Nrf2 Deficiency Exacerbates Cognitive Impairment and Reactive Microgliosis in a Lipopolysaccharide-Induced Neuroinflammatory Mouse Model.

The transcription factor Nrf2 is a central regulator of anti-inflammatory and antioxidant mechanisms that contribute to the development and progression of various neurological disorders. Although the direct and indirect Nrf2 regulatory roles on inflammation have been reviewed in recent years, the in vivo evidence of Nrf2 function on lipopolysaccharide (LPS)-induced cognitive decline and characteristic alterations of reactive microglia and astrocytes remains incomplete. During the 3-5 days after LPS or saline injection, 5-6-month-old wildtype (WT) and Nrf2 C57BL/6 mice were subjected to the novel object recognition task.

[Effect of propofol and operative trauma on neurodevelopment and cognitive function of developing brain in rats].

Objective: To investigate the effect of propofol and operative trauma on the neurodevelopment and cognitive function of the developing brain and its mechanism.

Methods: A total of 104 postnatal day 13 Sprague-Dawley rats were randomly divided into 4 groups: control group (treated by 7.5 mL/kg saline and sham surgery), propofol group (treated by 75 mg/kg propofol), surgery group (with abdominal surgery under local anesthesia) and propofol+surgery group (with abdominal surgery under local anesthesia plus 75 mg/kg propofol anesthesia).

Rapid decolorization of dye Orange G by microwave enhanced Fenton-like reaction with delafossite-type CuFeO.

Bimetallic oxide CuFeO as a new heterogeneous catalyst has shown much higher catalytic ability for activating peroxide than single-metal oxides. The present work demonstrated a synergistic microwave (MW) enhanced Fenton-like process with CuFeO for rapid decolorization of azo dye Orange G (OG). The MW irradiation dramatically enhanced the OG degradation efficiency, achieving 99.

[Comparative study on accumulation of Ah-receptor agonists in contaminated soil based on EROD bioassay and chemical analysis].

The potential ecological risk by wastewater or reclaimed water for irrigation is of great concerns in recent years, but little work was done on the chronic toxicities through long term accumulation of persistent organic chemicals in soil. In present work, concentration of Ah-receptor agonists in soil organic extract was measured by an ethoxyresorfin O-deethylase (EROD) bioassay, which was calibrated and expressed by the 2, 3, 7, 8-tetrachlorodibenzo- p-dioxin (2, 3, 7, 8-TCDD) toxic equivalent (TEQbio). Simultaneously, 16 PAHs in soil were analyzed and their TEQs (total as TEQ(PAHs)) were calculated according to their toxic equivalent factors (TEFs) cited from literature.

Genotoxic risk identification of soil contamination at a major industrialized city in northeast China by a combination of in vitro and in vivo bioassays.

The present study evaluated the genotoxicity of field soils in the Tianjin area, one of the most industrialized contaminated areas in northeast China. The genotoxicity of organic extracts of 41 soils was assayed by an in vitro SOS/ umu bioassay with Salmonella typhimurium TA 1535/pSK 1002. From the 41 soil samples, 11 samples were selected to confirm the genotoxic effect by in vivo single-cell gel electrophoresis (comet assay) using earthworms (Eisenia fetida).

[Genotoxicity of soil irrigated by wastewater using umu/SOS test].

umu/SOS test is used to screen the accumulation of genotoxic substances in soil irrigated with wastewater in urban Beijing. The relative genotoxicity is evaluated based on the weight of soil used for extraction that could result in a positive reaction (or the induction ratio R equals 2) and the characteristics of genotoxic substances could be evaluated by the slop of linear part of the dose/effect relationship, R reaches the maximum when the amount of soil in each cell is about 10 mg. In a case study on a wastewater or reclaimed water irrigation area, the genotoxicity of soil samples could be observed and it may be attributed to the accumulation of PAHs, according to relevant information.