Exogenous angiotensin (1-7) directly inhibits epithelial-mesenchymal transformation induced by transforming growth factor-β1 in alveolar epithelial cells.

Accumulating evidence indicates that angiotensin (1-7) [Ang-(1-7)] protects against idiopathic pulmonary fibrosis (IPF) in animal experiments. However, whether Ang-(1-7) effectively inhibits epithelial-mesenchymal transition (EMT) induced by transforming growth factor-β1 (TGF-β1) remains unclear. The aim of this study is to examine the eff ;ects of Ang-(1-7) on TGF-β1-induced EMT in human alveolar epithelial cells.

[Effects of tetramethylpyrazine on thrombin-induced tissue factor expression in vascular endothelial cells].

Objective: To observe the effects of tetramethylpyrazine (TMP) on tissue factor (TF) expression induced by thrombin in human umbilical vein endothelium derived cell line ECV304.

Methods: The changes in the total cellular procoagulant activity (PCA) of ECV304 cells exposed to thrombin were observed with one-stage clotting assay. TF mRNA expression in the exposed cells was examined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).

[Test particles of medical devices by Coulter Multisizer].

Introduced the "particles" item testing progress and some notes, using Coulter Multisizer, about medical devices and drugs wrapper, with an expectation of some help for the "particles" item tests in the solution in these area.

Levels of plasma des-gamma-carboxy protein C and prothrombin in patients with liver diseases.

Aim: To study the plasma des-gamma-carboxy protein C activity, antigen and prothrombin levels in patients with liver diseases and their clinical significance.

Methods: Plasma protein C activity (PC:C) was detected by chromogenic assay and antigen (PC:Ag) and des-gamma-carboxy protein C (DCPC) were detected by ELISA. Total prothrombin and unabsorbed prothrombin in plasma were detected by ecarin chromogenic assay.

[Changes of tissue factor and tissue factor pathway inhibitor in neonatal jaundice due to infection].

Objective: Tissue factor (TF) is an important factor in extrinsic coagulation. Tissue factor pathway inhibitor (TFPI) is a negative regulator of coagulation mediated by TF. Studies on TF and TFPI focus mainly on adult objects, seldom have been done on newborns, especially on sick newborns.

[Effect of angiotensin II on tissue factor expression in human peripheral blood monocytes and its mechanisms].

Objective: To elucidate the effect of angiotensin II (AngII) on the expression of tissue factor (TF) by monocytes and its mechanisms.

Methods: Monocytes were isolated from healthy volunteers by Ficoll-Hypaque gradient and Percoll, and cultured in RPMI-1640. Procoagulant activity (PCA) was determined by one-stage clotting method, TF antigen by ELISA.

[Observation on tissue factor pathway during the onset of acute cerebral infarction].

Objective: To establish the possible relationship between some coagulation factors and the onset of acute cerebral infarction (ACI).

Methods: The study population consisted of 71 patients with ACI confirmed by CT and 50 age-matched healthy volunteers. Blood samples were obtained during the onset period of ACI.