Background: Regenerating gene (REG) family proteins play a pivotal role in cell proliferation, tissue regeneration, and tumor metastasis. Recent studies have concentrated on the role of REG proteins in pancreatic cancer, but the results remain controversial. In this study, a meta-analysis was performed to evaluate the precise diagnostic value of REG proteins in pancreatic cancer.
View Article and Find Full Text PDFTwo new monoterpene esters, illigerates H and I ( and ), and six known compounds actinodaphine (), bulbocupnine (), stephanine (), hypserpanine B (), betulinic acid () and gallic acid () were obtained from the root of Dunn. Their structures were elucidated by spectroscopic analysis. Anti-inflammatory and α-glucosidase inhibitory activity of some isolated compounds were assessed.
View Article and Find Full Text PDFWe present a chip-scale integrated pH sensor with high sensitivity by using an optofluidic ring resonator (OFRR) laser. An optical fiber with a high refractive index (RI) is employed both as an optical cavity and the sensing reactor along a microchannel, while disodium fluorescein (DSF) aqueous solution with a low RI is served as the cladding gain medium and fluorescent probes. The pump light is introduced along the fiber axis and guided by the total internal reflection at the fiber/cladding interface.
View Article and Find Full Text PDFThree new flavonoids, quercetin-3--6-[methyl-()-3-hydroxy-3-methylglutaroyl(1→6]-β-d-glucopyranoside (), kaempferol-3--[methyl-()-3-hydroxy-3-methylglutaroyl(1→6)]-β-d-glucopyranoside (), and quercetin-3--6-[()-4-methoxy-5-methylhexa-2,4-dienoatyl(1→6)]-β-d-glucopyranoside (), and two new alkaloids, 5-dehydroxymethyl-pyrrolemarumine 4″--α-l-rhamnopyranoside () and -methyl--((4--α-l-rhamnopyranoside)benzyl) oxalamide (), together with 45 known compounds (-) were isolated from the leaves of Lam. Among those compounds, 1-octacosanol (), a straight-chain 28-carbon alcohol, exhibited good activity against diphenoxylate-induced constipation in mice, which is obtained as a laxative constituent from the plant for the first time. In order to have an accurate understanding of the content of compound , a quantification with gas chromatography-tandem mass spectrometry (GC-MS/MS) was carried out.
View Article and Find Full Text PDFTwo new monoterpene esters illigerates F and G ( and ) together with 5 know compounds illigerate A (), illigerate C (), actinodaphnine (), N-methylactinodaphnine() and N-methyllaurotetanine() were isolated from S. Z. Huang et S.
View Article and Find Full Text PDFJ Asian Nat Prod Res
June 2019
Phytochemical investigation on Hemiphragma heterophyllum led to the isolation of two new compounds, heterophyllumin A (1) and heterophylliol (3), along with nine known compounds, (‒)-sibiricumin A (2), iridolactone (4), jatamanin A (5), dihydrocatalpolgenin (6), 25-hydroperoxycycloart-23-en-3β-ol (7), 24-methylenecycloartanol (8), (+)-pinoresinol (9), hexadec-(4Z)-enoic acid (10), and 9,12, 15-octadecatrienoic acid (11). Their structures were elucidated on the basis of detailed spectroscopic analyses and by comparison with literature data. Further, the structure of compound 3 was unambiguously confirmed by single-crystal X-ray analysis.
View Article and Find Full Text PDFFour new coumestans dolichosins A - D (1: -4: ) were isolated from the roots of , together with four known compounds: isosojagol (5: ), phaseol (6: ), psoralidin (7: ), and 4″,5″-dehydroisopsoralidin (8: ). Their structures were elucidated on the basis of spectroscopic data interpretation, mass spectrometric analyses, and the comparison with literature data of related compounds. The anti-inflammatory activity of these compounds (1: -8: ) was evaluated through the inhibition of nitric oxide production in lipopolysaccharide-activated murine macrophage RAW 264.
