Dysregulation of mRNA profile in cisplatin-resistant gastric cancer cell line SGC7901.

Aim: To explore novel therapeutic target of cisplatin resistance in human gastric cancer.

Methods: The sensitivity of SGC7901 cells and cisplatin-resistant SGC7901 cells (SGC7901/DDP) for cisplatin were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. High-quality total RNA which isolated from SGC7901/DDP cells and SGC7901 cells were used for mRNA microarray analysis.