[Inhibition of HPV16 E6 oncogene in cervical cancer by RNA interference].

Objective: The efficiency of HPV16 E6 gene silenced by RNA interference in vitro and in vivo was assessed.

Methods: The specific siRNA of HPV16 E6 was designed and transfected into CaSki cells by liposome. Cell apoptotic rates and the changes in HPV16 E6 mRNA and protein before and after transfection were measured.

[Chemosensitivity testing of topotecan as second-line agent in chemotherapy of ovarian cancer].

Background & Objective: The therapeutic principle of ovarian cancer was cytoreductive surgery assisting with chemotherapy. At present, cisplatin-based chemotherapy is the primary way for treatment of ovarian cancer. But most patients with advanced ovarian cancer were easy to recurrent and then become resistance to cisplatin.

[Tissue microarray and in situ hybridization study on hypoxia inducible factor 1alpha mRNA expression in epithelial ovarian tumor].

Objective: To study the expression and significance of hypoxia inducible factor 1alpha (HIF-1alpha) in epithelial ovarian tumors.

Methods: The expression of HIF-1alpha mRNA in 295 patients with epithelial ovarian tumor was analyzed retrospectively by high-throughput tissue microarray and in situ hybridization, which was compared with 13 normal ovarian tissue samples.

Results: The expression rates of HIF-1alpha mRNA were 0, 13.

[Expression of lung resistant-related protein in ovarian cancer and its clinical significance].

Background & Objective: It was reported that there was negative correlation between the over-expression of lung resistant-related protein (LRP) and the sensitivity of cisplatin and cyclophosphamide. Also it was reported that the over-expression of LRP could predict the chemosensitivity and prognosis of ovarian cancer, and it was an independent index of remission and exist period. This study was designed to investigate the function of LRP in cisplatin resistance and the relationship with prognosis of ovarian cancer by combining with the chemosensitivity test.