Restore a 9 mm diameter osteochondral defect with gene enhanced tissue engineering followed mosaicplasty in a goat model.

Objective: The aim of this study was to evaluate the efficacy of gene enhanced tissue engineering followed mosaicplasty in a goat model.

Methods: An acute cylindrical defect 9 mm in diameter was created in the weight bearing area of the medial femoral condyle in a goat model. Thirty-six medial femoral condyles were divided into 6 groups using different proportion of gene enhanced tissue engineering and mosaicplasty to restore the defects.

Incongruity affecting osteochondral repair by mosaicplasty technique in an animal model.

Objective: To compare the influence of the different osteochondral incongruity grades on the osteochondral repair by the mosaicplasty technique in a goat model.

Methods: The acute defects were created in the weight-bearing area of the medial femoral condyle of the goats. Mosaicplasty was done to repair the defects.

[Research on the repair of acute large osteochondral defects with mosaicplasty associated with genes-enhanced tissue engineering in different proportion].

Objective: To investigate the optimum proportion of Mosaicplasty and genes-enhanced tissue engineering for the repair of acute osteochondral defects.

Methods: Western blot test was conducted to detect the expression of hTGF-beta1, Col II and Aggrecan in 3 groups, including hTGF-beta1, transduction group, Adv-betagal transduction group and control group without transduction. Eighteen 6-month-old Shanghai male goats (weight: 22 to 25 kg) were used.

[Influence of the unevenness of articular surface on the osteochondral repair].

Objective: To investigate whether the unevenness of articular surface would affect the osteochondral repair.

Methods: Eight Shanghai Chongming 6-months-old masculine goats with a mean weight of 25 kg were used in this study. Different unevenness, which were 0.

Mosaicplasty associated with gene enhanced tissue engineering for the treatment of acute osteochondral defects in a goat model.

Objective: To compare single mosaicplasty, mosaicplasty associated with gene enhanced tissue engineering and mosaicplasty associated with the gels of non-gene transduced bone mesenchymal stem cells (BMSCs) in alginate for the treatment of acute osteochondral defects in a goat model.

Methods: The principle and methods of tissue engineering were used. BMSCs were separated and amplified in vitro, and human transforming growth factor-beta1 (hTGF-beta1) gene was transduced to the cells.

[The study of bipolar radiofrequency chondroplasty to cartilage injure of goats].

Objective: To study the evaluation of bipolar radiofrequency (RFE) chondroplasty to cartilage injure.

Methods: Sixteen goats underwent resection of anterior cruciate and medial meniscus to create cartilage injured model. Bipolar frequency energy chondroplasty were performed on injured articular cartilage 3 months after operation.

[Experimental research on human insulin-like growth factor I gene transfect the cultured bone marrow mesenchymal stem cells].

Objective: To investigate the effectiveness of human insulin-like growth factor I (hIGF-I) gene transferred into the cultured goat bone marrow mesenchymal stem cells with liposome, and find a new method of cell-mediated gene therapy.

Methods: Bone marrow was extracted from adult goats and cultured in vitro by monolayer. Then the recombinant eukaryotic expression plasmid pIRES2-EGFP-hIGF was transfected into cells by FuGene 6.

[Experimental research on spine fusion induced by tissue engineered bone].

Objective: To construct a reasonable substitute for the autograft bone in vitro and transplant it back into the rabbit models to induce the spine fusion.

Methods: The bone marrow stem cell from the seven New Zealand rabbits were cultured. Recombinant human bone morphogenetic protein-4 (rhBMP-4) that has been proved to be bioactive was obtained by the way of genetic engineering.