Publications by authors named "Xiao-Wen Yang"

Nine undescibed abietane diterpenoid alkaloids (DAs), salviamines G‒H (1-2), isosalviamines G‒J (3-6), and miltiorramines A‒C (7-9) were isolated from the roots of Salvia miltiorrhiza. Their chemical structures including absolute configurations were elucidated by extensive spectroscopic analysis (including 1D and 2D NMR, and HRESIMS), combined with the calculated ECD method and single-crystal X-ray diffraction analysis. Among them, compounds 1-6 are unusual 20-nor- or 19,20-bisnor-abietane DAs with an oxazole ring.

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Saponins are bioactive components of many medicinal plants, possessing complicated chemical structures and extensive pharmacological activities, but the production of high-value saponins remains challenging. In this study, a 6'-O-glucosyltransferase PpUGT7 (PpUGT91AH7) was functionally characterized from Paris polyphylla Smith var. yunnanensis (Franch.

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Introduction: Steroidal saponins characterised by intricate chemical structures are the main active components of a well-known traditional Chinese medicine (TCM) Rhizoma Paridis. The metabolic profiles of steroidal saponins in vivo remain largely unexplored, despite their renowned antitumor, immunostimulating, and haemostatic activity.

Objective: To perform a comprehensive analysis of the chemical constituents of Rhizoma Paridis total saponins (RPTS) and their metabolites in rats after oral administration.

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Article Synopsis
  • Ochratoxin A (OTA) is a harmful substance found in many foods, requiring effective detection methods for safety and health.
  • Two new aptasensors, OTABA4 and OTABA3, were developed using modified OTA-binding aptamers that enhance the detection of OTA by increasing fluorescent signals when OTA is absent.
  • The updated systems demonstrated significantly improved sensitivity in detecting OTA, with detection limits of 0.28 ng/ml for OTABA4 and 0.059 ng/ml for OTABA3, and performed effectively in testing corn and peanut samples for food safety.
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Article Synopsis
  • Ochratoxin A (OTA) is a dangerous mycotoxin found in food that can harm human health, prompting the need for effective detection methods.
  • This study introduces a highly sensitive aptasensor using a G-rich DNA sequence (G43) combined with thioflavin T (ThT), which improves fluorescence for detecting OTA through a hybridization chain reaction (HCR).
  • The aptasensor operates by utilizing a hairpin-locked aptamer (HL-Apt) that changes structure when OTA binds, triggering a reaction that enhances fluorescence, with a linear response to OTA concentrations and a very low detection limit of 0.008 ng/mL.
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Duchenne/Becker muscular dystrophy (DMD/BMD) is one of the most common progressive muscular dystrophy diseases with X-linked recessive inheritance. It is mainly caused by the deletion, duplication and point mutation of gene. In rare cases, it is also caused by the destruction of gene by chromosomal structural rearrangement.

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Highly conserved MutS and MutL homologs operate as protein dimers in mismatch repair (MMR). MutS recognizes mismatched nucleotides forming ATP-bound sliding clamps, which subsequently load MutL sliding clamps that coordinate MMR excision. Several MMR models envision static MutS-MutL complexes bound to mismatched DNA via a positively charged cleft (PCC) located on the MutL N-terminal domains (NTD).

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Novel magnetic and fluorinated porous carbons (M-FPCs) with high fluorine content, large pore volume and specific surface area were first prepared by carbonizing and further fluorinating Fe-Zr bimetal-organic frameworks. The M-FPCs exhibit excellent adsorption performance toward perfluorinated compounds (PFCs), and the maximal adsorption capacity ranges from 518.1 to 919.

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One-dimensional (1D) sliding of DNA-binding proteins has been observed by numerous kinetic studies. It appears that many of these sliding events play important roles in a wide range of biological processes. However, one challenge is to determine the physiological relevance of these motions in the context of the protein's biological function.

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Background: Brucellosis is an infectious-allergic zoonotic disease caused by bacteria of the genus Brucella. Early diagnosis is the key to preventing, treating, and controlling brucellosis. Fluorescence polarization immunoassay (FPA) is a new immunoassay for relatively rapid and accurate detection of antibodies or antigens based on antigen-antibody interaction.

