Single-cell transcriptome sequencing analysis of physiological and immune profiling of crucian carp (Carassius auratus) gills.

Gills are the main respiratory organs of fish and bear important physiological and immunological functions, but the functional heterogeneity of interlamellar cell mass (ILCM) at the single-cell level has rarely been reported. Here, we identified 19 cell types from the gills of crucian carp (Carassius auratus) by single-cell RNA sequencing (scRNA-seq) in combination with histological analysis. We annotated ILCM and analyzed its functional heterogeneity at the single-cell level for the first time.

Achieving functional recovery without anatomical repositioning: A case of corneal flap inversion trauma post femtosecond laser-assisted in situ keratomileusis surgery.

Post femtosecond laser-assisted in situ keratomileusis corneal flap inversion trauma is an exceptionally rare and challenging complication in ophthalmology, highlighted by a case involving a 29-year-old woman who suffered an eye injury from ice chips 10 months after undergoing bilateral femtosecond laser-assisted in situ keratomileusis surgery. The injury led to a corneal flap inversion and melting, exacerbated by a delay in seeking treatment due to the pandemic. When she finally presented for medical attention, her vision was severely compromised, with dense epithelial ingrowth obstructing the visual axis or blocking the pupillary entrance.

Synthesis, Anticancer Activity, Structure-Activity Relationship and Mechanistic Investigations of Falcarindiol Analogues.

Forty samples of optically active falcarindiol analogues are synthesized by using the easily available C2 symmetric (R)- and (S)-1,1'-binaphth-2-ol (BINOL) in combination with Ti(O Pr) , Zn powder and EtI. Their anticancer activities on Hccc-9810, HepG2, MDA-MB-231, Hela, MG-63 and H460 cells are assayed to elucidate their structure-activity relationships. These results showed that the falcarindiol analogue (3R,8S)-2 i with the terminal double bond has the most potent anti-proliferation effect on Hccc-9810 cells with IC value of 0.

Synthesis and Biological Evaluation of Falcarinol-Type Analogues as Potential Calcium Channel Blockers.

A series of enantiomers of falcarinol analogues () were synthesized using a chiral 1,1'-binaphth-2-ol (BINOL)-based catalytic system. The neuroprotective effects of falcarinol () and its analogues () on PC12 cells injured by sodium azide (NaN) were investigated. The structure-function relationships and possible mechanism were studied.

Experimental study of trabecular tissue repair for corneal defect in rabbits.

Aim: To investigate the mechanism and effect of trabecular tissue repair for corneal defect, and to provide a theoretical basis for its clinical application.

Methods: Trabeculectomy was performed on 40 (80 eyes) of 70 New Zealand white rabbits. Take trabecular tissue for backup.

Predicting Gram-Positive Bacterial Protein Subcellular Location by Using Combined Features.

There are a lot of bacteria in the environment, and Gram-positive bacteria are the most common ones. Some Gram-positive bacteria are very harmful to the human body, so it is significant to predict Gram-positive bacterial protein subcellular location. And identification of Gram-positive bacterial protein subcellular location is important for developing effective drugs.

[Study on the expression level of four kinds of interleukins in mice model with peri-implantitis].

Purpose: To investigate the gross manifestation and tissue destruction at different inflammatory periods of ligature-induced peri-implantitis in mice model, and to evaluate the changes of interleukins level in the gingival crevicular fluid of implants at different stages.

Methods: Forty 4-week-old male C57BL/6N mice (18-22 g) were used and the maxillary right first molars were extracted, custom-made pure titanium machined screw type implants (1 mm×1.5 mm) were placed immediately, one implant per animal.

Mechanism of interaction between ocular and nasal neurogenic inflammation in allergic rhinoconjunctivitis.

Purpose: The mechanisms of naso-ocular interaction in allergic rhinoconjunctivitis are not well understood. Neurogenic inflammation affects both eyes and nose via the same neurogenic factors. The purpose of this study was to investigate the effects of neurogenic inflammation on conjunctival inflammation following nasal allergen provocation.

Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells.

Aim: To explore the possibility of human umbilical cord mesenchymal stem cells (hUCMSCs), human umbilical vein endothelial cells (hUVECs), human dental pulp stem cells (hDPSCs) and human periodontal ligament stem cells (hPDLSCs) serving as feeder cells in co-culture systems for the cultivation of limbal stem cells.

Methods: Different feeder layers were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 and were treated with mitomycin C. Rabbits limbal stem cells (LSCs) were co-cultured on hUCMSCs, hUVECs, hDPSCs, hPDLSCs and NIH-3T3, and then comparative analysis were made between each group to see their respective colony-forming efficiency (CFE) assay and immunofluorescence (IPO13,CK3/12).

Observation on ultrastructure and histopathology of cornea following femtosecond laser-assisted deep lamellar keratoplasty for acute corneal alkaline burns.

Aim: To demonstrate the changes in ultrastructure and histopathology of the cornea in acute corneal alkaline burns after femtosecond laser-assisted deep lamellar keratoplasty.

Methods: The New Zealand white rabbits treated with alkaline corneal burn were randomized into two groups, Group A (16 eyes) with femtosecond laser-assisted deep lamellar keratoplasty 24h after burn and Group B (16 eyes) without keratoplasty as controls. All eyes were evaluated with transmission electron microscopy (TEM) at 1, 2, 3, and 4wk follow-up, then all corneas were tested by hematoxylin and eosin staining histology.

Use Videostrobokymography to Quantitatively Analyze the Vibratory Characteristics Before and After Conservative Medical Treatment of Vocal Fold Leukoplakia.

Objectives: To quantitatively analyze the vibratory characteristics of vocal folds before and after conservative treatments to evaluate the outcomes of conservative treatments for vocal fold leukoplakia using videostrobokymography (VSK).

