Abnormal lipid synthesis as a therapeutic target for cancer stem cells.

Cancer stem cells (CSCs) comprise a subpopulation of cancer cells with stem cell properties, which exhibit the characteristics of high tumorigenicity, self-renewal, and tumor initiation and are associated with the occurrence, metastasis, therapy resistance, and relapse of cancer. Compared with differentiated cells, CSCs have unique metabolic characteristics, and metabolic reprogramming contributes to the self-renewal and maintenance of stem cells. It has been reported that CSCs are highly dependent on lipid metabolism to maintain stemness and satisfy the requirements of biosynthesis and energy metabolism.

[Expression of secretoglobin family 2A member 2 in oral lichen planus lesions].

Objective: To examine the expression of secretoglobin family 2A member 2 (SCGB2A2) (mammaglobin A, MGB1) in oral lichen planus (OLP) lesions.

Methods: Sixteen OLP patients and 10 healthy controls were included in this study. The real time reverse transcription-PCR (RT-PCR), Western blotting and immunohistochemistry (IHC) were used to determine the mRNA and protein of SCGB2A2.

The dynamic changes of circulating OCN+ cells versus insulinlike growth factor-I during primary healing of orthognathic surgeries.

Objective: The objective of this study was to determine the dynamic changes of circulating osteocalcin(+) (OCN(+)) cells and insulinlike growth factor-I (IGF-I) in peripheral blood during early primary repair of jaw bones in patients with orthognathic surgery.

Study Design: The expression of bone-related genes was detected by RT-PCR in circulating OCN(+) cells. The numbers of OCN(+) cells and serum level of IGF-I were determined by flow cytometry, immunocytochemical staining, and ELISA.

[Correlation between the CD68 proportion of peripheral blood mononuclear cells and macrophage infiltration during acute rejection of rat oral mucosal xenotransplantation].

Objective: To investigate the role of the dynamic process of peripheral blood CD68 mononuclear cells proportion and macrophages inflitration and possible correlation between them during acute rejection of rat oral mucosal xenotransplantation.

Methods: Thirty-six female wistar rats were divided into three groups randomly, including xenotransplantation group (n = 15), trauma control group (n = 12) and normal control group (n = 9). The rat oral mucosa xenotransplantation model was established.

The correlation between the severity of radiotherapy-induced glossitis and endothelial cell injury in local tissues in a rat model.

Objectives: To explore the correlation between the severity of radiotherapy-induced glossitis (RTG) and endothelial cell injury in local tissues in a rat model.

Study Design: The RTG animal model was designed and used by our team. The Oral mucositis index(OMI) was documented daily.

The development and inflammatory features of radiotherapy-induced glossitis in rats.

Objectives: To improve the existing animal models (mice, rats, and hamsters) for radiotherapy-induced oral mucositis (RTOM), thereby establishing a radiotherapy-induced glossitis (RTG) Sprague-Dawley (SD) rat model.

Study Design: A lead device was designed to limit radiation exposure to a 1x1 cm2 area of a rat 's dorsal anterior tongue with a single 30 Gy of X-ray radiation. The general conditions of the irradiated rats, such as body-weight and behavior, were observed.

Neutrophils infiltration is early event of an oral mucosal xenotransplantation model.

Introduction: As important effector cells of the innate immune system, neutrophils are involved in rejection of solid organ and/or tissue transplants. But their role in rejection of oral mucosa transplantation (OMT) remains unclear. The aim of this study is to observe the spatial-temporal change of neutrophils during acute rejection of OMT.

Differential gene expression profiles of whole lesions from patients with oral lichen planus.

Background: The molecular biological properties of oral lichen planus (OLP) are largely unknown. The aim of this study was to identify the genes responsible for its pathogenesis at the genome scale using DNA microarray technology.

Methods: The RNA samples extracted from the specimens of nine OLP patients and nine controls were analyzed with Affymetrix GeneChip.

Simultaneous detection of IFN-gamma and IL-4 in lesional tissues and whole unstimulated saliva from patients with oral lichen planus.

Background: Although the roles of interferon (IFN)-gamma and interleukin (IL)-4 in oral lichen planus (OLP) have been described extensively in past decades, the available results are controversial. Moreover, few studies have utilized simultaneous detection of cytokines in local tissues and saliva to determine whether salivary cytokines could reflect the fact of local lesions.

Methods: IFN-gamma and IL-4 were determined simultaneously in lesions and whole unstimulated saliva (WUS) from OLP patients with various clinical forms.