Meat Myofibrillar Proteins with Different Amounts of Sodium Bicarbonate.

The changes in the gel and rheological properties and water-holding capacity of PSE meat myofibrillar proteins with different amounts of sodium bicarbonate (SC, 0−0.6/100 g) were studied. Compared to the PSE meat myofibrillar proteins with 0/100 g SC, the texture properties and cooking yield significantly increased (p < 0.

Effect of sodium bicarbonate on techno-functional and rheological properties of pale, soft, and exudative (PSE) meat batters.

In the study, changes in salt-soluble protein (SSP) content, gel properties, rheological characteristic, and microstructure attributes of pale, soft, and exudative (PSE) pork batters with different concentrations of added sodium bicarbonate (0-0.6%) were investigated. The pH, b value, SSP content, cooking yield, texture properties, emulsion stability, and G' values at 72 °C significantly increased with the increase in sodium bicarbonate, but the texture properties and G' values of the samples with 0.

[Determination of Haloacetic Acids, Disinfection Byproducts, in Tap Water with Reversed-Phase Ultra-Performance Liquid Chromatography-High Resolution Mass Spectrometry].

Objective: To establish a method for quantitative analysis of haloacetic acids (HAAs), disinfection byproducts, in tap water with reversed-phase ultra-performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry.

Methods: Tap water samples were collected and 0.70 g/L ascorbic acid was added to eliminate residual chlorine.

[The Screening Approaches for 34 Common Drugs and Metabolites in Biological Samples by Liquid Chromatography Orbital Trap Mass Spectrometry].

Objective: To establish a high-performance liquid chromatography orbital trap mass spectrometry (HPLC-Obitrap MS) method for screening 34 common drugs and metabolites in biological samples.

Methods: The target analytes in urine and blood samples were extracted with ethyl acetate, concentrated by nitrogen blowing and redissolved. The hair samples were washed with water and acetone, dried and cut into bits of about 1 mm, and then crushed in a freezing grinder.

[Study on the Analytical Method of Solid Phase Extraction-Ultra Performance Liquid Chromatography Tandem Quadrupole Linear Ion Trap Mass Spectrometry for 12 Perfluorinated Compounds in Human Urine].

Objective: To establish a method for simultaneous determination of 12 kinds of perfluorinated compounds (PFCs) in human urine based on ultra performance liquid chromatography tandem quadrupole linear ion trap mass spectrometry (UPLC-QTtrap-MS).

Methods: After pH adjustment with 2% formic acid, the urine samples were loaded on a WAX solid phase extraction (SPE) cartridge for extraction, purification and concentration. The eluates were collected, concentrated to dryness under nitrogen, and reconstituted with 10 mmol/L ammonium acetate aqueous solution-methanol ( ∶ = 70∶30) before injection.

Takeaway food in Chengdu, Sichuan province, China: Composition and nutritional value.

Background And Objectives: The popularity of takeaway has caused health problems. To analyse the basic nutrients and composition of popular takeaway meals in Chengdu, China.

Methods And Study Design: We randomly collected 105 takeaway meals from takeaway platforms.

[Determination of Aromatic Compounds Metabolites in Human Urine by Solid Phase Extraction-liquid Chromatography-Tandem Mass Spectrometry].

Objective: To establish the method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with solid phase extraction (SPE) for simultaneous determination of the biological metabolites of aromatic compounds, including N-acetyl-S-phenyl-L-cysteine (SPMA), N-acetyl-S-benzyl-cysteine (SBMA), p-nitrophenol (PNP), methylhippuric acids (MHA), p-Aminophenol (PAP), mandelic acid (MA), phenylglyoxylic acid (PGA) and 1-hydroxypyrene (1-OHP) in urine.

Methods: After adding 20 μL of β-glucuronidase and 1 mL ammonium acetate buffer solution in 1 mL of urine, the sample was digested in a 37 ℃ incubator for 20 h. After digestion, the enzymatic hydrolysate was purified by PRIME HLB solid phase extraction column.

[Detecting Nicotine and Cortinine in Hair by Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry].

Objective: To develop a method for detecting nicotine and cotinine in hair by hydrophilic interaction chromatography tandem mass spectrometry.

Methods: Hair samples were hydrolyzed in sodium hydroxide solution before extraction with dichloromethane. The samples were blown to dry with nitrogen and dissolved with mobile phase.

[Detection of 11 Organophosphate Pesticides and Atrazine in Water by Dispersive Liquid-Liquid Microextraction Combined with Gas Chromatography Mass Spectrometry].

Objective: To develop a method for detecting 11 organophosphate pesticides and atrazine in water samples by dispersive liquidliquid microextraction combined with gas chromatographymass spectrometry (DLLMEGC/MS) .

Methods: DLLME and GCMS parameters were optimized for efficient extraction of chemicals. The proposed method was used for detecting organophosphate pesticides in tap water and river water samples,with 200 μL of dimethylbenzene as extractant and 800 μL of methanol as dispersant.

Bone marrow mesenchymal stem cells repair cadmium-induced rat testis injury by inhibiting mitochondrial apoptosis.

Cadmium is a highly toxic metal with widespread exposure to people that can cause tissue injuries that lack effective treatment. The aim of this project was to uncover whether bone marrow mesenchymal stem cells (BMSCs) can repair cadmium-induced rat testis injury and to explore the role of mitochondrial apoptosis in this process. To this end, 21 adult male Wistar rats were randomly divided into control, model and therapy groups, 7 each, and were administered 0, 0.

[Determination of Nicotine and Cotinine in Urine by Dispersive Liquid-Liquid Microextraction Combined with Gas Chromatography-Mass Spectrometry].

