[Transurethral enucleation plus pneumo-cystostomy rotary cut for large benign prostatic hyperplasia].

Objective: To investigate the feasibility, effectiveness and practicability of transurethral enucleation plus pneumocystostomy rotary cut (TUE + PCRC) for large benign prostatic hyperplasia (BPH).

Methods: We performed TUE + PCRC for 26 BPH patients aged 62 - 85 years with the prostate volume of 80 - 165 ml. We conducted transurethral enucleation of the hyperplastic prostate glands and pushed them into the bladder, followed by bladder puncture for pneumo-cystostomy rotary cut.

[Expressions of cadherin molecules CDH18 and PCDH17 in human azoospermic testes].

Objective: To investigate the expressions of cadherin molecules CDH18 and PCDH17 in normal and azoospermic human testes and their significance.

Methods: We studied the routine pathological slices of normal and non-obstructive azoospermic human testis tissues for changes in the tight junction of Sertoli-germ cells, and identified the differential gene expression profiles of the normal and azoospermic testis tissues using cDNA microarrays containing multiple cadherin molecules. The results were confirmed by Western blot.

[X-ray guided internal urethroplasty with PlasmaKinetic electrodes for urethratresia].

Objective: To evaluate endourethral surgery for urethratresia under the X-ray guide.

Methods: We performed transurethral urethroplasty for 11 patients with urethratresia using the PlasmaKinetic electrodes under the guidance of C arm xanthippe.

Results: In the 11 cases, operations were all successful, 9 achieved smooth urination and 2 needed regular urethral dilation.

[Expression of COX10 in human non-obstructive azoospermia testes].

Objective: To evaluate the expression of COX10 mRNA in the testes of non-obstructive azoospermia patients and normal men.

Methods: A cDNA microarray containing COX10 and some other genes as RBM and EIF1AY was used to identify the differential gene expression profiles in the normal and azoospermic testes. The cDNA probes were prepared by labeling mRNA from azoospermic and normal testis tissues with Cy5-dUTP and Cy3-dUTP respectively through reverse transcription.

[Expression of cell cycle molecules in human azoospermic testes].

Aim: To evaluate the expression and significance of cell cycle molecules in human normal and azoospermia testes.

Methods: A cDNA microarray containing cDNA of some cell cycle molecules was used to identify the differential gene expression profiles between normal and azoospermic testes. cDNA probes were prepared by labeling mRNA from normal and testis tissues with Cy5-dUTP and Cy3-dUTP, respectively, through reverse transcription.

[Antisense oligonucleotide targeting survivin induces apoptosis of renal clear-cell carcinoma cells and enhances their sensitivity to epirubicin in vitro].

Objective: To investigate the effect of antisense oligonucleotide (ASODN) targeting survivin on the apoptosis and proliferation of renal cancer cell line 786-O and enhancement of its sensitivity to epirubicin.

Methods: ASODN targeting survivin was designed and constructed. Cultured cells were divided into 6 groups: control group, liposome group, sense oligonucleotide (SODN) group, 600 nmol/L ASODN group, and 600 nmol/L ASODN combined with epirubicin group.

Expression and significance of Rap1A in testes of azoospermic subjects.

Aim: To evaluate the Rap1A mRNA expression and its significance in the testes of normal and azoospermic subjects.

Methods: A cDNA microarray that contained Rap1A and some other genes such as RBM, EIF1AY was used to identify the differential gene expression profiles between the normal and azoospermic testes. cDNA probes were prepared by labeling mRNA from azoospermic and normal testicular tissues through reverse transcription with Cy5-dUTP and Cy3-dUTP, respectively.

[Protective effect of nitric oxide synthase inhibitor (L-NAME) on germ cell apoptosis in experimentally cryptorchid rats].

Objective: To explore the protective effect of nitric oxide synthase inhibitor (L-NAME) on the germ cell apoptosis in the rat cryptorchid.

Methods: Immature rats (22 day-old Sprague Dawley) were subjected to unilateral cryptorchid. Thirty rats were divided into three groups: sham operation group (testes still in the scrotum after operation); operation group; operation + L-NAME group(given L-NAME 10 mg/kg after operation, dip).

[A study of aspermia-related genes by genchips and analysis of the RAP1A gene].

Objective: To study the differential gene expression profiles between the normal and aspermia human testes by genechips.

Methods: Probes were prepared from mRNA extracted from both normal and aspermia testes and employed on Biostar H-40s genechips to detect the differential gene expression profiles. A distinctly up-regulated gene RAP1A was analyzed by bibliogrphic retrieval.