Stevioside induced cytotoxicity in colon cancer cells via reactive oxygen species and mitogen-activated protein kinase signaling pathways-mediated apoptosis.

The role of mitogen-activated protein kinase (MAPK) signaling pathways in cell growth and differentiation has been well established. The present study aimed to investigate the anti-proliferative effect of stevioside on human colon cancer HT-29 cells. Additionally, the effect of stevioside on cell cycle arrest and MAPK signaling pathways in HT-29 cells was explored.

[Vertical Distribution Characteristics and Analysis in Sediments of Xidahai Lake].

The organic matter (OM), total nitrogen (TN), total phosphorus (TP), the morphological changes of phosphorus and the particle size in columnar sediment core of Xidahai Lake were analyzed, to discuss the vertical distribution characteristics and influencing factors. The results showed that the contents of OM, TN and TP were 0. 633% -2.

Fast in situ generated ɛ-polylysine-poly (ethylene glycol) hydrogels as tissue adhesives and hemostatic materials using an enzyme-catalyzed method.

In this study, novel bio-inspired in situ hydrogels as tissue adhesives and hemostatic materials were designed and prepared based on ɛ-polylysine-grafted poly(ethylene glycol) and tyramine via enzymatic cross-linking. The enzymatic cross-linked method enabled fast gelation within seconds, which facilitated its therapeutic applications. By changing the cross-linking conditions, the storage modulus of the hydrogels could be tunable and the mechanical strength influenced the tissue adhesiveness of the hydrogels.

Gene cloning, expression, and characterization of an exo-inulinase from Paenibacillus polymyxa ZJ-9.

An inulinase-producing strain, Paenibacillus polymyxa ZJ-9, was isolated from natural sources to produce R,R-2,3-butanediol via one-step fermentation of raw inulin extracted from Jerusalem artichoke tubers. The inulinase gene from P. polymyxa ZJ-9 was cloned and overexpressed in Escherichia coli BL21 (DE3), and the purified recombinant inulinase was estimated to be approximately 56 kDa by both sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography.

[Evolvement and pollution of heavy metals in core sediments from Yamenqi Lake in Lianhuan Lake, China].

One sediment core was obtained from the center of Yamenqi Lake at the Songnen Plain in eastern China in August 2010 using a gravity corer. The sediment samples were digested using HNO3-HClO4-HF. Concentrations of the metals Cr, Cu, Mn, Ni, Fe, Pb, Zn, Cd, Al, Ba, Ca, K, Li, Mg, Na and Sr were determined using inductively coupled plasma-mass spectrometry (ICP-MS).

[Ichthyofauna and its community diversity in volcanic barrier lakes of Northeast China].

Based on the investigations of fish resources in Jingpo Lake and Wudalianchi Lakes in 2008-2011 and the historical data, this paper analyzed the characteristics of ichthyofauna and its community diversity in volcanic barrier lakes of Northeast China. The ichthyofauna in the volcanic barrier lakes of Northeast China was consisted of 64 native species, belonging to 47 genera, 16 families, and 9 orders, among which, one species was the second class National protected wild animal, four species were Chinese endemic species, and five species were Chinese vulnerable species. In the 64 recorded species, there were 44 species of Cypriniformes order and 37 species of Cyprinidae family dominated, respectively.

An oxidoreduction potential shift control strategy for high purity propionic acid production by Propionibacterium freudenreichii CCTCC M207015 with glycerol as sole carbon source.

The effects of oxidoreduction potential (ORP) regulation on the process of propionic acid production by Propionibacterium freudenreichii CCTCC M207015 have been investigated. Potassium ferricyanide and sodium borohydride were determined as ORP control agents through serum bottle experiment. In batch fermentation, cell growth, propionic acid and by-products distribution were changed with ORP levels in the range of 0-160 mV.

A 2,3-butanediol dehydrogenase from Paenibacillus polymyxa ZJ-9 for mainly producing R,R-2,3-butanediol: purification, characterization and cloning.

A 2,3-butanediol dehydrogenase (BDH) from Paenibacillus polymyxa ZJ-9 was purified to homogeneity via fractional ammonium sulfate precipitation, followed by two steps of anion-exchange chromatography using DEAE-Sepharose and Source 15Q, obtaining a 35-fold increase in specific activity and 34.9% yield. The molecular weights of the purified BDH subunit and holoenzyme were 44.

[Biosorption of chromium (VI) by waste biomass of epsilon-poly-L-lysine fermentation].

The sorption of hexavalent chromium by waste biomass of epsilon-Poly-L-lysine fermentation strains (Streptomyces albulus) PD-1 was studied. Effects of pretreatment ways, pH, initial concentration of Cr(VI), contact time and temperature on biosorption were determined. It was found that homogenization in HCl was the best way to pretreat mycelia, having an increased rate of Cr(VI) biosorption at 22.

Purification and characterization of a highly selective sucrose isomerase from Erwinia rhapontici NX-5.

A highly selective sucrose isomerase (SIase) was purified to homogeneity from the cell-free extract of Erwinia rhapontici NX-5 with a recovery of 27.7% and a fold purification of 213.6.

Propionic acid fermentation by Propionibacterium freudenreichii CCTCC M207015 in a multi-point fibrous-bed bioreactor.

Propionic acid was produced in a multi-point fibrous-bed (MFB) bioreactor by Propionibacterium freudenreichii CCTCC M207015. The MFB bioreactor, comprising spiral cotton fiber packed in a modified 7.5-l bioreactor, was effective for cell-immobilized propionic acid production compared with conventional free cell fermentation.

Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol.

The medium for one-step fermentation of raw inulin extract from Jerusalem artichoke tubers by Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol (R,R-2,3-BD) was developed. Inulin, K(2)HPO(4) and NH(4)Cl were found to be the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration was further investigated according to the Box-Behnken design and determined to be 77.