Mitochondrial DNA A DNA Mapp Seq Anal
September 2016
Mitochondrial DNA A DNA Mapp Seq Anal
September 2016
The complete mitochondrial genome of the Sara Longwing Heliconius sara has been reconstructed from the whole-genome Illumina sequencing data. The mitochondrial genome is 15,372 bp in size with the highly asymmetric overall A + T content of 80.6%.
View Article and Find Full Text PDFMitochondrial DNA A DNA Mapp Seq Anal
November 2016
The complete mitochondrial genome of the wild silk moth Bombyx huttoni has been reconstructed from the whole-genome Illumina sequencing data. The circular genome was determined to be 15,638 bp in length, consisting of 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one A+T-rich D-loop or control region. Twelve PCGs (COX2, COX3, CYTB, ND1, ND2, ND3, ND4, ND4L, ND5, ND6, ATP6, and ATP) are initiated with the typical ATN codons, whereas COX1 was tentatively determined to use the unusual CGA as its start codon.
View Article and Find Full Text PDFZhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
July 2002
Objective: To study the effect of two cytokines, basic fibrolast growth factor(bFGF) and insulin-like growth factor-I(IGF-I), on cell proliferation in chondrocytes of adult rabbits.
Methods: The primary chondrocytes of adult rabbits were harvested and cultured with bFGF and IGF-I at different concentrations, respectively, as well as with the mixture of the two cytokines; the quantity of cultured chondrocytes was detected by MTT assay at the 24th, 48th and 72nd hours; and the final fold increase of different groups was measured by cell count for the 3rd passage; and the proliferation index of the groups was recorded by flowing cytometer on the 14th day.
Results: 1.