[The relationship between CD4+ CD25+ regulatory T cell in the peripheral blood and poor or nonresponsiveness to hepatitis B vaccine].

Objective: To investigate CD4+ CD25+ regulatory T cell frequencies in the peripheral blood of poor or non-responsiveness to Hepatitis B vaccine, and try to understand the relationship between CD4+ CD25+ regulatory T cell and poor or non-responsiveness to Hepatitis B vaccine.

Methods: Flow cytometric analysis was employed for CD4+ CD25+ regulatory T cell frequencies in the peripheral blood of 25 cases of non-responsiveness, 30 cases of poor-responsiveness, and collected 20 cases of responsiveness as control.

Results: CD4+ CD25+ regulatory T cell frequencies of responsiveness was (4.

[Promotion of the activity of Akt in CD8+ T cells by the crosslink of 3H3, an agonist antibody of 4-1BB].

Aim: To investigate whether 4-1BB could promote the proliferation and survival of CD8(+) T cells proliferation, and the activity of protein kinase B (Akt).

Methods: Highly purified CD8(+) T cells were separated and stimulated by CD3 antibody. The crosslink of 3H3 and the proliferation and antiapoptosis of CD8(+) T cells were examined.