Publications by authors named "Xiao-Zhe Fu"

Mandarin fish (Siniperca chuatsi) been seriously harmed by infectious spleen and kidney necrosis virus (ISKNV) in recent years, but the early immune response mechanism of infection is still unknown. Here, we performed RNA sequencing on the spleens of mandarin fish infected with ISKNV at 0, 12, 24, 48, and 72 h post-infection (hpi) using short-read Illumina RNA sequencing and long-read Pacific Biosciences isoform sequencing to generate a full-length transcriptome. The immune responses of mandarin fish infected with ISKNV at the molecular level were characterized by RNA-seq analysis and weighted gene co-expression network analysis (WGCNA).

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Article Synopsis
  • Researchers identified and analyzed the cDNA and genomic DNA sequences of IL-6 and IL-6ST in mandarin fish (Siniperca chuatsi), revealing that their expression increases significantly in immune organs after infection with the infectious spleen and kidney necrosis virus (ISKNV).
  • A specific mutation in the IL-6 gene (1625C/T) has been linked to increased resistance against ISKNV, suggesting potential genetic markers that could aid in breeding disease-resistant mandarin fish.
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As a high mortality disease, Infectious spleen and kidney necrosis virus (ISKNV) can cause massive economic damage on mandarin fish farming industry in China, which seriously hindered the development of mandarin fish farming industry. In this research, SWCNTs (single-walled carbon nanotubes) as a candidate for DNA vaccine carrier was vaccinated by immersion (1, 2, 5, 10, 20 mg/L) in juvenile mandarin fish. In muscle, spleen and kidney tissues, the results showed that transcription and expression of MCP gene can be detected in pcDNA-MCP and SWCNTs-pcDNA-MCP groups after bath immunization.

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Infectious spleen and kidney necrosis virus (ISKNV) cause a high mortality disease which lead to significant economic loss on mandarin fish in China. There is no effective drug or vaccine against this fatal disease at present. Meanwhile, many drugs and vaccines had no effect in many cases account of several impenetrable barriers (cell, skin and gastrointestinal tract).

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As a key component of life science, bioinformatics has been widely applied in genomics, transcriptomics, and proteomics. However, the requirement of high-performance computers rather than common personal computers for constructing a bioinformatics platform significantly limited the application of bioinformatics in aquatic science. In this study, we constructed a bioinformatic analysis platform for aquatic pathogen based on the MilkyWay-2 supercomputer.

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In order to find changes in mortality and immunological variables of Litopenaeus vannamei parents and the filial WSSV-resistant and -susceptible families after infection with WSSV under different experimental conditions, the haemolymph total haemocyte count (THC), phenoloxidase (PO), and superoxide dismutase (SOD) activities were measured at days 0, 1, 3, 6, 9, 12 and 15 after challenge and shrimp mortality was also recorded. When shrimps were challenged with 10(-3) (1.29x10(6)copiesmL(-1)), 10(-4) (1.

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  • The major capsid protein of the lymphocystis disease virus (LCDV-rc) from the fish Rachycentron canadum was amplified and analyzed to understand its genetic makeup.
  • A 457bp DNA core fragment was successfully amplified using specially designed primers, and the complete sequence was reconstructed using inverse PCR.
  • The study found that the gene has an open reading frame of 1380bp, encoding a protein of 459 amino acids, and phylogenetic analysis confirmed that LCDV-rc is closely related to other Lymphocystis viruses, classifying it as Lymphocystivirus.
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