[Fusion expression, purification and bioactivity detection of human defensinalpha (HDalpha)].

Aim: To obtain recombinant human defensin alpha(HDalpha) and detect its biological activity, so as to facilitate further research.

Methods: The HDalpha gene fragment with hydroxylamine cleavage site was synthesized, and then cloned into pBV220-IL-4 vector to construct pBV220-IL-4-HDalpha. The constructed vector which was confirmed to be correct by sequencing was transformed into E.

Temperature- and pH-sensitive core-shell nanoparticles self-assembled from poly(n-isopropylacrylamide-co-acrylic acid-co-cholesteryl acrylate) for intracellular delivery of anticancer drugs.

Temperature- and pH-sensitive amphiphilic polymer poly(N-isopropylacrylamide-co-acrylic acid-co-cholesteryl acrylate) (P(NIPAAm-co-AA-co-CHA)) has been synthesized and employed to encapsulate paclitaxel, a highly hydrophobic anticancer drug, in core-shell nanoparticles fabricated by a membrane dialysis method. The nanoparticles are spherical in shape, and their size can be made below 200 nm by varying fabrication parameters. The lower critical solution temperature (LCST) of the nanoparticles is pH-dependent.

[Cloning and expression of a novel gene restin and preparation of antisera against the recombinant restin].

Aim: To clone a new human gene, restin, from retinoic acid-treated promyelocytic cell line HL-60, express the protein in E.coli and prepare the antisera against the protein.

Methods: The restin gene was amplified from retinoic acid-treated promyelocytic cell line HL-60 by RT-PCR and cloned into a prokaryotic expression vector.