Publications by authors named "Xiao-Zhong Shi"

Analysis of phosphorylation of YJL084c, a yeast protein.

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)

July 2002

PCL6, PCL7(PAP1), and PHO80 belong to the PHO80 subfamily of PCLs (PHO85 cyclins), share high homology in protein sequences, and function with some similarity. YLR190w, the substrate of PCL7-PHO85, shares homology with YJL084c in a 140-amino-acid region. In addition, YJL084c was reported as a PCL6-binding protein.

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[Phosphorylation of YLR190w by PAP1 PHO85 kinase complex].

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)

March 2002

A yeast two-hybrid screening by using PAP1 was performed to identify targets for PAP1-PHO85 cyclin-CDK complex. N-terminal fragment of protein YLR190w, a yeast gene encoding a 491 amino acids peptide, was identified, and its coding region was amplified by PCR. The interaction of PAP1 and YLR190w was confirmed by both two-hybrid assay and GST pull-down assay in vitro.

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