Publications by authors named "Xiao-dan Gai"
Objective: To observe the alleviative effects of preconditioning with diazoxide (DZ), a mitochondrial ATP-sensitive potassium channel opener, combined with subsequent hypothermia on anoxia-re-oxygenation injury.
Methods: Hippocampal neurons of new-born SD rat were cultured. DZ of the concentration of 250 micromol/L was added into the culture fluid.
Two types of acid-sensing ion channel (ASIC)-like currents in cultured rat hippocampal neurons were recorded and their characteristics were studied by using a whole-cell recording technique. The results revealed that the ASIC-like currents, induced by a quick drop of the extracellular pH, decayed with different time constants (tau) of 229 +/- 16 (Type I) and 1209 +/- 56 ms (Type II). The ASIC-like currents displayed different sensitivities to extracellular proton (pH0.
View Article and Find Full Text PDFAim: To investigate whether agmatine could selectively block a given type of the voltage-gated calcium channels (VGCC) and whether related receptors are involved in the blocking effect of agmatine on VGCC.
Methods: The whole-cell patch recording technique was performed to record VGCC currents in the cultured neonatal rat hippocampal neurons.
Results: Verapamil (100 micromol/L), a selective blocker of L-type calcium channel, significantly inhibited VGCC current by 80 %+/- 7 %.
Agmatine blocked voltage-gated calcium channel in cultured rat hippocampal neurons.
Acta Pharmacol Sin
August 2003
Aim: To investigate the mechanism of agmatine by observing the effect of agmatine on the voltage-gated channels in rat hippocampal neurons.
Methods: The whole-cell patch recording technique was performed to record the voltage-gated potassium, sodium, and calcium currents in cultured rat hippocampus. Agmatine was applied directly to the single neuron using a pressure injector with microtubules.