[Effect and mechanism of miR-124 on osteogenic differentiation of dental pulp mesenchymal stem cells].

Purpose: To evaluate the effect of microRNA (miR)-124 on osteogenic differentiation of dental pulp mesenchymal stem cells (DPSCs) and to explore the possible mechanism.

Methods: Logarithmic DPSCs were collected and divided into blank group, no-load group, miR-124 inhibitor group, miR-124 inhibitor combined with N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-ph (DAPT, Notch signaling pathway inhibitor) group. The blank group was not treated, the empty group was transfected with negative control vector inhibitor-NC, the miR-124 inhibitor group was transfected with miR-124 inhibitor, the miR-124 inhibitor combined with DAPT group was transfected with miR-124 inhibitor, and DAPT was added to make the final concentration of 5 μmol/L.

[Prevalence of malocclusion among 5 387 12- to 14-year-old adolescents in Jiangxi province, China: an epidemiological study].

Objective: We provided baseline data for oral public health through epidemiological surveys to investigate the prevalence of malocclusion and orthodontic treatment proportion in 12- to 14-year-old adolescents in Jiangxi province, China.

Methods: Multi-stage random cluster sampling was used. A total of 5 387 12- to 14-year-old adolescents were examined by three professional dentists according to Angle's classification of malocclusion.

[Status and strategies of oral health service demand and medical treatment utilization among 3- to 5-year-old preschool children in Jiangxi province].

Objective: This study aimed to investigate the oral health service needs and dental treatment among 3-year-old to 5-year-old preschool children in Jiangxi province and provide data to support the development of relevant oral health policies, enhance the oral health service utilization and visit rate, and optimize oral manpower and service resources.

Methods: A total of 2 880 preschool children were selected via stratified cluster sampling. Dental examination methods and criteria according to the fourth national oral health epidemiological survey were adopted.

[Grape seed proanthocyanidin extracts inhibit lipopolysaccharide of Porphyromonas gingivalis].

Purpose: To study the inhibitory effect of grape seed proanthocyanidin extracts on 1ipopolysacharides of Porphyromonas gingivalis.

Methods: Grape seed proanthocyanidin extracts were double-diluted to different concentrations by two-fold dilution. The effect on Porphyromonas gingivalis was measured by minimal inhibitory concentration (MIC).

[Application of participatory teaching mode in oral health education].

Purpose: To apply participatory teaching mode in oral health education, and to assess its role in cultivating comprehensive stomatological professionals suitable for the development of modern medicine.

Methods: Sixty undergraduate students from grade 2005 in Stomatological College of Nanchang University were selected. Among those students, oral health education course was carried out by traditional teaching mode, while 120 undergraduate students from grades 2006 to 2007 received participatory teaching approach, which paid attention to practice in oral health education practice course.

[The application of desocclusol and manual mechanical method in endodontic retreatment].

Purpose: To investigate the clinical method to remove a gutta-percha and root canal sealers.

Methods: 168 root canals which had been filled with gutta-percha were selected,all root canals needed endodontic retreatment.The root canal fillings were removed and the root canal was re-prepared with the method of desocclusol and manual mechanical method.

[Dental caries and periodontal diseases in adult Jiangxi cohort: a sample investigation].

Purpose: To investigate the current status of dental caries and periodontal diseases in the population aged 35-44 and 65-74 years in Jiangxi province, and to provide information for prevention of these diseases.

Methods: 1584 people (aged 35-44 and 65-74 years) were selected by equal-sized stratified multi- stage and random sampling. Their dental caries and periodontal diseases were examined in routine epidemiologic surgery manner.