Study Design: This is a prospective study.

Methods: Twenty patients and 20 controls were enrolled into the study.

[Protective effect of total glycosides of Ranunculus japonicus on myocardial ischemic-reperfusion injury in isolated rat hearts].

Objectives: To study the protective effect of total glycosides of Ranunculus japonicus (TGRJ) on myocardial ischemic-reperfusion injury in isolated rat hearts.

Methods: The SD rats were randomly divided into normal control group, ischemia-reperfusion group, and TGRJ in 0.05, 0.

Inhibition of retinopathy of prematurity in rat by intravitreal injection of sorafenib.

Aim: To investigate the effect of intravitreal injection administered sorafenib, a multikinase inhibitor, in a rat model of oxygen-induced retinopathy (OIR).

Methods: Seven-day-old Sprague-Dawley rats (n=144) were randomly assigned to six groups. Group A received normal partial oxygen pressure and groups B, C, D, E and F were exposed to hyperoxia (75±2)% from postnatal 7d (P7) to P12 to induce retinopathy of prematurity.

Clinical observation on removal of small foreign bodies touching the optic nerve in the deep orbital region: a case series.

Purpose: Five patients presented to our institution with deep intraorbital foreign bodies adjacent to the optic nerve. We removed all foreign bodies by lateral orbitotomy to evaluate the treatment and the benefits of their extraction.

Methods: The clinical outcomes of the five patients were retrospectively analyzed.

Posterior chamber phakic intraocular lens implantation for high myopia.

Aim: To evaluate the efficacy, safety and stability of posterior chamber phakic intraocular lens implantation for the correction of high myopia.

Methods: Retrospective case review of 82 eyes (43 patients) undergoing implantable Collamer lens (ICL) placement by a single surgeon (Xiao-Wei Gao) to correct preoperative mean spherical equivalents between -9.00 diopter (D) and -23.

Comparative study of modified and conventional secondary hydroxyapatite orbital implantations.

Aim: To compare the clinical effects of the modified and conventional secondary hydroxyapatite orbital implantations.

Methods: A total of 40 patients who had received eye enucleation were equally randomized into the modified and conventional groups. Twenty patients were treated by conventional method.

Effects of aminoguanidine on retinal apoptosis in mice with oxygen-induced retinopathy.

Aim: To explore the protective effects of aminoguanidine (AG) on retinal apoptosis in mice with oxygen-induced retinopathy (OIR).

Methods: A total of 80 C57BL/6J mice, aged 7 days, were randomly divided into four groups: normal, high oxygen, high oxygen saline and high oxygen treated with AG. In the normal group, mice were housed in normoxic conditions from postnatal day P7 to P17.

Mechanism of immune tolerance induced by donor derived immature dendritic cells in rat high-risk corneal transplantation.

Aim: To study the role of immature dendritic cells (imDCs) on immune tolerance in rat penetrating keratoplasty (PKP) in high-risk eyes and to investigate the mechanism of immune hyporesponsiveness induced by donor-derived imDCs.

Methods: Seventy-five SD rats (recipient) and 39 Wistar rats (donor) were randomly divided into 3 groups: control, imDC and mature dendritic cell (mDC) group respectively. Using a model of orthotopic corneal transplantation in which allografts were placed in neovascularized high-risk eyes of recipient rat.

[Clinical analysis for the treatment of 63 cases of orbital tumors in Xinjiang].

Objective: To analyse the clinical features, histopathological classifications, treatments and prognosis of orbital tumors in Xinjiang.

Methods: The authors retrospectively analyse 63 patients (64 eyes) with orbital tumors, including all nationalities which had been received and treated in ophthalmic department of the PLA 474th Hospital during 2005 to 2009.

Results: The nationality composition in our cases was 37 Han, 16 Uygur (16 eyes), 4 Hui, 3 Mongol, and 3 Kazak.

Experimental study on the treatment of rabbit corneal melting after alkali burn with Collagen cross-linking.

Aim: To evaluate the effect of Collagen cross-linking on the prevention of melting in rabbit corneas after alkali burn.

Methods: Twenty New Zealand white rabbits were randomly divided into model control group and collagen cross-linking treatment group. The second group of rabbits received collagen cross linked treatment.

Adhesion of cavernous hemangioma in the orbit revealed by CT and MRI: analysis of 97 cases.

Aim: To assess features of cavernous hemangioma (CH) in the orbit revealed by CT and MRI and summarize prediction of preoperative CT and MRI for the adhesion degree of CH in the orbit.

Methods: A total of 97 patients with pathologically confirmed CH in the orbit were examined with axial and coronal CT scan, and axial, coronal, sagittal, and enhanced fat suppression MRI scan. CT and MRI findings and intraoperative adhesion degrees were retrospectively analyzed.

Effects of minocycline on expression of bcl-2, bax in early retinal neuropathy of diabetes in rats.

Aim: To study the effect of the minocycline (MNC) on expression of bcl-2, bax in retinal nerve cells of rat with diabetes.

Methods: Male SD rats were randomly divided into negative control group, model control group and MNC treated group. Diabetes model was established by intraperitoneal injection of 60 mg/kg streptozotocin (STZ).

[Status analysis of nutrients and eutrophication assessment in Shenzhen coastal waters].

Based on the field data of Shenzhen coastal water quality in 2002-2007, variation characteristics of nutrients including NH4+ -N, NO3- -N, NO2- -N, PO4(3-) -P and DIN were presented. And the correlationships between nutrients and pH, salinity were also investigated. Furthermore, eutrophication index (E), organic pollution index (A) and potential eutrophication were employed to assess the eutrophication degree of Shenzhen coastal waters.