View Article and Find Full Text PDFSerum exosomal microRNAs (miRNAs) have received considerable attention as potential biomarkers for tumor diagnosis. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) is commonly used to detect miRNA expression levels in various types of cancer. One prerequisite for valid RT‑qPCR data is the correct normalization of miRNAs to stably expressed endogenous reference genes (RGs).
View Article and Find Full Text PDFThe polarization characteristics of Whispering-Gallery-Mode (WGM) fiber lasers based on evanescent-wave-coupled gain are investigated. For the laser gain is excited by side-pumping scheme, it is found that the polarization property of lasing emission is simply dependent on the polarized states of the pump beams. The polarization property of lasing emission depends on the propagating situation of the pump beams in an optical fiber if the laser gain is excited by evanescent-wave pumping scheme, that is, if the pump beams within the fiber are meridional beams, the lasing emission is a transverse electric (TE) wave that forms a special radial polarization emission.
View Article and Find Full Text PDFTo investigate the expression of hPOT1 in the HeLa cell line and screen point mutations of hpot1 in different tumor tissues a two step osmotic method was used to extract nuclear proteins. EMSA was performed to determine the expression of hPOT1 in the HeLa cell line. PCR was also employed to amplify the exon14 sequence of the hpot1 gene in various of cancer tissues.
View Article and Find Full Text PDFXi Bao Yu Fen Zi Mian Yi Xue Za Zhi
December 2010
Aim: To observe the anti-inflammatory effect of adenovirus-mediated sTLR2 in LPS-induced murine inflammation, and to provide a new thinking and tactics of prevention and treatment for acute inflammatory reaction.
Methods: Murine inflammation model induced by LPS (lethal dose) was established. BALB/c mice were divided into normal control group, inflammation model group and AdTLR2 treatment group.
Based on the investigation in Nanji Islands sea area of Zhejiang Province from April 2006 to March 2007, the dynamics of Alexandrium tamarense and its relations with environmental factors were studied. In the study sea area, A. tamarense cells could only be found in spring (from April to June), and the cell abundance was of single peak curve, with the highest (12250 cells x L(-1)) in surface water on 8th of May.
View Article and Find Full Text PDFGuang Pu Xue Yu Guang Pu Fen Xi
December 2008
Lasers with spherical or cylindrical dielectric resonators supported by whispering gallery modes (WGM) have attracted much interest due to their microscopic size, high cavity Q factor, and low lasing threshold. Cylindrical microcavity lasers based on the gain only in the evanescent field region of whispering gallery modes have been demonstrated in our recent works. The gain was excited by the evanescent wave of longitudinal optical pumping along the optical fiber.
View Article and Find Full Text PDFA rapid and sensitive chemiluminescence microfluidic immunoassay system based on super-paramagnetic microbeads for determination of alpha-fetoprotein (AFP) is described. In this system we use CO(2) laser to fabricate microfluidic chip, and use super-paramagnetic microbeads as solid carrier of antibody, chemiluminescence as detection signal. With this system we can perform AFP analysis within 20 min, and the linear range of AFP concentration is 1 approximately 800 ng/mL, the detection limit is 0.
View Article and Find Full Text PDFZhongguo Yi Liao Qi Xie Za Zhi
September 2006
We use direct-write laser micromachining technology to fabricate the microfluidic chip, and to establish a microfluidic chemiluminescence immunoassay system based on superparamagnetic microbeads, for detecting alpha- fetoprotein (AFP). The AFP analysis can be completed in 20 minutes with 5 microl sample and 5 microl reagent, and there is a good linear correlation in the range of 1-800 ng/ml.
View Article and Find Full Text PDFSister chromatids separation is under precise regulation during cell cycle. Any turbulence happened in the separation process can cause instability in the transmission of inherited material, and may cause: death or disease of cell or even individual. In eukaryotic cells, one conserved mechanism governs the separation of sister chromatids.
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