Autophagy Mediates MMP-2 Expression in Glaucomatous Trabecular Meshwork Cells.

Purpose: To investigate the effect of 3-methyladenine (3-MA) and starvation on the expression of matrix metalloproteinase (MMP-2) in patients with primary open-angle glaucoma.

Methods: Primary TM cells were cultured and divided into three groups. The control group was treated with a normal medium, the 3-MA group was stimulated with 3-MA, and the starvation group received nutrient depletion by replacing the normal media with Earle's balanced salt solution.

Pre-cardiopulmonary bypass administration of dexmedetomidine decreases cardiac troponin I level following cardiac surgery with sevoflurane postconditioning.

Objective: This study was performed to determine the effect of dexmedetomidine (DEX) administration on myocardial damage in cardiac surgery with sevoflurane postconditioning.

Methods: We retrospectively examined all cardiac valve replacement surgeries from 1 April 2016 to 30 April 2017. Eligible patients were divided into two groups based on whether DEX was infused.

Controllable assembly of a three-dimensional metal-organic supramolecular framework displaying hydrogen-bonding and π-π stacking interactions.

The complex poly[[aqua(μ2-phthalato-κ(2)O(1):O(2)){μ3-2-[3-(pyridin-2-yl)-1H-pyrazol-1-yl]acetato-κ(4)N(2),N(3):O:O'}{μ2-2-[3-(pyridin-2-yl)-1H-pyrazol-1-yl]acetato-κ(3)N(2),N(3):O}dizinc(II)] dihydrate], {[Zn2(C10H8N3O2)2(C8H4O4)(H2O)]·2H2O}n, has been prepared by solvothermal reaction of 2-[3-(pyridin-2-yl)-1H-pyrazol-1-yl]acetonitrile (PPAN) with zinc(II). Under hydrothermal conditions, PPAN is hydrolyzed to 2-[3-(pyridin-2-yl)-1H-pyrazol-1-yl]acetate (PPAA(-)). The structure determination reveals that the complex is a one-dimensional double chain containing cationic [Zn4(PPAA)4](4+) structural units, which are further extended by bridging phthalate ligands.

[Efficacy of continuous propofol infusion via the common carotid artery for general anesthesia].

Objective: To investigate the efficacy of continuous propofol infusion via the common carotid artery for general anesthesia.

Methods: Forty adult patients scheduled for abdominal surgery were randomly assigned into 2 groups to receive propopol via the common carotid artery (IC group, n=20) or via the median cubital vein (IV group, n=20). Anesthesia was induced with intravenous administration of drugs and maintained with continuous propofol infusion via the common carotid artery or the median cubital vein, with the CSI stabilized at 40-/+5 till the end of the operation.

[Protective effects of Huanglian Jiedu decoction on vascular endothelial function in type 2 diabetic rats].

Objective: To investigate the effects of Huanglian Jiedu (HLJD) decoction on vascular endothelial function in type 2 diabetic rats and explore the prophylactic and therapeutic significance and pharmacological mechanism of HLJD decoction in type 2 diabetic angiopathic complication.

Method: The murine type 2 diabetes models were induced by the intravenous injection of a small dose of streptozotocin plus high fat and high caloric laboratory chow. Then modeled diabetic animals were divided into model group, HLJD group, and aspirin group.

[Thinking of acupuncture and moxibustion slimming treatment].

The authors think the problems at present in clinical slimming treatment of acupuncture and moxibustion, such as the effect and the mechanism of slimming, local slimming and the rebounding of body weight after slimming therapy, etc.. Holding that acupuncture and moxibustion slimming therapy has a certain therapeutic effect, acupuncture and moxibustion treatment combined with proper control of diet, sports and other methods can increase the effect of reduction of body weight; and regulation of the satiety center, inhibition of excessive appetite and mobilization of decomposition of lipids are an ideal program for acupuncture and moxibustion slimming treatment; local slimming treatment should be conducted on the basis of general slimming treatment; regulation of the satiety center, scientific combination of diet and proper sports are a tactic for prevention from rebounding of body weight after slimming treatment.

[Experimental study on expression of connexin 43 in meridians of rats].

Objective: To probe into expression of connexin 43 (Cx43) in the meridians of the normal healthy rats and the relation among connexin, gap junction and meridian.

Methods: PowerVision two step immunohistochemical technique and ASIAS-2000 automatical image-scan analyzing system were used to detect Cx43 level and distribution in the Kidney and Bladder Meridians of the rat.

Results: Cx43 expressed mainly in skin epithelia, fibroblasts and mast cells of the subcutaneous layer.

[The effect of restrained stress on cardiac myocyte apoptosis in rats and Chinese herbs yixinning interference on it].

Aim: To discuss the effects of restrained stress on cardiac myocyte apoptosis of rats in the whole body, and the effect of anti-stress-induced cardiac myocyte injury treated by Chinese herbs yixinning.

Methods: Agarose gel electrophoresis and TUNEL are used to detect cardiac myocyte apoptosis.

Results: (1) When restrained stress 1,2,4 week, the DNA ladder of stress groups was negative, while in situ apoptosis of stress groups increased apparently compared with control group (P < 0.