Spatiotemporal distribution, environmental correlation and health risk analysis of (Diptera: Culicidae) in Beijing, China.

The Giles, 1901 (Diptera: Culicidae) is major vector of Japanese encephalitis (JE) in China, and this study aimed to uncover the vector's spatiotemporal distribution and environmental correlation in Beijing. In study area, the Remote Sensing (RS), Global Position System (GPS), and Geographic Information System (GPS) were used to clarify the distribution characteristics of vector on spatial and temporal scales, and regressions analysis of cross-sectional study was performed to detect the environmental factors linked with the density and presence of . In study area, the scenic area was the major environmental area for breeding of the vector, August was the primary peak month, the new urban development area (NUDA) was major distribution subarea of Beijing, and the vector could be detected throughout the subarea of Beijing from June to September.

Symptomatic and Asymptomatic SARS-CoV-2 Infection and Follow-up of Neutralizing Antibody Levels.

Objective: To investigate neutralizing antibody levels in symptomatic and asymptomatic patients with coronavirus disease 2019 (COVID-19) at 6 and 10 months after disease onset.

Methods: Blood samples were collected at three different time points from 27 asymptomatic individuals and 69 symptomatic patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Virus-neutralizing antibody titers against SARS-CoV-2 in both groups were measured and statistically analyzed.

Recombinant tegumental protein Shistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against Schistosoma japonicum.

A polyhistidine-tagged recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein (SVLBP) from adult Schistosoma japonicum was expressed in Escherichia coli. The affinity purified rSVLBP was used to vaccinate mice. The worm numbers and egg deposition recovered from the livers and veins of the immunized mice were 33.

[Protective immunity of the recombinant Schistosoma japonicum specific very low density lipoprotein binding protein as a vaccine candidate].

Objective: To investigate the protective immunity against Schistosoma japonicum in mice immunized with recombinant specific very low density lipoprotein binding protein (SVLBP) and its potential as vaccine candidate.

Methods: Recombinant SVLBP antigen was over-expressed under IPTG induction and purified by Ni-NTA affinity chromatography. C57BL/6 mice were immunized three times with purified reSVLBP complexed with Freund's adjuvant, at biweekly intervals.