[Characterizing Dissolved Organic Matter (DOM) in Wastewater from Scale Pig Farms Using Three-Dimensional Excitation-Emission Matrices (3DEEM].

The properties of material composition in swine wastewater are closely related to its potential environmental effects, and it could provide theoretical bases for formulating scientific resource management measures to study the composition of organic matter in wastewater. In the present study, swine wastewater was directly collected from waste-retention basins in various scale pig farms with a different farming scale (based on the annual pig slaughter), namely Cheng Lin (CL, 5 000), Wu Yang-gao (WYG, 2 000), Wan Gu (WG, 20 000), and Zhang Bang (ZB, 24 000), located in Yujiang County of Jiangxi Province. The main purpose of this study was to characterize dissolved organic matter (DOM) in swine wastewater using three-dimensional excitation-emission matrices (3DEEM) and parallel factor analysis (PARAFAC).

[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].

More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater. In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment. The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.

Basic fibroblast growth factor lentiviral vector-transfected sheep bone marrow mesenchymal stem cells and non-specific osteogenic gene expression.

In the present study, sheep bone marrow stem cells (BMSCs) were transfected with basic fibroblast growth factor (bFGF)-lentivirus in order to investigate the influence of bFGF on osteogenic gene expression. bFGF‑transfected BMSCs (experimental group) and non-transfected BMSCs (control group) were also transfected with a green fluorescent protein gene in order to measure the transfection efficiency of the (bFGF)-lentivirus using flow cytometry. Using quantitative polymerase chain reaction assays, the changes in expression from three genes (osteopontin, OPN; osteocalcin, OC; and collagen-I) in BMSCs from the experimental and control groups were measured.