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Background: The prevalence of human brucellosis in Qinghai Province of China has been increasing rapidly, with confirmed cases distributed across 31 counties. However, the epidemiology of brucellosis transmission has not been fully elucidated. To characterize the infecting strains isolated from humans, multiple-locus variable-number tandem repeats analysis (MLVA) and whole-genome single-nucleotide polymorphism (SNP)-based approaches were employed.

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Background: Lingual linear lesions (LLLs) are the oral linear lesions located on the dorsum, lateral borders, and/or ventral surface of tongue. It has been suggested that LLLs might be an early clinical sign of vitamin B deficiency. Here, a retrospective study was conducted to further investigate and validate the association between LLL and vitamin B deficiency.

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Background: Oral lichen planus (OLP) is a relatively common mucocutaneous disorder, and its causative factors and pathogenesis are not fully understood. Existing studies on the association between hematinic deficiencies and OLP are limited and inconsistent. The aim of this study was to assess the hematinic deficiencies in a cohort of OLP patients and evaluate the correlation between hematinic deficiencies and OLP.

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Objective: To investigate the effect of down-regulation of SND1 expression on senescence of human diploid fibroblasts.

Methods: Western blot and immunohistochemistry were used to detect the expression of SND1 in young or senescent 2BS cells and aged tissues. Immunofluorescence was conducted to detect the localization of SND1 in young 2BS cells.

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To study the changing of characteristics and formation mechanisms of PM in Beijing during the last two years, particulate matter concentrations, weather conditions, and air-mass trajectories were analyzed during severe pollution episodes in fall and winter 2016-2017 using routine observations and the TrajStat model. Results showed that 13 heavy pollution events, each lasting at least two days, occurred in Beijing. Of these, approximately 61.

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Research Question: What is the genetic aetiology of three resistant ovary syndrome (ROS) pedigrees from 13 Chinese Han families with non-syndromic premature ovarian insufficiency (POI).

Design: The proband in each family was subjected to whole-exome sequencing. Bioinformatic and in-vitro functional analyses were performed for the functional characterization of the FSHR mutations.

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Background: Blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) is a malformation of the eyelids. Forkhead Box L2 (FOXL2) is the only gene known to be associated with BPES.

Methods: We identified two Han Chinese BPES families with premature ovarian insufficiency (POI).

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Resistant HCV variants carrying NS5B S282T mutation confer reduced sensitivity to sofosbuvir, the sole marketed NS5B polymerase inhibitor. On the basis of the finding that 2'-α-F-2'-β-C-methylcytidine 5'-triphosphate (8) was more potent than sofosbuvir's active metabolite on inhibition of both wild-type and S282T mutant polymerase, a dual-prodrug approach has been established. Twenty-nine phosphoramidates with N-modified cytosine were designed, synthesized, and evaluated for anti-HCV activity.

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In this study, detailed activity level of typical sector in Chengde in 2013 was obtained through a full-coverage investigation. A comprehensive emission inventory with country-level resolution in 2013 was developed based on guide of atmospheric pollutant emission inventory and updated emission factors. Then, the emission inventory within 1 km×1 km grid was generated using source-based spastial surrogates including population, road network and landuse date.

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The complete sequence mitochondrial genome of Papilio polytes was determined using long PCR and conserved primers walking approaches. The genome was 15,260 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of P.

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The complete mitochondrial genome sequence of Papilio bianor was determined in the present paper. The complete mtDNA from P. bianor was 15,358 base pairs in length and contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes and a control region.

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The complete sequence mitochondrial genome of Takydromus sexlineatus was determined using long PCR and conserved primers walking approaches. The genome was 18,943 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of T.

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By using the method of fixed-pointed monitoring in streams of two small watersheds in Three Gorges Reservoir Area, the contents of dissolved silicon (DSi), dissolved inorganic nitrogen (DIN) and dissolved phosphorus (DP) are analyzed. The seasonal variation, the content ratio of DSi, DIN and DP, and the potential environmental problems are also discussed. The results indicate that: (10 In the two rivers, DSi content ranges from 0.

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