Objective: To develop a method for the determination of nicotine and cotinine in urine samples by dispersive liquid-liquid microextraction (DLLME) combined with gas chromatography-mass spectrometry (DLLME-GC/MS).

Methods: The experimental conditions for GC-MS and DLLME were investigated in detail. DLLME was performed with the following procedure: 5 mL of urine sample was adjusted to pH9.

[Effectiveness of CLAT Protocol for Treating Patients with Refractory Acute Myeloid Leukemia].

Objective: To explore the clinical efficacy and toxicity of CLAT protocol (cladribine, cytarabine and topotecan) for treating patients with refractory acute myeloid leukemia (R-AML).

Methods: A total of 18 patients with R-AML (median age 37 years, range 18 to 58 years; male n = 16, female n = 2) were treated with CLAT protocol, which consisted of cladribine 5 mg/m(2)/d, i.v.

[Detecting Thallium in Water Samples using Dispersive Liquid Phase Microextraction-Graphite Furnace Atomic Absorption Spectroscopy].

Objective: To develope a method of solvent demulsification dispersive liquid phase microextraction (SD-DLPME) based on ion association reaction coupled with graphite furnace atomic absorption spectroscopy (GFAAS) for detecting thallium in water samples. Methods Thallium ion in water samples was oxidized to Tl(III) with bromine water, which reacted with Cl- to form TlCl4-. The ionic associated compound with trioctylamine was obtained and extracted.

Association of increased circulating catecholamine and glucocorticoid levels with risk of psychological problems in oral neoplasm patients.

Background: Noradrenergic pathways and glucocorticoid-mediated signal pathways have been implicated in the growth and progression of oral cancer. Patients with oral neoplasms can have high psychological distress levels, but the effects of stress-related hormones on oral neoplasm growth are unknown.

Methods: We have investigated the relationships between pre-surgical measurements of psychological problems with Symptom Checklist-90-revised Inventory (SCL90-R), tumor histology, circulating blood catecholamine and glucocorticoid levels among 75 oral neoplasm patients, including 40 oral cancer patients and 35 benign oral tumor patients.

[Simultaneous determination of adrenaline, noradrenaline, cortisone and cortisol in plasma with HPLC/MS/MS].

Objective: To develop a method for simultaneous determination of adrenaline, noradrenaline, cortisone and cortisol in plasma using HPLC/MS/MS.

Methods: Sample proteins were precipitated with acetonitrile and the sample solution was injected into HPLC/MS/MS after centrifugation at 15,000 r/min for 5 min. Electrospray ionization (ESI) and the positive ion detection were applied with a multiple reaction monitoring (MRM) mode for quantitative analyses.

[Assessment of mental health status in oral squamous cell carcinoma patients and its correlation with catecholamines level].

Purpose: To investigate the relationship between mental health status and catecholamines level in oral squamous cell carcinoma patients.

Methods: Forty patients with oral squamous carcinoma (OSCC) who were diagnosed in West China School of Stomatology between Dec. 2011 and Aug.

[Determination of canthaxanthin and astaxanthin in egg yolks by reversed phase high performance liquid chromatography with diode array detection].

Objective: A method using reversed phase high performance liquid chromatography (RP-HPLC) coupled with diode array detector (DAD) was developed for the simultaneous determination of canthaxanthin and astaxanthin in egg yolks.

Methods: Samples were extracted with acetonitrile in ultrasonic bath for 20 minutes and then purified by freezing-lipid filtration and solid phase extraction (SPE). After being vaporized to dryness by nitrogen blowing and made up to volume with methanol, the extract solution was chromatographically separated in C18 column with a unitary mobile phase consisting of acetonitrile.

[Determination of histatins 5 in salivary by reversed-phase high performance liquid chromatography method with ultraviolet detection].

Objective: The determination method of histatins 5 in human saliva with reversed-phase high performance liquid chromatography (HPLC) was developed.

Methods: Salivary samples were collected and diluted with phosphate buffer (pH 2.5).

[Rapid determination of ethanol in blood by capillary-gas chromatography].

Objective: To develop a method for the rapid determination of ethanol in blood with capillary-GC.

Methods: 0.50 mL of whole blood sample was taken and added with 1.

[Determination of adenosine in food by high performance anion exchange chromatography].

Objective: To establish a high performance anion chromatography method for the determination of adenosine in food.

Methods: Adenosine in the food sample was extracted with Acetonitrile-water, and separated with Amino PAC PA10 Amino acid-separation column, with 0.25 mol/L NaOH as the mobile phase.

[Determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food by high performance anion exchange chromatography].

Objective: To develop a method for analysis of eight kinds of monosaccharides using high performance anion exchange chromatography.

Methods: Amino PAC PA-10 (2 x 250 mm) column with a pulse amperometric detector with an Au working electrode and an Ag/AgCl reference electrode was applied to determine the monosaccharides.

Results: The linear range was 0.

[Determination of organic acids in dental plaque with high performance liquid chromatography].

Objective: To establish determination method of formic acid, lactic acid, acetic acid and succinic acid in dental plaque with high performance liquid chromatography (HPLC).

Methods: After the samples were centrifuged, 2 microL supernatant was transferred to a 1 mL centrifuge tube and diluted in water, then was determined with HPLC. The mixture of phosphate buffer and methanol (97:3) as mobile phase throughout the experiment.

[Detecting VA, VD3, VE and beta-carotene with HPLC in human serums].

Objective: To develop a method of detecting retinol (vitamin A), vitamin D3, alpha-tocopherol (vitamin E) and beta-carotene in human serums with HPLC.

Methods: Proteins were precipitated with anhydrous ethanol. Fat-solutable vitamins in human serums were extracted with aether/petroleum ether mixture and determined with HPLC.