Virome Analysis of Reveals Infection by Eleven Viruses, including Two Potentially New Species.

is a herbaceous herb indigenous to China that has been cultivated for traditional medicine for centuries. Virus-like symptoms of plants were observed on leaves in some plantations in Zhanyi and Wuding Counties, Yunnan Province, southwest China. High-throughput sequencing (HTS) was performed on 28 symptomatic plants, and the results revealed infection with 11 viruses, including 2 novel viruses and 9 previously described viruses: Aconitum amalgavirus 1 (AcoAV-1), aconite virus A (AcVA), cucumber mosaic virus (CMV), currant latent virus (CuLV), apple stem grooving virus (ASGV), chilli veinal mottle virus (ChiVMV), tomato spotted wilt orthotospovirus (TSWV), tobacco vein distorting virus (TVDV), and potato leafroll virus (PLRV).

Protective effect and mechanism of Polygonatum kingianum against hypoxia-induced injury.

Background: Hypoxia is an essential cause of fatigue and aging, and is associated with the occurrence and development of many diseases. (PK) is a deficiency-nourishing Chinese herbal medicine utilized as both medicine and food, and it has long been used to ameliorate human conditions associated with fatigue and aging over 2000 years in China. PK is an important genuine-medicinal-materials cultivated in Yunnan, China, and is used by the Bai, Wa, and Zhuang nationalities as a traditional medicine for enhancing immunity, anti-fatigue, and anti-aging, while the preventive effect of PK on hypoxia-induced injury and the underlying mechanism are indefinite.

Cathepsin C promotes colorectal cancer metastasis by regulating immune escape through upregulating CSF1.

Since metastasis remains the primary reason for colorectal cancer (CRC) associated death, a better understanding of the molecular mechanism underlying CRC metastasis is urgently needed. Here, we elucidated the role of Cathepsin C (CTSC) in promoting CRC metastasis. The expression of CTSC was detected by real-time PCR and immunohistochemistry in the human CRC cohort.

High-throughput sequencing reveals the presence of novel and known viruses in diseased .

spp. are important medicinal plant and main raw material for many Chinese patent medicines, but viral diseases have became serious problems in cultivation of this group of important medicinal plants in China. In this study, eight viruses were identified in the diseased plants of by high-throughput sequencing (HTS) and RT-PCR.

Extracts of (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways.

(Spreng.) M. A.

Tang-Ping-San Decoction Remodel Intestinal Flora and Barrier to Ameliorate Type 2 Diabetes Mellitus in Rodent Model.

Purpose: Type 2 diabetes mellitus (T2DM) is a complex genetic disease associated with genetic and environmental factors. Previous studies have shown that changes in the gut microbiota may affect the development of host metabolic diseases and promote the progression of T2DM. Tang-ping-san (TPS) decoction can effectively treat T2DM.

Complete genome sequence of Aconitum amalgavirus 1, a distinct member of the genus Amalgavirus.

A novel virus named Aconitum amalgavirus 1 (AcoAV-1) was identified in Chinese aconite (Aconitum carmichaelii) plants. The complete genome of AcoAV-1 is 3,370 nucleotides long, containing two partially overlapping open reading frames encoding a putative coat protein and a RNA-dependent RNA polymerase, respectively. Its fusion protein shares 34.

Genomic characterization of a new enamovirus infecting common bean.

A novel enamovirus was identified in common bean plants with disease symptoms. Its genome of 5,781 nucleotides (nt) contains five open reading frames. This virus and other members of the genus Enamovirus share 50.

Identification of two novel poleroviruses and the occurrence of Tobacco bushy top disease causal agents in natural plants.

Tobacco bushy top disease (TBTD) is a devastating tobacco disease in the southwestern region of China. TBTD in the Yunnan Province is often caused by co-infections of several plant viruses: tobacco bushy top virus (TBTV), tobacco vein distorting virus (TVDV), tobacco bushy top virus satellite RNA (TBTVsatRNA) and tobacco vein distorting virus-associated RNA (TVDVaRNA). Through this study, two new poleroviruses were identified in two TBTD symptomatic tobacco plants and these two novel viruses are tentatively named as tobacco polerovirus 1 (TPV1) and tobacco polerovirus 2 (TPV2), respectively.

The association between orthostatic blood pressure changes and subclinical target organ damage in subjects over 60 years old.

Background: Although recent studies have indicated that both orthostatic hypotension and orthostatic hypertension independently predict cardiovascular events, the underlying mechanisms are still controversial. The aim of the study was to investigate the relationships between orthostatic changes and organ damage in subjects over 60 years old.

Methods: This is a prospective observational cohort study.

Author Correction: Regional variation limits applications of healthy gut microbiome reference ranges and disease models.

In the version of this article originally published, in the sentence "Applying the same approach to obesity (Fig. 2b), MetS (Fig. 2c) and fatty liver (Fig.

Linking gut microbiota, metabolic syndrome and economic status based on a population-level analysis.

Background: The metabolic syndrome (MetS) epidemic is associated with economic development, lifestyle transition and dysbiosis of gut microbiota, but these associations are rarely studied at the population scale. Here, we utilised the Guangdong Gut Microbiome Project (GGMP), the largest Eastern population-based gut microbiome dataset covering individuals with different economic statuses, to investigate the relationships between the gut microbiome and host physiology, diet, geography, physical activity and socioeconomic status.

Results: At the population level, 529 OTUs were significantly associated with MetS.

Regional variation limits applications of healthy gut microbiome reference ranges and disease models.

Dysbiosis, departure of the gut microbiome from a healthy state, has been suggested to be a powerful biomarker of disease incidence and progression. Diagnostic applications have been proposed for inflammatory bowel disease diagnosis and prognosis, colorectal cancer prescreening and therapeutic choices in melanoma. Noninvasive sampling could facilitate large-scale public health applications, including early diagnosis and risk assessment in metabolic and cardiovascular diseases.

Bioactive norditerpenoids from Cephalotaxus fortunei var. alpina and C. lanceolata.

Twenty-eight naturally occurring Cephalotaxus tropone analogues, including 19 previously undescribed ones, were identified from Cephalotaxus fortunei Hook. var. alpina H.

The sex ratio of singleton and twin delivery offspring in assisted reproductive technology in China.

In order to evaluate the impact of assisted reproductive technology (ART) procedure and individual factors on the sex ratio of singletons and twins at birth after in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI) treatment in China. We conducted a retrospective cohort study including patients undergoing their first cycle of IVF or ICSI with autologous oocytes from 2001 to 2015. A total of 7410 babies were born from 5832 women with 7410 live birth.

Abietane diterpenoids from Cephalotaxus lanceolata.

A new abietane diterpenoid, 12-O-methyl-20-deoxocarnosol-3-one (1), and eight known abietane diterpenoids including 13-abietadien-12-one (2), 5,6-dehydrosugiol (3), sugiol (4), torreyayunnin (5), taxusabietane A (6), hinokiol (7), 3-acetoxyabieta-8,11,13-trien-12-ol (8), and martiusane (9) were obtained from leaves and twigs of Cephalotaxus lanceolata. The structures of isolated compounds (1-9) were determined based on analysis of their spectroscopic data and comparison with those reported in the literature. Compounds 3, 8 and 9 were first isolated from the plants of Cephalotaxus genus.

Associations of prenatal exposure to phenols with birth outcomes.

Many phenols are known to mimic or antagonize hormonal activities and may adversely affect fetal growth. A study of 567 pregnant women was conducted to investigate the relationship between prenatal phenol exposure and birth outcomes, including birth weight, length, and gestational age. We measured the concentrations of bisphenol A, benzophenone-3, 4-n-octylphenol and 4-n-nonylphenol in maternal urine and examine their association with birth outcomes.

A new on-line concentration method of cationic molecules in capillary electrophoresis by a hyphenated micelle to solvent stacking coupling with large amount sample electrokinetic stacking injection.

In this paper, we established a new on-line method using micelle to solvent stacking (MSS) technique combining with large amount sample electrokinetic stacking injection (LASEKSI) for the analysis of cationic molecules. In this MSS-LASEKSI, by modulating the integral EOF across the capillary, a equilibrium state was formed and can be maintained for a long time, leading to the continuous stacking of the analytes on the basis of MSS. Thereby, an extremely large amount sample was permitted to be injected into the capillary and then an improved enrichment fold can be achieved comparing with the each case.

Graphene oxide-Fe3O4 magnetic nanocomposites with peroxidase-like activity for colorimetric detection of glucose.

In this report, graphene oxide-Fe(3)O(4) magnetic nanocomposites were demonstrated to possess intrinsic peroxidase-like activity and enhanced affinity toward H(2)O(2). The nanocomposites retain their magnetic properties and can be effectively separated by a magnet. Significantly, they were proved to be novel peroxidase mimetics which could quickly catalyze oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H(2)O(2), producing a blue-colored solution.

Phytoavailability of heavy metals in tidal flat soils after fresh water leaching.

The phytoavailability of Cd, Pb, Cr, Zn, Cu, and Ni, retained in tidal flat soil after fresh water leaching during reclamation was investigated. Two salt-tolerant varieties of tomato (Lycopersicon esculentum) and edible amaranth (Amaranthus mangostanus L.) were planted in soils having eight different desalination levels (from 6.